Analyzing Cold Acclimation Process of Alfalfa Based on KEGG Pathway

doi:10.11733/j.issn.1007-0435.2022.07.013

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (7): 1721-1730.DOI: 10.11733/j.issn.1007-0435.2022.07.013

Analyzing Cold Acclimation Process of Alfalfa Based on KEGG Pathway

XU Hong-yu^1,2, LI Yu-ying¹

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Key Laboratory of Model Innovation in Effcient Forage Production, Ministry of Agriculture and Rural Affairs, Taigu, Shanxi Province 030801, China

Received:2022-04-11 Revised:2022-05-31 Published:2022-08-02

基于KEGG通路分析紫花苜蓿的冷适应过程

徐洪雨^1,2, 李钰莹¹

1. 山西农业大学草业学院, 山西太谷 030801;
2. 农业农村部饲草高效生产模式创新重点实验室, 山西太谷 030801

通讯作者: 李钰莹,E-mail:liyuy_ing@163.com
作者简介:徐洪雨(1986-)，男，汉族，内蒙古赤峰人，讲师，博士，主要从事牧草逆境生理响应方面的研究，E-mail:xuhongyu@sxau.edu.cn
基金资助:
山西省优秀博士来晋工作奖励资金科研项目(SXBYKY2021017)；山西农业大学科技创新基金项目(2020BQ64)；国家自然科学基金项目(32101450)资助

Abstract

Abstract: Low temperature is a limiting factor affecting the planting and promotion of alfalfa in China,and the adaptation mechanism to low temperature is still not fully understood. In this study,the gene expression of 'ZhaoDong' and 'WL440HQ' alfalfa before and after cold acclimation was determined by high-throughput sequencing technology. After screening differentially expressed genes,the biological pathways related to cold adaptation and freezing tolerance of alfalfa were analyzed to explore the possible reasons for the difference in freezing tolerance of alfalfa after cold acclimation. The results showed that cellular signal transduction and substance metabolism (including sugar,protein,lipid and so on) were not only the response of alfalfa to cold acclimation,but also related to the improvement of freezing tolerance,such as plant hormone signal transduction,starch and sucrose metabolism and MAPK signaling pathway-plant. The difference in freezing tolerance of alfalfa after cold acclimation was the result of the joint action of various biological pathways,which mainly involved substance metabolism (including fatty acids,amino acids and so on) and energy metabolism,especially the metabolism of amino acid and fatty acid. This study would provide data support for further research on cold resistance mechanism of alfalfa.

Key words: Alfalfa, Cold hardiness, Transcriptome, Cold acclimation

摘要： 低温是影响我国苜蓿(Medicago stavia L.)种植推广的限制性因素，且苜蓿对低温的冷适应机制亦不完全清楚。本研究利用高通量测序技术，测定‘肇东’和‘WL440HQ’苜蓿在冷适应前后的基因表达。筛选差异表达基因后，分析与冷适应低温环境和苜蓿抗寒性提高有关的生物通路以及造成冷适应后苜蓿抗寒性出现差异的可能原因。结果表明：细胞信号转导和物质代谢(包括糖、蛋白质和脂等)等通路，不仅是苜蓿对冷适应低温环境的响应，还可能与其抗寒性的提高有关，如植物激素信号转导、淀粉和蔗糖代谢和植物MAPK信号通路等。冷适应后苜蓿抗寒性出现差异是多种生物通路共同作用下的结果，其中主要涉及物质代谢(包括脂肪酸和氨基酸等)和能量代谢等通路，特别是氨基酸和脂肪酸代谢。本研究将为苜蓿抗寒机制的深入研究提供数据支持。

关键词: 紫花苜蓿, 抗寒性, 转录组, 冷适应

CLC Number:

S963.22+3.3

XU Hong-yu, LI Yu-ying. Analyzing Cold Acclimation Process of Alfalfa Based on KEGG Pathway[J]. Acta Agrestia Sinica, 2022, 30(7): 1721-1730.

徐洪雨, 李钰莹. 基于KEGG通路分析紫花苜蓿的冷适应过程[J]. 草地学报, 2022, 30(7): 1721-1730.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.07.013

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I7/1721

References

[1] YANG S S,TU Z J,CHEUNG F,et al.Using RNA-Seq for gene identification,poly morphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stems[J].BMC Genomics,2011(12):199-218
[2] DONG X,IM S B,LIM Y P,et al.Comparative transcriptome profiling of freezing stress responsiveness in two contrasting Chinese cabbage genotypes,Chiifu and Kenshin[J].Genes Genomics,2014(36):215-227
[3] CASTONGUAY Y,CLOUTIER J,BERTRAND A,et al.SRAP polymorphisms associated with superior freezing tolerance in alfalfa (Medicago sativa spp.sativa)[J].Theoretical and Applied Genetics,2010(120):1611-1619
[4] 王旭东,韩润英,翟天慧,等.通辽市紫花苜蓿越冬存在的问题以及提高返青率的措施[J].饲料广角,2013(2):47-48
[5] 孙洪仁,武瑞鑫,李品红,等.中国草都紫花苜蓿越冬返青成败的关键原因[J].中国奶牛,2015(18):15-18
[6] 朱爱民,张玉霞,王显国,等.沙地生境不同苜蓿品种形态特征对低温的响应及其与抗寒性关系[J].草地学报,2018,26(6):1400-1408
[7] LIAO P,CHEN Q F,CHYE M L.Transgenic Arabidopsis Flowers Overexpressing Acyl-CoA-Binding Protein ACBP6 are Freezing Tolerant[J].Plant and Cell Physiology,2014,55(6):1055-1071
[8] SALTVEIT M E.Discoveries in Plant Biology:Discovery of chilling injury[M].Singapore:World Scientific,2000:423-448
[9] VEENA S,INGE F,JAS S,et al.Cold-activation of Brassica napus BN115 promoter is mediated by structural changes in membranes and cytoskeleton,and requires Ca²⁺ influx[J].The Plant Journal,2001,27(1):1-12
[10] KORN M,GÄRTNER T,ERBAN A,et al.Predicting Arabidopsis freezing tolerance and heterosis in freezing tolerance from metabolite composition[J].Molecular Plant,2010,3(1):224-235
[11] WINFIELD M O,LU C G,WILSON I D,et al.Plant responses to cold:transcriptome analysis of wheat[J].Plant Biotechnology Jounal,2010,8(7):749-771
[12] ANTIKAINEN M,GRIFFITH M.Antifreeze protein accumulation in freezing-tolerant cereals[J].Physiologia Plantarum,1997,99(3):423-432
[13] YOSHIMUNE K,GALKIN A,KULAKOVA L,et al.Cold-active DnaK of an Antarctic psychrotroph Shewanella sp.Ac10 supporting the growth of dnaK-null mutant of Escherichia coli at cold temperatures[J].Extremophiles,2005(9):145-150
[14] THOMASHOW M F.So what's new in the field of plant cold acclimation?Lots![J].Plant Physiology,2001,125(1):89-93
[15] TAYEH N,BAHRMAN N,SELLIER H,et al.A tandem array of CBF/DREB1 genes is located in a major freezing tolerance QTL region on Medicago truncatula chromosome 6[J].BMC Genomics,2013(14):814-830
[16] MARUYAMA K,TAKEDA M,KIDOKORO S,et al.Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated by DREB1A and DREB2A[J].Plant Physiology,2009(150):1972-1980
[17] NOVILLO F,ALONSO J M,ECKER J R.CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(11):3985-3990
[18] WU J,ZHANG Y,YIN L,et al.Linkage of cold acclimation and disease resistance through plant-pathogen interaction pathway in Vitis amurensis grapevine[J].Functional&Intergrative Genomics,2014(14):741-755
[19] BARAH P,JAYAVELU N D,RASMUSSEN S,et al.Genome-scale cold stress response regulatory networks in ten Arabidopsis thaliana ecotypes[J].BMC Genomics,2013(14):722
[20] SVENSSON J T,CROSATTI C,CAMPOLI C,et al.Transcriptome analysis of cold acclimation in barley Albina and Xantha mutants[J].Plant Physiology,2006,141(1):257-270
[21] SONG Y P,CHEN Q Q,DONG C,et al.Transcriptome profiling reveals differential transcript abundance in response to chilling stress in Populus simonii[J].Plant Cell Reports,2013(32):1407-1425
[22] MARUYAMA K,URANO K,YOSHIWARA K,et al.Integrated analysis of the effects of cold and dehydration on rice metabolites,phytohormones,and gene transcripts[J].Plant Physiology,2014,164(4):1759-1771
[23] CHEN J R,LYU J J,LIU R,et al.DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M.truncatula and China rose (Rosa chinensis Jacq.)[J].Plant Growth Regulation,2010(60):199-211
[24] WANG C,GAO C Q,WANG L Q,et al.Comprehensive transcriptional profiling of NaHCO₃-stressed Tamarix hispida roots reveals networks of responsive genes[J].Plant Molecular Biology,2014(84):145-157
[25] POSTNIKOVA O A,SHAO J,NEMCHINOV L G.Analysis of the alfalfa root transcriptome in response to salinity stress[J].Plant and Cell Physiology,2013,54(7):1041-1055
[26] ZHANG J Y,CARVALHO M H,TORRES-JEREZ I,et al.Global reprogramming of transcription and metabolism in Medicago truncatula during progressive drought and after rewatering[J].Plant,Cell&Environment,2014(37):2553-2576
[27] CHEN H,ZENG Y,YANG Y,et al.Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa[J].Nature Communications,2020(11):2494
[28] XU H,LI Z,TONG Z,et al.Metabolomic analyses reveal substances that contribute to the increased freezing tolerance of alfalfa (Medicago sativa L.) after continuous water deficit[J].BMC Plant Biology,2020(20):15
[29] SONG L,JIANG L,CHEN Y,et al.Deep-sequencing transcriptome analysis of field-grown Medicago sativa L.crown buds acclimated to freezing stress[J].Functional&Integrative Genomics,2016,16(5):495-511
[30] KAZAN K.Diverse roles of jasmonates and ethylene in abiotic stress tolerance[J].Trends in Plant Science,2015,20(4):219-229
[31] VIERSTRA R D.Ubiquitin,a key component in the degradation of plant proteins[J].Physiologia Plantarum,1987,70(1):103-106
[32] ZHOU S,SUN X,YIN S,et al.The role of the F-box gene TaFBA1 from wheat (Triticum aestivum L.) in drought tolerance[J].Plant Physiology and Biochemistry,2014(84):213-223
[33] BU Q,LYU T,SHEN H,et al.Regulation of drought tolerance by the F-box protein MAX2 in Arabidopsis[J].Plant Physiology,2014,164(1):424-439
[34] JIAN B S,YAN X W,HAI B L,et al.The F-box family genes as key elements in response to salt,heavy mental,and drought stresses in Medicago truncatula[J].Functional&Integrative Genomics,2015,15(4):1-13
[35] ZHANG C K,LANG P,DANE F,et al.Cold acclimation induced genes of trifoliate orange (Poncirus trifoliata)[J].Plant Cell Reports,2005,23(10):764-769
[36] FONSECA S,CHICO J M,SOLANO R.The jasmonate pathway:the ligand,the receptor and the core signalling module[J].Current Opinion In Plant Biology,2009,12(5):539-547
[37] LYZENGA W J,STONE S L.Abiotic stress tolerance mediated by protein ubiquitination[J].Journal of Experimental Botany,2012,63(2):599-616
[38] YAN Y S,CHEN X Y,YANG K,et al.Overexpression of an F-box protein gene reduces abiotic stress tolerance and promotes root growth in rice[J].Molecular Plant,2011,4(1):190-197
[39] ZHOU S M,KONG X Z,KANG H H,et al.The involvement of wheat F-box protein gene TaFBA1 in the oxidative stress tolerance of plants[J].PLoS One,2015,10(4):e0122117
[40] PANJTANDOUST M,WOLYN D J.Freezing tolerance attributes during spring deacclimation for three asparagus cultivars with varying adaptation to Southern Ontario[J].Journal of the American Society for Horticultural Science,2016,141(1):22-33
[41] KALENGAMALIRO N E,GANA J A,CUNNINGHAM S M,et al.Mechanisms regulating differential freezing tolerance of suspension cell cultures derived from contrasting alfalfa genotypes[J].Plant Cell,Tissue and Organ Culture,2000(61):143-151
[42] XIN Z,BROWSE J.Cold comfort farm:the acclimation of plants to freezing temperatures[J].Plant,Cell&Environment,2000,23(9):893-902
[43] 陶雅.22个国内外苜蓿品种抗寒性评价[D].北京:中国农业科学院,2008:8-10
[44] CASTONGUAY Y,BERTRAND A,MICHAUD R,et al.Cold-induced biochemical and molecular changes in alfalfa populations selectively improved for freezing tolerance[J].Crop Science,2011,51(5):21-32
[45] SENGUPTA S,MUKHERJEE S,BASAK P,et al.Significance of galactinol and raffinose family oligosaccharide synthesis in plants[J].Frontiers in Plant Science,2015(6):656
[46] KAPLAN F,DONG Y S,GUY C L.Roles of β-amylase and starch breakdown during temperature stress[J].Physiologia Plantarum,2010,126(1):120-128
[47] 段志坤,秦晓惠,朱晓红,等.解析植物冷信号转导途径:植物如何感知低温[J].植物学报,2018,53(2):149-153
[48] LYONS J M.Chilling Injury in Plants[J].Annual Review of Plant Physiology,1973(24):445-466
[49] ERIC R.Moellering,Bagyalakshmi Muthan,Benning C.Freezing Tolerance in Plants Requires Lipid Remodeling at the Outer Chloroplast Membrane[J].Science,2010,330(6001):226-228
[50] GRENIER G,WILLEMOT C.Lipid changes in roots of frost hardy and less hardy alfalfa varieties under hardening conditions[J].Cryobiology,1974,11(4):324-331
[51] 孙明雪,张玉霞,丛百明,等.不同苜蓿的抗寒性差异及其与糖类物质含量的相关性研究[J].草地学报,2021,29(2):303-308
[52] SWIDERSKI A,MURAS P,KOLOCZEK H.Flavonoid composition in frost-resistant Rhododendron cultivars grown in Poland[J].Scientia Horticulturae,2004,100(4):139-151
[53] FüRTAUER L,WEISZMANN J,WECKWERTH W,et al.Dynamics of Plant Metabolism during Cold Acclimation[J].International Jounal of Molecular Sciences,2019,20(21):5411

Analyzing Cold Acclimation Process of Alfalfa Based on KEGG Pathway

基于KEGG通路分析紫花苜蓿的冷适应过程

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Jun, DING Xi-peng, CHEN Zhi-jian, MA Xiang-li, BI Yu-fen, GUO Feng-gen. Analysis of Differentially Expressed Genes in Pigeonpea [Cajanus cajan (L.) Millsp.] Responses to Cold Stress [J]. Acta Agrestia Sinica, 2022, 30(7): 1701-1711.
[2]	KAN Hai-ming, PANG Zhuo, CHEN Chao, ZOU Jun-liang, ZHANG Guo-fang, WU Ju-ying. Changes in Soil Microbial Communities Following the Vegetation Restoration of Degraded Sandy Grassland in Beijing [J]. Acta Agrestia Sinica, 2022, 30(6): 1350-1358.
[3]	LI Li-ping, LI Xu-lu, ZHANG Xiao-jie, GU Li-li, ZHANG Bo. Cloning and Expression Analysis of atpA Gene in Medicago sativa [J]. Acta Agrestia Sinica, 2022, 30(6): 1413-1421.
[4]	SUN Hao-nan, LI Rong, LI Ming-yang, ZHENG Yan, LIU Zhi-jie, ZHANG Cun-shi, LIU Dong-yun, LI He. Development of EST-SSR Markers based on Transcriptome and Analysis of Genetic Relationship of Cultivated Coreopsis [J]. Acta Agrestia Sinica, 2022, 30(6): 1430-1440.
[5]	HE Feng, DAI Xian-lin, WEI Bao, MA Xiao-ying, LI Zhen-song, YANG Fan, TONG Zong-yong, WANG Yu, LI Xiang-lin. Effects of Paddock Location and Grazing Stage on Sheep Defoliating Behavior in Alfalfa Mixture Grassland [J]. Acta Agrestia Sinica, 2022, 30(6): 1485-1490.
[6]	ZHANG Bo, KE Wen-can, LI Fu-hou, GUO Xu-sheng. Effects of Hay Preservatives on Different Sizes of Alfalfa Hay Bales [J]. Acta Agrestia Sinica, 2022, 30(6): 1569-1575.
[7]	WEI Jiang-wen, LIU Lei, WANG Sen-shan, CHEN Li-xia, JIANG Ming-jun, WANG Geng-hao, XU Heng-hao. Effects of Resistant and Susceptible Alfalfa Varieties on Adaptability and Enzyme Activity of Pea Aphid [J]. Acta Agrestia Sinica, 2022, 30(5): 1171-1177.
[8]	SUN Yan-liang, LIU Xuan-shuai, LI Sheng-yi, ZHAO Jun-wei, MA Chun-hui, ZHANG Qian-bing. Comprehensive Evaluation of Production Performance with different Fall Dormancy Rates of Alfalfa in Shihezi,Xinjiang [J]. Acta Agrestia Sinica, 2022, 30(5): 1227-1236.
[9]	ZAN Kan-zhuo, LIU Cong, ZHANG Yan-yan, Ashanjiang·Yiminijiang, BAI Jie, LONG Ming-xiu, WANG Ke-zhen, QU Yang, HE Shu-bin. Study on Soil Physical and Chemical Properties of Alfalfa,Wheat and Maize Rhizosphere in Guanzhong Area [J]. Acta Agrestia Sinica, 2022, 30(5): 1246-1252.
[10]	ZHANG Hai-xuan, HUO Wen-jie, CHEN Lei, LIU Qiang, ZHANG Shuan-lin, WANG Cong, XU Qing-fang, GUO Gang. Effects of Additives on the Fermentation Quality and the Absorption of Amino Acids in the Small Intestine of Alfalfa Silage [J]. Acta Agrestia Sinica, 2022, 30(5): 1302-1309.
[11]	WANG Jing, LIU Xiao-jing, TONG Chang-chun, WANG Xue. Study on the Difference of Nitrogen Efficiency of Alfalfa and its Influencing Factors [J]. Acta Agrestia Sinica, 2022, 30(4): 889-900.
[12]	LIU Dong-xia, YANG Li, SONG Lian-zhao, YANG Zhi-min, WANG Fang, LIU Gui-he. Comparative Study on Pathogenicity of Three Fusarium Strains to Different Alfalfa Varieties [J]. Acta Agrestia Sinica, 2022, 30(4): 909-918.
[13]	SUN Wan-bin, MA Hui-ling, ZHOU Qing-ping, CHEN You-jun, ZHANG Wei, ZHANG Jun-chao, LIU Wen-hui. Root Physiology and Characteristics of Different Alfalfa Varieties During Overwintering in Alpine Sub-humid Area [J]. Acta Agrestia Sinica, 2022, 30(4): 919-930.
[14]	ZAN Kan-zhuo, Ashanjiang·Yiminijiang, LIU Cong, TAN Kai, BAI Jie, HE Shu-bin, WANG Ke-zhen, QU Yang, LONG Ming-xiu. Physicochemical Properties and Stoichiometric Characteristics of Rhizosphere and Bulk Soils of Alfalfa of Different Fall Dormancy Types [J]. Acta Agrestia Sinica, 2022, 30(4): 957-965.
[15]	ZHANG Hao-yang, LIU Mei-jun, WEI dan-dan, MIAO Yu, ZHANG Li-jia. Comparative Study on Leaf Photosynthetic Activity of Different Fall Dormancy Alfalfa Before and After Winter [J]. Acta Agrestia Sinica, 2022, 30(4): 983-991.