Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (6): 1784-1790.DOI: 10.11733/j.issn.1007-0435.2020.06.035

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Excavation of R2R3-MYB Transcription Factor and Its Response to Drought Stress in Bothriochloa ischaemum

LI Chun-yan1, WANG Xi1, ZHOU Sheng-hua1, LI Xi1, ZHONG Hua2, DONG Kuan-hu2   

  1. 1. Shanxi Agricultural University(Shanxi Academy of Agricultural Sciences), Taiyuan, Shanxi Province 030032, China;
    2. College of Agro-Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China
  • Received:2020-04-23 Revised:2020-07-24 Published:2020-12-02

白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究

李春艳1, 王曦1, 周胜花1, 李希1, 钟华2, 董宽虎2   

  1. 1. 山西农业大学山西省农业科学院, 山西 太原 030032;
    2. 山西农业大学草业学院, 山西 太谷 030801
  • 通讯作者: 董宽虎
  • 作者简介:李春艳(1978-),女,山西岚县人,博士,副研究员,主要从事牧草遗传育种研究,E-mail:licyjzgjkx@163.com
  • 基金资助:
    山西省农业科学院博士基金项目(YBSJJ2008)资助

Abstract: This paper analyzed the expression pattern of the R2R3-MYB transcription factors of B. ischaemum under drought stress,through searching,comparison,functional domain polymorphism analysis,phylogenetic tree construction and gene expression analysis. A total of 43 R2R3-MYB transcription factors with highly conserved[W]-X(19)-[W]-X(19)-[W]-X(n) -[W]-X(18)-[W] were isolated from B. ischaemum. They were mainly involved in growth and development,secondary metabolism and stress responses. Through further analysis,there were eight R2R3-MYB transcription factors that may participate in drought stress response. This research lays a foundation for the functional analysis and application of R2R3-MYB.

Key words: Bothriochloa ischaemum, Transcriptomic database, Drought stress, R2R3-MYB

摘要: 本文以白羊草(Bothriochloa ischaemum)为材料,利用其目前已有的转录组序列,通过搜索、比对、功能域多态性分析、进化树构建和基因表达分析等手段,对白羊草R2R3-MYB型转录因子进行挖掘和干旱胁迫下的表达模式分析。结果从白羊草挖掘出43个R2R3-MYB转录因子,它们具有高度保守的[W]-X(19)-[W]-X(19)-[W]-X(n)-[W]-X(18)-[W]结构,主要参与生长发育、次生代谢和逆境响应等生物过程。通过进一步的分析,本研究筛选出8个可能参与干旱胁迫响应的R2R3-MYB转录因子。这为白羊草R2R3-MYB基因的功能研究和开发利用奠定了基础。

关键词: 白羊草, 转录组数据库, 干旱胁迫, R2R3-MYB转录因子

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