Excavation of R2R3-MYB Transcription Factor and Its Response to Drought Stress in Bothriochloa ischaemum

doi:10.11733/j.issn.1007-0435.2020.06.035

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (6): 1784-1790.DOI: 10.11733/j.issn.1007-0435.2020.06.035

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Excavation of R2R3-MYB Transcription Factor and Its Response to Drought Stress in Bothriochloa ischaemum

LI Chun-yan¹, WANG Xi¹, ZHOU Sheng-hua¹, LI Xi¹, ZHONG Hua², DONG Kuan-hu²

1. Shanxi Agricultural University(Shanxi Academy of Agricultural Sciences), Taiyuan, Shanxi Province 030032, China;
2. College of Agro-Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2020-04-23 Revised:2020-07-24 Published:2020-12-02

白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究

李春艳¹, 王曦¹, 周胜花¹, 李希¹, 钟华², 董宽虎²

1. 山西农业大学山西省农业科学院, 山西太原 030032;
2. 山西农业大学草业学院, 山西太谷 030801

通讯作者: 董宽虎
作者简介:李春艳(1978-),女,山西岚县人,博士,副研究员,主要从事牧草遗传育种研究,E-mail:licyjzgjkx@163.com
基金资助:
山西省农业科学院博士基金项目（YBSJJ2008）资助

Abstract

Abstract: This paper analyzed the expression pattern of the R2R3-MYB transcription factors of B. ischaemum under drought stress,through searching,comparison,functional domain polymorphism analysis,phylogenetic tree construction and gene expression analysis. A total of 43 R2R3-MYB transcription factors with highly conserved[W]-X(19)-[W]-X(19)-[W]-X(n) -[W]-X(18)-[W] were isolated from B. ischaemum. They were mainly involved in growth and development,secondary metabolism and stress responses. Through further analysis,there were eight R2R3-MYB transcription factors that may participate in drought stress response. This research lays a foundation for the functional analysis and application of R2R3-MYB.

Key words: Bothriochloa ischaemum, Transcriptomic database, Drought stress, R2R3-MYB

摘要： 本文以白羊草（Bothriochloa ischaemum）为材料，利用其目前已有的转录组序列，通过搜索、比对、功能域多态性分析、进化树构建和基因表达分析等手段，对白羊草R2R3-MYB型转录因子进行挖掘和干旱胁迫下的表达模式分析。结果从白羊草挖掘出43个R2R3-MYB转录因子，它们具有高度保守的[W]-X（19）-[W]-X（19）-[W]-X（n）-[W]-X（18）-[W]结构，主要参与生长发育、次生代谢和逆境响应等生物过程。通过进一步的分析，本研究筛选出8个可能参与干旱胁迫响应的R2R3-MYB转录因子。这为白羊草R2R3-MYB基因的功能研究和开发利用奠定了基础。

关键词: 白羊草, 转录组数据库, 干旱胁迫, R2R3-MYB转录因子

CLC Number:

Q945

LI Chun-yan, WANG Xi, ZHOU Sheng-hua, LI Xi, ZHONG Hua, DONG Kuan-hu. Excavation of R2R3-MYB Transcription Factor and Its Response to Drought Stress in Bothriochloa ischaemum[J]. Acta Agrestia Sinica, 2020, 28(6): 1784-1790.

李春艳, 王曦, 周胜花, 李希, 钟华, 董宽虎. 白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究[J]. 草地学报, 2020, 28(6): 1784-1790.

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Excavation of R2R3-MYB Transcription Factor and Its Response to Drought Stress in Bothriochloa ischaemum

白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究

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