The Effects of Exogenous Gibberellin (GA3) on the Symbiotic System of Alfalfa Rhizobium

doi:10.11733/j.issn.1007-0435.2022.02.016

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (2): 385-393.DOI: 10.11733/j.issn.1007-0435.2022.02.016

The Effects of Exogenous Gibberellin (GA₃) on the Symbiotic System of Alfalfa Rhizobium

HE Yu-juan¹, LI Dong-qin^1,2, DENG Bo¹, YANG Jun³, Duishanaili·kabutula⁴, YANG Chao⁵

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Jiuquan Forest Technology Services Center, Jiuquan, Gansu Province 735000, China;
3. Urumqi Qilvzhiyuan Garden Development Co., Ltd., Urumqi, Xinjiang 830000, China;
4. Forestry and Grassland Bureau of Tekes, County, Tekes, Xinjiang Province 835500, China;
5. Tekes Branch of the State-owned Forestry Administration of Western Tianshan Mountains, Tekes, Xinjiang 835500, China

Received:2021-07-19 Revised:2021-09-01 Published:2022-03-10

外源赤霉素GA₃施加对紫花苜蓿-根瘤菌共生体系的影响

何玉娟¹, 李东琴^1,2, 邓波¹, 杨军³, 对山艾力·卡布吐拉⁴, 杨超⁵

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 甘肃省酒泉市林果服务中心, 甘肃酒泉 735000;
3. 乌鲁木齐绿之园园林开发有限公司, 新疆乌鲁木齐 830000;
4. 特克斯县林业和草原局, 新疆特克斯 8355005;
5. 天山西部国有林管理局特克斯分局, 新疆特克斯 835500

通讯作者: 邓波,E-mail:dengbo@cau.edu.cn
作者简介:何玉娟(1996-),女,汉族,安徽安庆人,博士研究生,主要从事草地生态研究,E-mail:heyujuan945@163.com
基金资助:
新疆维吾尔自治区“自治区区域协同创新专项(科技援疆计划)”项目(2019E0211)资助

Abstract

Abstract: In order to explore the effect of different concentrations of exogenous gibberellin on the nodulation process of alfalfa-rhizobium and its mechanism of action,different concentrations of exogenous gibberellin were applied to the symbiosis of alfalfa-rhizobium to explore the effect on nodule number,dry and fresh weight,rhizome length,nodule primordium,root hair curling,and flavonoid secretion in the process of nodulation. The main results were as follows: 10^-9mol·L^-1 GA₃ increased the expression of nod gene on rhizobia,which led to the increase of the amount of flavonoids in plant roots and the formation of active nodules,and promoted the number of nodule. 10^-5mol·L^-1GA₃ inhibited the root hair curl process,and reduced the number of nodules. Therefore,appropriate addition of exogenous gibberellin during the nodulation process of alfalfa could improve the nodulation effect and promote crop growth.

Key words: Alfalfa, Rihizobium, Gibberellin, Nodule number

摘要： 为探究不同浓度的外源赤霉素添加对苜蓿(Medicago sativa)-根瘤菌共生体系结瘤过程的影响及其作用机制，本试验在室内控制条件下，外源施加不同浓度的赤霉素，观察其对苜蓿根瘤数、干鲜重、根茎长、苜蓿-根瘤菌共生结瘤过程中根瘤原基、根毛卷曲、类黄酮物质分泌的影响。结果显示：10^-9mol·L^-1的赤霉素可以促进nod基因的表达，引起植株根系黄酮类物质的分泌量增加，诱导形成更多且活性更强的根瘤原基，促进根瘤的形成。10^-5mol·L^-1赤霉素抑制根毛卷曲过程，从而减少根瘤数。因此，在紫花苜蓿结瘤过程中可适当添加外源赤霉素以提高结瘤效果，促进作物生长。

关键词: 紫花苜蓿, 根瘤菌, 赤霉素, 结瘤数量

CLC Number:

Q939.11+4

HE Yu-juan, LI Dong-qin, DENG Bo, YANG Jun, Duishanaili·kabutula, YANG Chao. The Effects of Exogenous Gibberellin (GA₃) on the Symbiotic System of Alfalfa Rhizobium[J]. Acta Agrestia Sinica, 2022, 30(2): 385-393.

何玉娟, 李东琴, 邓波, 杨军, 对山艾力·卡布吐拉, 杨超. 外源赤霉素GA₃施加对紫花苜蓿-根瘤菌共生体系的影响[J]. 草地学报, 2022, 30(2): 385-393.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.02.016

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I2/385

References

[1] 刘薇,赵振芳,冯永君. 植物激素在豆科植物根瘤形成和发育过程中的调控作用[J]. 大豆科学,2013,32(2):262-266
[2] 卢春霞,罗小玲,陈霞. 赤霉素的研究进展[J]. 世界科技研究与发展,2012,34(1):45-45
[3] HAYASHI S，GRESSHOFFP M，FERGUSON B J. Mechanistic action of gibberellins in legume nodulation[J]. Journal of Integrative Plant Biology,2014,56(10):971-978
[4] SAM L,SOFIE G,JEROEN D H,et al. Gibberellins are involved in nodulation of Sesbania rostrata[J]. Plant Physiology,2005,139(3):1366-1379
[5] MAEKAWA T，MAEKAWA-YOSHIKAWA M，TAKEDA N，et al. Gibberellin controls the nodulation signaling pathway in Lotus japonicus[J]. Plant Journal,The,2009,58(2):183-194
[6] LIBAULT M，FARMER A，BRECHENMACHER L，et al. Complete transcriptome of the soybean root hair cell,a single-cell model,and its alteration in response to Bradyrhizobium japonicum infection.[J]. Plant Physiology,2010,152(2):541-52
[7] NUMAN M，BASHIR S，KHAN Y，et al. Plant Growth Promoting Bacteria as an Alternative Strategy for Salt Tolerance in Plants:A Review[J]. Microbiological Research,2018:21-32
[8] BRETT J F,JOHN R,JAMES B R. Nodulation Phenotypes of Gibberellin and Brassinosteroid Mutants of Pea[J]. Plant Physiology,2005,138(4):2396-2405
[9] FERGUSON B J,FOO E,REID R.Relationship between gibberellin,ethylene and nodulation in Pisum sativum[J]. New Phytologist,2011,189(3):829-842
[10] RENE G,FEDOROVA E，BISSELING T. Nod factor signaling genes and their function in the early stages of Rhizobium infection[J]. Current Opinion in Plant Biology,2005,8(4):346-352
[11] PETER M，ATTILA K，EVA K. Gene Expression in Nitrogen-Fixing Symbiotic Nodule Cells in Medicago truncatula and Other Nodulating Plants[J]. The Plant Cell,2019(1):1
[12] MARTIN P. Uptake of bacteria into living plant cells,the unifying and distinct feature of the nitrogen-fixing root nodule symbiosis[J]. Current Opinion in Plant Biology,2018,44:164-174
[13] RIELY B K，JEAN M A,PENMETSA R V，et al. Genetic and genomic analysis in model legumes bring Nod-factor signaling to center stage.[J]. Current Opinion in Plant Biology,2004,7(4):408-413
[14] 于跃,刘静,邓波,等.外源根系分泌物对紫花苜蓿根瘤菌共生体系的影响[J]. 草地学报,2020,28(1):88-94
[15] 靖元孝. 根瘤菌结瘤因子的研究进展[J]. 生命的化学,1997,17(1):17-19
[16] 李莎莎,张志强,王亚芳,等.根瘤菌共生对低温胁迫下紫花苜蓿抗寒生理变化的影响[J]. 草地学报,2016,24(2):377-383
[17] 冯思琼. 茉莉酸甲酯对大豆根部茉莉酸和类黄酮合成的影响[D].广州：华南师范大学,2010:21-23
[18] 周圣骄,宋志远. 植物生长调节剂对大豆根瘤菌生长及功能的调控效应[J]. 生物技术世界,2016(5):83
[19] ALBRECHT C，GEURTS R，LAPEYRIE F，et al. Endomycorrhizae and rhizobial Nod factors both require SYM8 to induce the expression of the early nodulin genes PsENOD5 and PsENOD12A.[J]. Plant Journal,2010,15(5):605-614
[20] CARMEN,BIANCO,ROBERTO,et al. Auxins up regulate nif and fix genes[J]. Plant Signaling & Behavior,2014,5(10):4626-4632
[21] LIU T，LI X，ZHOU Z. Improvement of hydrogen yield by hupR gene knock-out and nifA gene overexpression in Rhodobacter sphaeroides 6016[J]. International Journal of Hydrogen Energy,2010,35(18):9603-9610
[22] MESA S，HAUSER F，FRIBERG M，et al. Comprehensive Assessment of the Regulons Controlled by the FixLJ-FixK2-FixK1 Cascade in Bradyrhizobium japonicum[J]. Journal of Bacteriology,2008,190(20):6568-6579(责任编辑彭露茜)第30卷第2期 Vol.30 No. 2草地学报 ACTAAGRESTIASINICA 2022年 2月

The Effects of Exogenous Gibberellin (GA₃) on the Symbiotic System of Alfalfa Rhizobium

外源赤霉素GA₃施加对紫花苜蓿-根瘤菌共生体系的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Xi-jiang, YANG Chang-fu, JIANG Xue-qian, ZHANG Fan, HE Fei, YANG Qing-chuan, KANG Jun-mei. QTL Analysis of Ash and Mineral Elements in Alfalfa [J]. Acta Agrestia Sinica, 2022, 30(2): 312-319.
[2]	ZHAO Yang-an, LU Guang-xin, DENG Ye, WANG Ying-cheng, MA Kun, YAN Hui-lin, ZHANG Hai-juan, ZHOU Xue-li, DOU Sheng-yun. Effect of Rhizobium Seed Dressing on Growth of Two Alfalfa and Rhizosphere Microbial Diversity [J]. Acta Agrestia Sinica, 2022, 30(2): 370-378.
[3]	HAN Yan-long, LIU Xiao-jing, WANG Jing, WANG Xue, LI Jia-qi. Effects of Nitrogen Application on Root Characteristics and Productivity of Different Alfalfa Cultivars [J]. Acta Agrestia Sinica, 2022, 30(2): 379-384.
[4]	SHEN Xiao-hui. Analysis of DNA Methylation Map of Alfalfa Whole Genome under Two Kinds of Mutagenesis Treatments [J]. Acta Agrestia Sinica, 2022, 30(1): 46-54.
[5]	DU Hong-qi, LOU Zhi-guo, ZOU Jing, FANG Wei-ping, FENG Chang-song. Screening and Functional Identification of Proteins Specifically Expressed in Terminal Buds of Different Fall Dormancy Alfalfa [J]. Acta Agrestia Sinica, 2022, 30(1): 55-61.
[6]	WU Jie-rui, LIU Bing-yi, TE Muerbuhe, SHUANG Shuang, DAI Rui, ZHANG Zhi-qiang. Effects of Thrips Infesting on Amino Acid Composition and Content of Alfalfa [J]. Acta Agrestia Sinica, 2022, 30(1): 62-68.
[7]	WANG Xue, LIU Xiao-jing, ZHAO Ya-jiao, WANG Jing. Dynamic Characteristics of Nitrogen Nutrition in Alfalfa/Sweet Sorghum Intercropping Under Different Root Interaction Intensities [J]. Acta Agrestia Sinica, 2022, 30(1): 76-83.
[8]	ZHAO Wen-jing, ZHANG Hao-yang, XU Yan-ni, LUO Jiao, LIU Mei-jun. Effects of the Alternative Oxidase and Cytochrome Oxidase Pathways on the Germination of Seeds and Growth of Roots of Alfalfa Seedlings under Different Temperatures [J]. Acta Agrestia Sinica, 2022, 30(1): 84-92.
[9]	ZHANG Shi-min, ZHU Hui-sen, ZHAO Jiao-yang, WEI Kai, XIA Fang-shan. Effects of Different Selenium Sources and Concentrations on Seed Germination and Material Transformation of Medicago sativa 'Pianguan' [J]. Acta Agrestia Sinica, 2022, 30(1): 100-107.
[10]	WANG Jing, LIU Xiao-jing, Cheng Tian-tian, Tong Chang-chun, WANG Xue. Study on Leaf Characteristics and Yield Effect of Alfalfa with Different Nitrogen Efficiency [J]. Acta Agrestia Sinica, 2021, 29(9): 1941-1949.
[11]	MENG Xuan-chen, MA Peng-cheng, MA Jie, DUAN Zhen, HAN Yang-yang, ZHANG Ji-yu. Study on the Effect of Mulching Furrow and Fertilization of Alfalfa in Semi-arid Areas of Loess Plateau [J]. Acta Agrestia Sinica, 2021, 29(9): 2098-2106.
[12]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[13]	LI Chun-pin, QIU Yi-hui, XIA Hou-yin, TAN Lu-na, XU Huan-yu, ZHANG Han-wen, GAO Jing-hui. Membership Function Analysis of Low Transpiration and Yield Traits in Alfalfa Multi-paternal Hybrid F₁Generation [J]. Acta Agrestia Sinica, 2021, 29(7): 1416-1422.
[14]	LI Tian-qi, LIN Zhi-ling, LU Xuan, HUANG Jia-yuan, YANG Ning, WO Ye, GAO Kai, LIU Xiao-ni, WANG Xian-guo. Effects of Irrigation Amount on Soil Available Nutrient Distribution and Leaching Loss of Alfalfa in Horqin Sandy Land [J]. Acta Agrestia Sinica, 2021, 29(7): 1454-1461.
[15]	LI Hong, LI Bo, FANG Zhi-jian, YANG Zhao, ZHANG Chao, FENG Cheng-long, JIN Shan-shan, WANG Si-qi, SHI Wan-ying. Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress [J]. Acta Agrestia Sinica, 2021, 29(7): 1469-1476.