Abstract: Taking the epicotyl,cotyledon,and hypocotyl of sainfoin(Onobrychis viciifolia Scop.) seedlings as explants,the effects of different plant hormones on tissue culture and plant regeneration system of sainfoin were studied using orthogonal design method. The results showed that,the influence degree of hormone on callus induction was NAA > ZT > 6-BA,and the best medium for inducing explants to produce callus was MS+1.0 mg·L^-1 6-BA+0.5 mg·L^-1 NAA+ 0.2 mg·L^-1 ZT with the induction rate of 94.5%; Results of bud differentiation showed that the influence sequence of three factors was NAA>6-BA>ZT,MS+0.5 mg·L^-1 6-BA+2.5 mg·L^-1 ZT was the optimum induction medium and adventitious shoots induction rate could reach 88.9%; The optimal medium combination to induce adventitious shoot rooting was 1/2 MS + 0.5 mg·L^-1 NAA + 0.2 mg·L^-1 IBA-K,and the induction rate was up to 45%.

Key words: Sainfoin, Orthogonal experimental design, Callus tissue, Plant regeneration

摘要： 以红豆草（Onobrychis viciifolia Scop.）无菌苗的上胚轴、真叶和下胚轴为外植体，根据正交试验设计方法，研究不同植物激素对红豆草组织培养及植株再生体系的影响。结果表明：激素对愈伤诱导的影响程度为萘乙酸（Naphthylacetic acid，NAA）>玉米素（Zeatin，ZT）> 6-苄氨基嘌呤（6-Benzylaminopurine，6-BA），诱导外植体产生愈伤组织的最佳培养基为MS+1.0 mg·L^-1 6-BA+0.5 mg·L^-1 NAA+0.2 mg·L^-1 ZT，诱导率达94.5%；对不定芽分化率的影响程度为NAA > 6-BA > ZT，诱导愈伤组织分化不定芽的最佳培养基为MS+0.5 mg·L^-1 6-BA+2.5 mg·L^-1 ZT，诱导率达88.9%；诱导不定芽生根的最佳培养基为1/2 MS+0.5 mg·L^-1 NAA+0.2 mg·L^-1吲哚丁酸钾（Indole-3-butyric acid potassium，IBA-K），诱导率高达45%。

关键词: 红豆草, 正交试验法, 愈伤组织, 植株再生