Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (10): 2214-2220.DOI: 10.11733/j.issn.1007-0435.2021.10.012

Previous Articles     Next Articles

Response of Physiological and Biochemical Characteristics of Alfalfa with Different Root Collar Diameter to Low Temperature Stress

ZHU Ai-min1, HUANG Wei-li2, HAN Guo-dong1, ZHANG Yu-xia3, WANG Xian-guo4, WU Qian1   

  1. 1. Key Laboratory of Grassland Resources of the Ministry of Education, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of the Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Grassland Management and Utilization, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010010, China;
    2. Inner Mongolia Academy of Forestry, Hohhot, Inner Mongolia 010010, China;
    3. College of Agriculture, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028041, China;
    4. College of animal science and technology, China Agricultural University, Beijing 100083, China
  • Received:2021-04-07 Revised:2021-05-16 Online:2021-10-15 Published:2021-11-05

不同根颈直径苜蓿生理生化特性对低温胁迫的响应

朱爱民1, 黄卫丽2, 韩国栋1, 张玉霞3, 王显国4, 武倩1   

  1. 1. 内蒙古农业大学草原与资源环境学院, 草地资源教育部重点实验室, 农业部饲草栽培、加工与高效利用重点实验室, 内蒙古自治区草地管理与利用重点实验室, 内蒙古 呼和浩特 010010;
    2. 内蒙古自治区林业科学研究院, 内蒙古 呼和浩特 010010;
    3. 内蒙古民族大学农学院, 内蒙古 通辽 028041;
    4. 中国农业大学动物科技学院, 北京 100083
  • 通讯作者: 韩国栋,E-mail:nmghanguodong@163.com
  • 作者简介:朱爱民(1992-),男,安徽省宿州市人,博士研究生,主要从事牧草种质资源与利用方向研究,E-mail:m15344081730@163.com
  • 基金资助:
    内蒙古自治区科技储备项目（2018MDCB032）；内蒙古自然科学基金项目（2020MS03081）；中国博士后科学基金（2020M670713）资助

PDF

Abstract: In order to explore the relationship between cold resistance and the physiological and biochemical characteristics of alfalfa (Medicago sativa L.) with different root collar diameters, ‘Gongnong No.1’ alfalfa was taken as experimental material, and the root system of alfalfa with different collar diameter (3.50 mm, 5.25 mm, 7.00 mm) was treated with low temperature stress (4℃, -10℃, -15℃, -20℃). The root collar activity, non-structural carbon and nitrogen content and antioxidant enzyme activity of alfalfa were determined. The results showed that low temperature stress had extremely significant effects on alfalfa root collar activity, unstructured carbon and nitrogen, unstructured carbon nitrogen ratio (C/N) and catalase (CAT) activity (P<0.01);The activity of root collar of alfalfa with different root collar diameters decreased with the decrease of stress temperature, and the content of soluble sugar and soluble protein in root collar increased first and then decreased, and then increased. The content of starch, C/N and CAT activity increased first and then decreased;The activity of alfalfa root collar was significantly correlated with the content of soluble sugar, soluble protein, CAT activity and C/N (P<0.05);This study suggested that alfalfa with larger root collar diameter had stronger cold resistance, and it could adapt to low temperature stress by regulating soluble protein, soluble sugar content, CAT activity and C/N in root collar.

Key words: Root collar diameter, Alfalfa, Cold resistance, Physiological and biochemical characteristics

摘要： 为了探究不同颈粗苜蓿（Medicago sativa L.）的抗寒性与其根颈中生理生化特性的关系。以‘公农1号’紫花苜蓿为试验材料，对不同颈粗（3.50 mm，5.25 mm，7.00 mm）苜蓿根系进行低温胁迫（4℃，-10℃，-15℃，-20℃）处理，测定了苜蓿根颈活力、非结构碳氮含量和抗氧化酶活性。结果表明：低温胁迫对苜蓿根颈活力、非结构碳氮含量、非结构碳氮比（Cnstructured carbon nitrogen ratio，C/N）及过氧化氢酶（Catalase，CAT）活性均有极显著影响（P<0.01）；随低温胁迫的增加不同颈粗的苜蓿根颈活力均降低，可溶性糖和可溶性蛋白含量均呈增加-降低-增加的变化，淀粉含量、C/N和CAT活性均呈先增加后降低的趋势；苜蓿根颈活力大小与根颈中可溶性糖含量、可溶性蛋白含量、CAT活性及C/N显著相关（P<0.05）。研究认为，根颈直径大的苜蓿抗寒性较强，其通过调控根颈中可溶性蛋白、可溶性糖含量和CAT活性及C/N以适应低温胁迫。

关键词: 根颈直径, 苜蓿, 抗寒性, 生理生化特性

CLC Number: