Response of AsA-GSH Cycles to Exogenous H2O2 Priming in the Embryonic Cells and Mitochondria of Oat Seeds

doi:10.11733/j.issn.1007-0435.2023.04.015

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (4): 1064-1070.DOI: 10.11733/j.issn.1007-0435.2023.04.015

Response of AsA-GSH Cycles to Exogenous H₂O₂ Priming in the Embryonic Cells and Mitochondria of Oat Seeds

LI Hong-yu, WANG Ya-cong, XIA Fang-shan, WANG Bo, LI Yin-lin, ZHANG Jie-min

College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2022-11-08 Revised:2022-12-13 Published:2023-04-28

燕麦种胚细胞和线粒体AsA-GSH循环对外源H₂O₂引发的响应

李红玉, 王雅聪, 夏方山, 王勃, 李尹琳, 张杰敏

山西农业大学草业学院, 山西太谷 030801

通讯作者: 夏方山,E-mail:dqlxfs8583@163.com
作者简介:李红玉(1973-),女,汉族,山西太谷人,硕士,高级实验师,主要从事草种子科学研究,E-mail:lihongyu289@126.com
基金资助:
山西农业大学“十四五”生物育种工程项目(YZGC134)；国家自然基金项目(31702171)；山西省重点研发计划项目(201903D221091，202102140601006)；内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)资助

Abstract

Abstract: In this study,the changes in the antioxidant properties of ascorbic acid (AsA)-glutathione (GSH) cycles were explored as a response to exogenous H₂O₂ priming in the embryonic cells and mitochondria of oat (Avena sativa) seeds,which provided a theoretical basis for the long-term storage of plant seeds. Oat seeds were primed with 0,0.96,1.92,3.84 and 7.68 mol·L^-1 H₂O₂ for 12 h as the experimental materials,and the changes in lipid peroxidation and enzymatic and non-enzymatic antioxidants in AsA-GSH cycles were analyzed in both their embryonic cells and mitochondria. The results showed that the AsA and GSH contents were significantly decreased in the embryonic cells and mitochondria (P<0.05),and enzymatic activities of AsA-GSH cycles and contents of dehydroascorbate and oxidized glutathione were all with a pattern of increasing firstly and then decreasing. H₂O₂ and malondialdehyde contents were also significantly increased (P<0.05),and thus the germination percentage of oat seeds was reduced significantly (P<0.05). Embryonic cells and mitochondria showed similar results of changes of AsA-GSH cycles in response to the increase of H₂O₂ concentration,and the damage on their antioxidant capacity resulted in the decrease of seed germination under priming with high concentration (≥1.92 mol·L^-1) of exogenous H₂O₂. It might be caused largely by the decrease of AsA content and its continuous production.

Key words: Ascorbic acid-glutathione cycles, Mitochondria, Hydrogen peroxide, Oat, Seed priming

摘要： 本研究探讨了外源H₂O₂引发后，燕麦(Avena sativa)种胚细胞和线粒体抗坏血酸(Ascorbic acid，AsA)-谷胱甘肽(Glutathione，GSH)循环的抗氧化性能的变化规律，以期为植物种子的长期贮藏技术研究提供理论依据。试验以燕麦种子为材料，用浓度为0，0.96，1.92，3.84和7.68 mol·L^-1的H₂O₂引发12 h后，分析其种胚细胞和线粒体脂质过氧化水平、AsA-GSH循环中酶促和非酶促抗氧化物质的变化规律。结果表明：燕麦种胚细胞和线粒体内AsA和GSH含量会随外源H₂O₂浓度的增加而显著降低(P<0.05)，其AsA-GSH循环的酶活性、脱氢抗坏血酸和氧化型谷胱甘肽含量均呈先升后降的趋势，其H₂O₂和丙二醛含量显著升高(P<0.05)，燕麦种子发芽率则显著降低(P<0.05)。燕麦种胚细胞和线粒体AsA-GSH循环的抗氧化功能变化对H₂O₂浓度升高的响应具有协同性，其抗氧化能力下降导致高浓度(≥1.92 mol·L^-1)外源H₂O₂引发下燕麦种子发芽率降低，而其AsA含量及再生能力的降低是造成这一现象的主要原因。

关键词: 抗坏血酸-谷胱甘肽循环, 线粒体, 过氧化氢, 燕麦, 种子引发

CLC Number:

S512.6

LI Hong-yu, WANG Ya-cong, XIA Fang-shan, WANG Bo, LI Yin-lin, ZHANG Jie-min. Response of AsA-GSH Cycles to Exogenous H₂O₂ Priming in the Embryonic Cells and Mitochondria of Oat Seeds[J]. Acta Agrestia Sinica, 2023, 31(4): 1064-1070.

李红玉, 王雅聪, 夏方山, 王勃, 李尹琳, 张杰敏. 燕麦种胚细胞和线粒体AsA-GSH循环对外源H₂O₂引发的响应[J]. 草地学报, 2023, 31(4): 1064-1070.

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Response of AsA-GSH Cycles to Exogenous H₂O₂ Priming in the Embryonic Cells and Mitochondria of Oat Seeds

燕麦种胚细胞和线粒体AsA-GSH循环对外源H₂O₂引发的响应

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