Abstract: In order to identify the pathogen causing root rot on Lathyrus sativus,the infected plants were collected during August of 2018 in Nanchong city,Sichuan province. The pathogen was isolated from tissue， single spore was purified,and pathogenicity was tested in the laboratory. Pathogenic isolates were identified by morphological characteristics and EF-1α gene sequence analysis. The results showed that 39 isolates were obtained from infected plant,of which three isolates including Shan-27,Shan-28 and Shan-29 had strong pathogenicity to L.sativus,accounting for 7.69%. The above three strains were all close to Fusarium graminearum species complex in morphology. Their translation elongation factor (EF-1α) were amplified by PCR,sequenced,and compared with Blast in GenBank. The results showed that their EF-1α were clustered in the same group with F. asiaticum, and their homology were more than 99%. Therefore,according to the morphological characteristics of the isolates and the sequence analysis of EF-1α gene,the three pathogenic isolates causing L.sativus root rot were identified as F. asiaticum.

Key words: Lathyrus sativus, Root rot, Fusarium asiaticum, Identification of pathogen, Translation elongation factor

摘要： 为了明确山黧豆（Lathyrus sativus）根腐病病原菌的分类地位，本研究于2018年8月在四川省南充市采集山黧豆根腐病病样，经组织分离法进行分离纯化，采用形态学和翻译延伸因子（Translation elongation factor 1-agene，EF-1α）基因序列分析相结合的方法对致病菌进行鉴定。结果表明：从采集的山黧豆根腐病病样上共分离获得39株分离物，其中3株分离物Shan-27，Shan-28和Shan-29对山黧豆具有强致病性，分离频率为7.69%；这3株致病菌在形态学上均接近于禾谷镰孢菌复合种（Fusarium graminearum species complex），而对其翻译延伸因子（EF-1α）序列分析发现，其均与亚洲镰孢菌聚在同一分支上，同源性达99%以上。因此，根据菌株形态学特征和EF-1α基因序列分析结果，将引致山黧豆根腐病的病原菌确定为亚洲镰孢菌（Fusarium asiaticum）。

关键词: 山黧豆, 根腐病, 亚洲镰孢菌, 病原鉴定, EF-1α