Research Progresses on Detection and Quantification of Plant Pathogenic Fungi Fusarium oxysporum

doi:10.11733/j.issn.1007-0435.2021.07.030

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (7): 1599-1604.DOI: 10.11733/j.issn.1007-0435.2021.07.030

Research Progresses on Detection and Quantification of Plant Pathogenic Fungi Fusarium oxysporum

DONG Chao, FANG Xiang-ling

State Key Laboratory of Glassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730020, China

Received:2020-12-27 Revised:2021-02-19 Online:2021-07-15 Published:2021-07-31

植物病原真菌尖孢镰刀菌检测与定量研究进展

董超, 方香玲

草地农业生态系统国家重点实验室, 农业农村部草牧业创新重点实验室, 兰州大学草地农业科技学院, 甘肃兰州 730020

通讯作者: 方香玲,E-mail:xlf@lzu.edu.cn
作者简介:董超(1998-),男,汉族,内蒙古兴安盟人,硕士研究生,主要从事草地保护学研究,E-mail:dongch20@lzu.edu.cn
基金资助:
国家自然科学基金（31802126）；国家绿肥产业技术体系（CARS-22）；青海省科技厅基础研究计划创新团队项目（2021-ZJ-901）资助

Abstract

Abstract: Fusarium oxysporum is one serious soil-borne pathogenic fungus and is listed as one of the top ten plant fungal pathogens in the world. This fungus can infect more than 100 economically important crops such as cotton,soybean,watermelon,banana,tomato and alfalfa,causing wilt and root rot. The detection and quantification of F. oxysporum in soil and plant root tissues is the basis for early diagnosis and effective control of the disease. This article summarizes the research progress and application of F. oxysporum detection and quantification methods at domestic and overseas,mainly including medium colony plate dilution method,conventional PCR and real-time fluorescent quantitative PCR,etc. This review provides theoretical guidance for F. oxysporum detection and quantification in agriculture.

Key words: Fusarium oxysporum, Detection, Quantification, Real-time fluorescent quantitative PCR

摘要： 尖孢镰刀菌（Fusarium oxysporum）是一种危害严重的土传病原真菌，被列为世界十大植物病原真菌之一。该菌能够侵染棉花（Gossypium hirsutum）、大豆（Glycine max）、西瓜（Citrullus lanatus）、香蕉（Musa nana）、番茄（Lycopersicon esculentum）和苜蓿（Medicago sativa）等100多种具有重要经济价值的作物，引起枯萎病和根腐病等。对土壤和植物根组织中的尖孢镰刀菌进行检测和定量是病害早期诊断和有效防治的基础。本文对国内外关于尖孢镰刀菌检测和定量方法（主要包括培养基菌落平板稀释法、常规PCR和实时荧光定量PCR等）及其应用进行总结，旨在对农牧业生产中尖孢镰刀菌检测和定量提供理论指导。

关键词: 尖孢镰刀菌, 检测, 定量, 实时荧光定量PCR

CLC Number:

S432.4+4

DONG Chao, FANG Xiang-ling. Research Progresses on Detection and Quantification of Plant Pathogenic Fungi Fusarium oxysporum[J]. Acta Agrestia Sinica, 2021, 29(7): 1599-1604.

董超, 方香玲. 植物病原真菌尖孢镰刀菌检测与定量研究进展[J]. 草地学报, 2021, 29(7): 1599-1604.

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Research Progresses on Detection and Quantification of Plant Pathogenic Fungi Fusarium oxysporum

植物病原真菌尖孢镰刀菌检测与定量研究进展

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