Identification of Wild Elymus sibiricus Germplasm Resources and Analysis of Variation of Dorsal Hairs in Basal Leaf Sheath

doi:10.11733/j.issn.1007-0435.2023.04.011

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (4): 1026-1035.DOI: 10.11733/j.issn.1007-0435.2023.04.011

Identification of Wild Elymus sibiricus Germplasm Resources and Analysis of Variation of Dorsal Hairs in Basal Leaf Sheath

LI Ming-feng^1,2, LI Xin-rui^2,3, LI Ying-zhu², CHEN Li-li², WANG Yan¹, LI Chun-yan¹, CHANG Dan², LEI Xiong², YAN Li-jun², YOU Ming-hong², JI Xiao-fei², ZHANG Chang-bing², WU Qi², GOU Wen-long², LI Da-xu², CHEN Shi-yong¹, BAI Shi-qie², YAN Jia-jun²

1. Southwest Minzu University, Chengdu, Sichuan Province 610041, China;
2. Sichuan Academy of Grassland Sciences, Chengdu, Sichuan Province 611743, China;
3. Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China

Received:2022-11-03 Revised:2023-01-21 Published:2023-04-28

野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析

李明峰^1,2, 李欣瑞^2,3, 李英主², 陈丽丽², 王艳¹, 李春艳¹, 常丹², 雷雄², 闫利军², 游明鸿², 季晓菲², 张昌兵², 吴婍², 苟文龙², 李达旭², 陈仕勇¹, 白史且², 鄢家俊²

1. 西南民族大学, 四川成都 610041;
2. 四川省草原科学研究院, 四川成都 611743;
3. 四川农业大学, 四川成都 611130

通讯作者: 白史且,E-mail:baishiqie@126.com;鄢家俊,E-mail:51037529@qq.com
作者简介:李明峰(1997-),男,汉族,四川达州人,硕士研究生,主要从事草种质资源开发与利用研究,E-mail：lmf2464@126.com;李欣瑞(1996-),男,汉族,内蒙古呼伦贝尔人,博士研究生,主要从事草种质资源开发与利用,E-mail：810106355@qq.com
基金资助:
四川省科技创新人才项目——青藏高原乡土草种老芒麦种质资源重要性状精准鉴定与育种应用(2023JDRC0040)；四川省林草科技创新团队专项资金(LCTD2023CZ01)；四川省“十四五”饲草育种攻关—高寒牧区多用途牧草新品种选育及配套技术研究(2021YFYZ0013-2)；国家种质资源红原观测实验站(NAES020GR04)；野生植物四川省科技资源共享服务平台资助

Abstract

Abstract: In order to make better use of the wild resources of Elymus sibiricus germplasm,in this study the 1 723 materials of wild Elymus species resources from the Qinghai-Tibet Plateau,Northwest of China,North China and Northeast of China and some foreign regions were collected,from which,total of 990 wild E. sibiricus germplasm were identified by phenotypic characteristics and the Flow Cytometry detection. The results showed that the DNA content of E. sibiricus was between 5.86~7.30 Gb with average of 6.66Gb. The DNA content of Elymus nutans Griseb was between 9.50~10.36 Gb with average of 9.97Gb. It was found out that dorsal hair appeared in the basal leaf sheath of some wild E. sibiricus materials from Northwest of China,North China and Northeast of China,which was different from the description of the morphological characteristics of E. sibiricus in Chinese Flora,which was positively correlated with longitude and latitude (P < 0.01),and negatively correlated with altitude,annual mean temperature and annual mean rainfall (P<0.01),indicating that there was an inevitable relationship between the existence of the dorsal hair in the basal leaf sheath of E. sibiricus and its environmental factors and which possibly was one of the adaptive mechanisms of E. sibiricus germplasm to its environment. The results of this study provided a new basis for the morphological identification of E. sibiricus,and for the exploring E. sibiricus germplasm resources and breeding application.

Key words: Elymus sibiricus, Phenotypic identification, Flow cytometry, Dorsal hair in the basal leaf sheath

摘要： 为更好地利用老芒麦(Elymus sibiricus L.)野生资源，本研究从我国青藏高原、西北、华北、东北地区以及国外部分地区共采集了1 723份野生披碱草属种质资源，通过表型特征观测和流式细胞仪检测，共鉴定出了990份野生老芒麦种质，246份垂穗披碱草种质，并测得老芒麦的DNA含量在5.86~7.30 Gb之间，平均DNA含量为6.66 Gb；垂穗披碱草材料的DNA含量在9.50~10.36 Gb之间，平均DNA含量为9.97 Gb。研究结果显示，在西北、华北以及东北地区的部分野生老芒麦材料在苗期基部叶鞘出现了绒毛，分析发现该表型出现的概率与经度和纬度呈极显著正相关关系(P<0.01)，与海拔、年年均气温以及年平均降雨量呈极显著负相关关系(P<0.01)。表明老芒麦苗期基部叶鞘绒毛的有无与环境因子之间存在着必然的联系，可能是老芒麦种质应对不同环境类型的适应性机制之一。本研究为老芒麦的形态鉴定提供了新的依据，为老芒麦种质资源挖掘和育种应用提供了材料基础。

关键词: 老芒麦, 表型鉴定, 流式细胞术, 基部叶鞘绒毛

CLC Number:

S543.9

LI Ming-feng, LI Xin-rui, LI Ying-zhu, CHEN Li-li, WANG Yan, LI Chun-yan, CHANG Dan, LEI Xiong, YAN Li-jun, YOU Ming-hong, JI Xiao-fei, ZHANG Chang-bing, WU Qi, GOU Wen-long, LI Da-xu, CHEN Shi-yong, BAI Shi-qie, YAN Jia-jun. Identification of Wild Elymus sibiricus Germplasm Resources and Analysis of Variation of Dorsal Hairs in Basal Leaf Sheath[J]. Acta Agrestia Sinica, 2023, 31(4): 1026-1035.

李明峰, 李欣瑞, 李英主, 陈丽丽, 王艳, 李春艳, 常丹, 雷雄, 闫利军, 游明鸿, 季晓菲, 张昌兵, 吴婍, 苟文龙, 李达旭, 陈仕勇, 白史且, 鄢家俊. 野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析[J]. 草地学报, 2023, 31(4): 1026-1035.

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References

[1] 王传旗,刘文辉,张永超,等. 野生老芒麦苗期耐旱性研究[J]. 草业学报,2021,30(8):127-136
[2] 吴瑞,刘文辉,张永超,等. 青藏高原地区不同老芒麦种质资源生产性能评价[J]. 植物遗传资源学报,2020,21(4):855-165
[3] 张荟荟,梁维维,张学洲,等. 新疆野生老芒麦种质资源形态及生长特性分析[J]. 草地学报,2021,29(4):701-708
[4] 李欣瑞,陈淑娴,鄢家俊,等. 老芒麦种质资源研究进展[J]. 草学,2021(1):6-17
[5] 吴瑞,刘文辉,张永超,等. 青藏高原老芒麦落粒性及农艺性状相关性研究[J]. 草业学报,2021,30(4):130-139
[6] 李瑶. 老芒麦种质资源表型多样性及落粒性研究[D]. 乌鲁木齐:新疆农业大学,2020:15-28
[7] 曾翰国,岳佳铭,张曙恒,等. 76份老芒麦种质资源的农艺性状综合评价[J]. 草地学报,2022,30(11):3046-3055
[8] 尹婷婷,谷丽丽,闫锋,等. 59份老芒麦种质资源的表型多样性分析[J]. 西南农业学报,2021,34(11):2307-2317
[9] 中国科学院中国植物志委员会. 中国植物志(第九卷.第三分册)[M]. 中国植物志.第九卷.第三分册,1987:3,9
[10] 张同林,晏国生. 披碱草属分类的研究及其存在的问题[J]. 草地学报,2012,20(2):207-212
[11] 王琴,邰丽华,哈斯巴根,等. 披碱草属七种植物核型及其亲缘关系的研究[J]. 内蒙古师范大学学报(自然科学汉文版),2013,42(2):192-200
[12] 郑慧敏,毛培胜,黄莺. 老芒麦染色体组型分析[J]. 草业学报,2015,24(8):225-230
[13] 谢小梅,许杨. 流式细胞术[J]. 中国生物工程杂志,2003,23(9):100-104
[14] ADAN A,ALIZADA G,KIRAZ Y,et al. Flow cytometry:basic principles and applications[J]. Critical Reviews in Biotechnology,2017,37(2),163-176
[15] 王荣邦,张秀海,吴忠义,等. 菊花染色体倍性鉴定研究[J]. 安徽农业科学,2010,38(23):12778-12780,12789
[16] 冯娇娇. 流式细胞术在植物上的应用[D]. 杨凌:西北农林科技大学,2017:10-41
[17] VRANA J,KUBALAKOVA M,SIMKOVA H,et al. Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.)[J]. Genetics,2000,156(4):531-536
[18] 许蕾,陈佩琳,冯光燕,等. 利用流式细胞仪鉴定鸭茅倍性[J]. 草业学报,2019,28(3):74-84
[19] YAN J J,ZHANG J B,SUN K Y,et al. Ploidy Level and DNA Content of Erianthus arundinaceus as Determined by Flow Cytometry and the Association with Biological Characteristics[J]. PloS One,2016,11(3):e0151948
[20] 钟米莎,刘琳,吴菲菲,等. 草地植物鉴定分类方法的现状与应用[J]. 中国草地学报,2020,42(6):157-164
[21] 陈林,宋丽. 流式细胞术的发展及在植物研究中的应用[J]. 生物工程学报,2023,39(2):472-487
[22] GALBRAITH D,LOUREIRO J,ANTONIADI I. Best practices in plant cytometry[J]. Cytometry Part A,2021,99(4):311-317
[23] 叶脉. 金属型植物酸模(Rumex acetosa L.)和鸭跖草(Commelina communis L.)的适应性进化[D]. 广东:中山大学,2009:7-8
[24] 侯曼曼. 基于系统发育和叶片功能性状的鄱阳湖区薹草属部分植物对生境的适应性进化[D]. 南昌:南昌大学,2017:1-3
[25] 袁庆华,张吉宇,张文淑,等. 披碱草和老芒麦野生居群生物多样性研究[J]. 草业学报,2003,12(5):44-49
[26] 韩梦丽. 青藏高原老芒麦(Elymus Sibiricus L.)系统发育和遗传分化[D]. 扬州:扬州大学,2022:29-49
[27] 鄢家俊,白史且,张昌兵,等. 青藏高原老芒麦野生种群生态特性与形态变异研究[J]. 中国草地学报,2010,32(4):49-57
[28] LIU Z H,WANG Q,WAN H S,et al. Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace'Baimaomai’[J]. Agronomy,2021,11(11):2237-2237
[29] CLAUSEN J,KECK D,HIESEY W M. Experimental studies on the nature of species. I. The effect of varied environments of Western North American plants[M].Washington DC:Carnegie Instistute of Was ington，1940:2-25
[30] EHLERINGER J. Biology and Chemistry of Plant Trichomes[M]. New York:Plenum Press,1984:88
[31] KONRAD W,BURKHARDT J,ENBER M,et al. Leaf pubescence as a possibility to increase water use efficiency by promoting condensation[J]. Ecohydrology,2014,8(3):480-492
[32] WOOD W,GORE J,CATCHOT A,et al. Effect of leaf pubescence on tarnished plant bug (Hemiptera:Miridae) ability to cause damage and yield loss in cotton[J]. Journal of Cotton Science,2017,21(2):122-127
[33] PRACHI P,VADIVELMURUGAN I,BAGAVATHIANNAN M V,et al. Impact of Combined Abiotic and Biotic Stresses on Plant Growth and Avenues for Crop Improvement by Exploiting Physio-morphological Traits[J]. Frontiers in Plant Science,2017,8:537
[34] DOBROVOLSKAYA O,PSHENICHNIKOVA T P,ARBUZOVA V S,et al. Molecular mapping of genes determining hairy leaf character in common wheat with respect to other species of the Triticeae[J]. Euphytica,2007,155(3):285-293
[35] 崔紫霞. 六倍体小黑麦材料创制与重要性状遗传研究[D]. 杨凌:西北农林科技大学,2018:18-20
[36] 闫天芳,苗彦军,王向涛,等. 干旱胁迫对西藏4份野生披碱草属牧草幼苗生理指标的影响[J]. 草地学报,2017,25(6):1333-1339
[37] 王传旗,刘文辉,张永超,等. 野生老芒麦苗期耐旱性品种筛选及鉴定[J]. 草业科学,2021,38(5):903-917
[38] 何子华,杨成行,王沛,等. 高寒地区6种禾本科牧草对低温胁迫的生理响应及耐寒性评价[J]. 草业科学,2021,38(10):2019-2028
[39] 赵慧,姚俊强,李新国,等. 新疆气候干湿变化特征分析[J]. 中山大学学报(自然科学版),2020,59(5):126-133
[40] 余迪,段丽君,温婷婷,等. 青藏高原雨季特征及其对气候增暖的响应[J]. 气象与环境学报,2021,37(2):12-18
[41] 唐亚平,张凯,张伟,等. 东北雨季降水集中度和集中期的变化特征[J]. 安徽农业科学,2010,38(2):806-811
[42] 王皓,李子忠. 农牧交错带老芒麦的优化灌溉及对产量的影响[J]. 农业工程学报,2008,24(12):6-11

Identification of Wild Elymus sibiricus Germplasm Resources and Analysis of Variation of Dorsal Hairs in Basal Leaf Sheath

野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析

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