Screening and Identification of Phosphate-Solubilizing Bacteria in Plant Rhizosphere of Alpine Meadow and Their Effects on Phosphate-Solubilizing and Plant Growth Promotion

doi:10.11733/j.issn.1007-0435.2022.11.031

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (11): 3132-3139.DOI: 10.11733/j.issn.1007-0435.2022.11.031

Screening and Identification of Phosphate-Solubilizing Bacteria in Plant Rhizosphere of Alpine Meadow and Their Effects on Phosphate-Solubilizing and Plant Growth Promotion

LIN Bao-jun¹, YANG Wen-quan², ZHAO Shuai¹, Chai Gang-ning¹, Yu Yang-hua¹, WU Yan-ru¹, HAN Xian-zhong³, LI Xi-lai⁴, KOU Jian-cun¹

1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
3. Menyuan County Grassland Station, Menyuan, Qinghai Province 810300, China;
4. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China

Received:2022-05-12 Revised:2022-07-18 Published:2022-12-02

高寒草甸植物根际溶磷菌的筛选鉴定及其溶磷与促生效果

蔺宝珺¹, 杨文权², 赵帅¹, 柴港宁¹, 鱼杨华¹, 武燕茹¹, 韩显忠³, 李希来⁴, 寇建村¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西北农林科技大学生命科学学院, 陕西杨凌 712100;
3. 门源县草原站, 青海门源 810300;
4. 青海大学农牧学院, 青海西宁 810016

通讯作者: 寇建村,E-mail:jiancun02@163.com
作者简介:蔺宝珺(1997-)，女，汉族，甘肃陇西人，主要从事草地生态恢复学研究，E-mail:lbj2020@nwafu.edu.com
基金资助:
国家自然科学基金项目(U21A20191)；2021年第一批林业草原生态保护恢复资金自然资源调查监测项目(QHXH-2021-018)；中央财政林草科技推广示范项目(青[2021]TG01号)；青海省财政林业改革发展资金技术推广项目([2021]001号)资助

Abstract

Abstract: In order to obtain the excellent phosphate-solubilizing bacteria resources in the rhizosphere soil of the alpine meadow,four phosphate-solubilizing bacteria were screened from the rhizosphere soil of an alpine meadow in Qinghai province and identified by 16S rRNA gene analysis. Through the anti-molybdenum antimony colorimetry and pot experiment the effects of dissolving phosphorus and promoting growth were studied. The results showed that the four strains were Pseudomonas. They all could form prominent phosphate solubilizing circles. When cultured in inorganic phosphate liquid medium for 7 days,the phosphorus the four strains released was 156.17~511.33 μg·mL^-1. Various organic acids were secreted by the four strains during phosphate solubilization,and the total organic acid amount ranged from 522.36 to 986.69 mg·L^-1. Pot experiments showed that four strains could significantly increase the plant height and aboveground dry weight of Elymus dahuricus,and promote the growth of Elymus dahuricus. Strains MXSC5,MXSC6 and MQC13 could increase the contents of total N and total P in plants,and the four strains also all had a positive effect on available N and available P content of soil. The results help to investigate further the role of phosphorus-solubilizing bacteria in the promotion of plant growth and the development and utilization of microbial fertilizers.

Key words: Alpine meadow, Phosphorus-solubilizing bacteria, Identification, Phosphorus solubilization capacity, Promoting effect

摘要： 为获得高寒草甸根际土中优良溶磷菌资源，本研究从青海省高寒草甸根际土中筛选了4株溶磷菌，结合16S rRNA基因分析法确定其分类地位，并通过钼锑抗比色法和盆栽试验进行了溶磷与促生效果的研究。结果显示：4株溶磷菌均为假单胞菌属(Pseudomonas sp.)，均可形成明显的溶磷圈。在无机磷液体培养基中培养7d后，4株菌株的磷增量在156.17~511.33μg·mL^-1之间；溶磷过程中4株菌均分泌多种有机酸，总有机酸量在522.36~986.69mg·L^-1之间；盆栽试验表明4株菌均能显著增加披碱草(Elymus dahuricus Turcz.)株高和地上部干重；菌株MXSC5，MXSC6和MQC13可使植株全氮、全磷含量增加，且4株菌株对土壤速效氮、速效磷含量也有正向影响。本研究结果将有助于进一步探究溶磷菌对植物的促生作用及微生物菌肥的开发利用。

关键词: 高寒草甸, 溶磷菌, 鉴定, 溶磷能力, 促生效果

CLC Number:

S154.39

LIN Bao-jun, YANG Wen-quan, ZHAO Shuai, Chai Gang-ning, Yu Yang-hua, WU Yan-ru, HAN Xian-zhong, LI Xi-lai, KOU Jian-cun. Screening and Identification of Phosphate-Solubilizing Bacteria in Plant Rhizosphere of Alpine Meadow and Their Effects on Phosphate-Solubilizing and Plant Growth Promotion[J]. Acta Agrestia Sinica, 2022, 30(11): 3132-3139.

蔺宝珺, 杨文权, 赵帅, 柴港宁, 鱼杨华, 武燕茹, 韩显忠, 李希来, 寇建村. 高寒草甸植物根际溶磷菌的筛选鉴定及其溶磷与促生效果[J]. 草地学报, 2022, 30(11): 3132-3139.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.11.031

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I11/3132

References

[1] 史发超,殷中伟,江红梅,等. 一株溶磷真菌筛选鉴定及其溶磷促生效果[J]. 微生物学报,2014,54(11):1333-1343
[2] 薛应钰,叶巍,杨树,等. 一株溶磷菌的分离鉴定及溶磷促生作用[J]. 干旱地区农业研究,2019,37(4):253-262
[3] KHAN M S,ZAIDI A,WANI P A. Role of Phosphate-solubilizing Microorganisms in Sustainable Agriculture-a Review[J]. Agronomy for Sustainable Development,2007,27(1):29-43
[4] 李海云,孔维宝,达文燕,等. 土壤溶磷微生物研究进展[J]. 生物学通报,2013,48(7):1-5
[5] 李栎,王光军,周国新,等. 会同桢楠人工幼林土壤C:N:P生态化学计量的时空特征[J]. 中南林业科技大学学报,2016,36(2):6
[6] 张巍,冯玉杰,胡纯国,等. 耐盐碱解磷菌的分离鉴定及解磷能力研究[J]. 土壤通报,2009,40(3):572-575
[7] RAWAT P,DAS S,SHANKHDHAR D,et al. Phosphate-solubilizing Microorganisms:Mechanism and Their Role in Phosphate Solubilization and Uptake[J]. Journal of Soil Science and Plant Nutrition,2020,21(1):49-68
[8] 冯月红,姚拓,龙瑞军. 土壤解磷菌研究进展[J]. 草原与草坪,2003(1):3-7
[9] MENDES G D O,MURTA H M,VALADARES R V,et al. Oxalic Acid is More Efficient than Sulfuric Acid for Rock Phosphate Solubilization[J]. Minerals Engineering,2020(155):106458
[10] 郑淇元,谢意太,卞海洋,等. 溶磷菌肥对红壤磷组分及土壤肥力的影响[J]. 江西农业大学学报,2022,44(1):233-244
[11] KAFLE A,COPE K R,RATHS R,et al. Harnessing Soil Microbes to Improve Plant Phosphate Efficiency in Cropping Systems[J]. Agronomy,2019,9(3):127
[12] 汪金华,杨腊英,黄俊生,等. 西瓜根际土壤解磷芽孢杆菌的筛选及其解磷特性研究[J]. 海南大学学报(自然科学版),2021,39(2):146-152
[13] 侯娇,李莎. 干旱区1株新欧文氏属菌的分离及溶磷特性[J]. 环境科学与技术,2021,44(2):19-24
[14] 范延辉,王君,尚帅,等. 两株根际真菌的耐盐、溶磷、促生效果及其分类鉴定[J]. 土壤通报,2022,53(1):127-134
[15] 李美. 溶磷菌筛选及对小麦、玉米促生长作用研究[D]. 兰州:兰州理工大学,2021:2
[16] VIRUEL E,ERAZZU L E,MARTINEZ C L,et al. Inoculation of Maize with Phosphate Solubilizing Bacteria:Effect on Plant Growth and Yield[J]. Journal of Soil Science and Plant Nutrition,2014,14(4):819-831
[17] REZAKHANI L,MOTESHAREZADEH B,TEHRANI M M,et al. Phosphate-solubilizing Bacteria and Silicon Synergistically Augment Phosphorus (P) Uptake by Wheat (Triticum aestivum L.) Plant Fertilized with Soluble or Insoluble P Source[J]. Ecotoxicology and Environmental Safety,2019(173):504-513
[18] 赵艳利. 亚热带水稻土溶磷菌分离鉴定及其溶磷效果和机制研究[D]. 赣州:江西理工大学,2021:9
[19] 杨婉秋,敬洁,朱灵,等. 川西北高寒草甸植物根际促生菌筛选及其特性研究[J]. 草地学报,2021,29(6):1174-1182
[20] 刘晓婷,姚拓.高寒草地耐低温植物根际促生菌的筛选鉴定及特性研究[J]. 草业学报,2022,31(8):178-187
[21] 张英. 西藏阿里高寒草原四种牧草根际促生菌资源筛选及促生机理研究[D]. 兰州:甘肃农业大学,2013:4-25
[22] 赵树栋,李建宏. 高原早熟禾根际促生菌分离筛选及特性研究[J]. 草地学报,2021,29(9):1885-1891
[23] 沈萍,陈向东. 微生物学实验[M]. 北京:高等教育出版社,2000:14-15
[24] 马文文,姚拓,蒲小鹏,等. 东祁连山7种禾草根际溶磷菌筛选及其溶磷特性[J]. 草业科学,2015,32(4):515-523
[25] 王舒,张林平,张扬,等.红壤区油茶根际解磷细菌的筛选、鉴定及其解磷能力[J]. 林业科学研究,2015,28(3):409-416
[26] 管国强,李倩,季蓉蓉,等. 1株溶磷细菌P0417的溶磷机制[J]. 江苏农业科学,2015,43(10):432-435
[27] 程宇阳. 苹果园白三叶根际土溶磷菌的分离、鉴定及其特性研究[D]. 杨凌:西北农林科技大学,2020:26-47
[28] 霍云倩. 木霉生物有机肥对羊草生长和土壤微生物区系的影响[D]. 南京:南京农业大学,2018:28
[29] 鲍士旦. 土壤农化分析[M].第3版.北京:中国农业出版社,2000:30-270
[30] 胡山,牛世全,龙洋,等. 河西走廊盐碱土壤中一株高效溶磷菌的鉴定及条件优化[J]. 微生物学通报,2017,44(2):358-365
[31] ADHIKARI P,JAIN R,SHARMA A,et al. Plant Growth Promotion at Low Temperature by Phosphate-solubilizing Pseudomonas Spp. Isolated from High-altitude Himalayan Soil[J]. Microbial Ecology,2021,82(3):677-687
[32] EI-DEEN R,El-AZEEM S,EIWAHAB A,et al. Effects of Phosphate Solubilizing Microorganisms on Wheat Yield and Phosphatase Activity[J]. Egyptian Journal of Microbiology,2020,55(1):71-86
[33] KHAN M S,ZAIDI A,AHMAD E. Mechanism of Phosphate Solubilization and Physiological Functions of Phosphate-solubilizing Microorganisms[M]. Berlin:Springer,2014:31-62
[34] 赵小蓉,林启美,李保国. 微生物溶解磷矿粉能力与pH及分泌有机酸的关系[J]. 微生物学杂志,2003(3):5-7
[35] 秦利均,杨永柱,杨星勇. 土壤溶磷微生物溶磷、解磷机制研究进展[J]. 生命科学研究,2019,23(1):59-64
[36] ANDERSSON K O,TIGHE M K,GUPPY C N,et al. Incremental Acidification Reveals Phosphorus Release Dynamics in Alkaline Vertic Soils[J]. Geoderma,2015(259):35-44
[37] RASHID M,KHALIL S,AYUB N,et al. Organic Acids Production and Phosphate Solubilization by Phosphate Solubilizing Microorganisms (PSM) Under in Vitro Conditions[J]. Pakistan Journal of Biological Sciences,2004,7(2):187-196
[38] 刘文干,何园球,张坤,等. 一株红壤溶磷菌的分离、鉴定及溶磷特性[J]. 微生物学报,2012,52(3):326-333
[39] Illmer P,Schinner F. Solubilization of Inorganic Calcium Phosphates-solubilization Mechanisms[J]. Soil Biology and Biochemistry,1995,27(3):257-263
[40] 赵小蓉,林启美,李保国. 溶磷菌对4种难溶性磷酸盐溶解能力的初步研究[J]. 微生物学报,2002(2):236-241
[41] KANG S C,CHOI M C. Solid Culture of Phosphate-solubilizing Fungus,Penicillium sp. PS-113[J]. Microbiology and Biotechnology Letters,1999,27(1):1-7
[42] 朱培淼,杨兴明,徐阳春,等. 高效解磷细菌的筛选及其对玉米苗期生长的促进作用[J]. 应用生态学报,2007(1):107-112
[43] WU F,LI J,CHEN Y,et al. Effects of Phosphate Solubilizing Bacteria on the Growth,Photosynthesis,and Nutrient Uptake of Camellia Oleifera Abel[J]. Forests,2019,10(4):348
[44] KUREK E,OZIMEK E,SOBICZEWSKI P,et al. Effect of Pseudomonas Luteola on Mobilization of Phosphorus and Growth of Young Apple Trees (Ligol)-Pot Experiment[J]. Scientia Horticulturae,2013(164):270-276
[45] 王誉瑶,韦中,徐阳春,等. 溶磷菌株组合的溶磷效应及对玉米生长的影响[J]. 植物营养与肥料学报,2017,23(1):262-268
[46] 池景良,郝敏,王志学,等. 解磷微生物研究及应用进展[J]. 微生物学杂志,2021,41(1):1-7
[47] YU X,LIU X,ZHU T H,et al. Isolation and Characterization of Phosphate-solubilizing Bacteria from Walnut and their Effect on Growth and Phosphorus Mobilization[J]. Biology and Fertility of Soils,2011,47(4):437-446
[48] LIU J,QI W,LI Q,et al. Exogenous Phosphorus-solubilizing Bacteria Changed the Rhizosphere Microbial Community 257 Indirectly[J]. Biotech,2020,10(4):1-11

Screening and Identification of Phosphate-Solubilizing Bacteria in Plant Rhizosphere of Alpine Meadow and Their Effects on Phosphate-Solubilizing and Plant Growth Promotion

高寒草甸植物根际溶磷菌的筛选鉴定及其溶磷与促生效果

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yu-qin, SONG Mei-ling, ZHOU Rui, WANG Hong-sheng, MA Yu-shou. Characteristics of Plant Community Structure of Different Ligularia virgaurea Density Patches in Alpine Meadow [J]. Acta Agrestia Sinica, 2022, 30(9): 2264-2272.
[2]	MA Kai-kai, XU Chang-lin, LI Ying, WEI Kong-tao, BAI Mei-mei, LIU Yuan-yuan, YU Xiao-jun. Effects of Different Rest Grazing Periods on Soil Seed Bank in Cold Season Pasture of Alpine Meadow [J]. Acta Agrestia Sinica, 2022, 30(8): 2100-2107.
[3]	CHAI Yu, LI Xi-lai, YU Jin-feng, YIXI Droma, SONG Xian, MA Pan-pan, DUAN Cheng-wei, XU Wen-yin. Effects of Different Applications of Organic Fertilizer in Degraded Alpine Meadow on Soil Aggregates and Organic Carbon in the Source Zone of Yellow River [J]. Acta Agrestia Sinica, 2022, 30(7): 1613-1620.
[4]	SONG Zhi-ping, WANG Xiao-li, WANG Yan-long, ZHOU Xuan-bo, XIE Le-le. Response of Ecological Niche of Alpine Meadow Population to Rest Grazing Time During Green Period in Three-River Source Region [J]. Acta Agrestia Sinica, 2022, 30(7): 1651-1658.
[5]	WEN Tong, LIU Xiao-ni, JI Tong, YANG Dong-yang, QIAO Huan-huan, JIANG Jia-chang, PAN Dong-rong. Studying on Plant Classification and Recognition Method for Three-River Source Alpine Grassland Plant Based on Vegetation Index [J]. Acta Agrestia Sinica, 2022, 30(7): 1811-1818.
[6]	YANG Ze-yi, ZHANG Yu-lin, YANG Han-jun, CHE Bing, ZHANG Fan-fan, MA Chun-hui. Isolation and Identification of Dominant Lactic Acid Bacteria in Natural Silage of Grape Branch and Leaf [J]. Acta Agrestia Sinica, 2022, 30(7): 1918-1924.
[7]	ZHANG Chun-hui, MA Zhen, REN Yan-mei, WU Lin, YAO Bu-qing, HUANG Xiao-tao, MA Li, GUO Jing, ZHOU Hua-kun. The Study on Drought Tolerance of Six Forbs during Seed Germination in an Alpine Meadow of Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2022, 30(5): 1159-1164.
[8]	ZHANG Cai-jun, WANG Xiao-yan, LIU Qian-qian, AN Kang, PU Qiang-sheng, WANG Zhi-cheng, SU Jun-hu. Effects of Human Activities on the Feeding Habits of Himalayan Marmots in Gannan Meadow [J]. Acta Agrestia Sinica, 2022, 30(5): 1219-1226.
[9]	ZHANG Yu-peng, WU Xiao-tian, LI Xi-lai, XU Wen-yin, DONG Xin-pu, WANG Yuan, ZHANG Hui. Responses and Distributions of Degraded Alpine Meadow on the Physical and Chemical Properties of Soil in River Basin Units in the Source Region of Yellow River [J]. Acta Agrestia Sinica, 2022, 30(3): 503-512.
[10]	FANG Hao-tian, WENG Bai-sha, CHANG Wen-juan, YANG Yu-heng, GONG Xiao-yan. Characteristics of Root Distribution in Alpine Meadows and the Relationship with Soil Temperature and Moisture [J]. Acta Agrestia Sinica, 2022, 30(3): 612-621.
[11]	WANG Xiao-yan, ZHANG Cai-jun, PU Qiang-sheng, AN Kang, ZHANG Qian, YANG Jing, SUN Xiao-mei, SU Jun-hu. Effects of Artificial Grassland Construction on Grassland Productivity and Soil Physicochemical Properties in Alpine Meadow of Gannan [J]. Acta Agrestia Sinica, 2022, 30(2): 288-296.
[12]	WANG Jing, LIU Xiong-zhou, JIN Guan-fang, DORJI Tsechoe, CIREN Qu-zong, YIN Shu-xia, YANG Zhi-yong. Effects of Seasonal Asymmetric Simulated Warming on Community Characteristics of Alpine Meadows on the Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2022, 30(11): 3056-3062.
[13]	HUO Jia-juan, ZHU Jue-fei, SONG Ming-hua, LI Yi-kang, XU Xing-liang, ZHOU Hua-kun. Characteristics of Vegetation Patches in the Process of Degradation Succession in an Alpine Meadow on Tibet Plateau [J]. Acta Agrestia Sinica, 2022, 30(11): 3113-3118.
[14]	YE Qiong-dan, REN Fei, LI Yong-hui, ZHANG Zi-yang, FAN Na, YE Jun, LI Xi-lai, LI Lan-ping. Effects of Different Nutrient Additions on Functional Traits of Alpine Plant [J]. Acta Agrestia Sinica, 2022, 30(10): 2737-2744.
[15]	ZHANG Yu-lin, YANG Han-jun, LI Chao-cheng, ZHANG Fan-fan, MA Chun-hui. Isolation and Identification of Lactic Acid Bacterium Strain From Broussonetia Papyrifera Silage [J]. Acta Agrestia Sinica, 2022, 30(1): 38-45.