The Effects of Different Nitrogen Compounds Additions on Net Nitrogen Mineralization of Grassland in the Agro-pastoral Ecotone in Northern China

doi:10.11733/j.issn.1007-0435.2023.05.006

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (5): 1322-1330.DOI: 10.11733/j.issn.1007-0435.2023.05.006

The Effects of Different Nitrogen Compounds Additions on Net Nitrogen Mineralization of Grassland in the Agro-pastoral Ecotone in Northern China

ZHANG Zuo-en^1,2,3, DIAO Hua-jie^1,2,3, HAO Jie^1,2,3, YAN Xue-dong^1,2,3, GAO Yan-ping⁴, WANG Chang-hui^1,2,3, DONG Kuan-hu^1,2,3

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Shanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Taigu, Shanxi Province 030801, China;
3. Youyu Loess Plateau Grassland Ecosystem Research Station, Youyu, Shanxi Province 037200, China;
4. Agricultural and Rural Bureau of Fanshi County, Fanshi, Shanxi Province 034300, China

Received:2022-10-13 Revised:2023-04-01 Online:2023-05-15 Published:2023-05-31

不同氮化合物添加对农牧交错带草地土壤净氮矿化速率的影响

张祚恩^1,2,3, 刁华杰^1,2,3, 郝杰^1,2,3, 燕学东^1,2,3, 高艳萍⁴, 王常慧^1,2,3, 董宽虎^1,2,3

1. 山西农业大学草业学院, 山西太谷 030801;
2. 草地生态保护与乡土草种质创新山西省重点实验室, 山西太谷 030801;
3. 山西右玉黄土高原草地生态系统国家定位观测研究站, 山西右玉 037200;
4. 山西省忻州市繁峙县农业农村局, 山西繁峙 034300

通讯作者: 王常慧,E-mail:wangch@ibcas.ac.cn;董宽虎,E-mail:dongkuanhu@sxau.edu.cn
作者简介:张祚恩(1997-),男，汉族，内蒙古呼和浩特人，硕士研究生，主要从事草地生态与管理研究，E-mail：zhangzen1997@163.com
基金资助:
国家重点研发计划(2022YFF1302801);国家自然科学基金面上项目(31872406);山西省重点实验室项目(202104010910017);山西农业大学高层次引进人才项目(2021XG008)资助

Abstract

Abstract: In order to study whether it is different that the effects of different forms of nitrogen compounds on the process of nitrogen transformation in soil,the additions of different nitrogen compounds was established in the experimental platform:the Shanxi Youyu Loess Plateau Grassland Ecosystem National Positioning Observation and Research Station in 2017. This study investigated the effects of five different nitrogen compounds (ammonium nitrate (NH₄NO₃,AN),ammonium sulfate ((NH₄)₂SO₄,AS),ammonium bicarbonate (NH₄HCO₃,AC),urea (CO(NH₂)₂,UN),slow-release urea (RUN)) on the net nitrogen mineralization rate in grassland soil in the agro-pastoral ecotone. It was found out that the addition of NH₄ NO₃,(NH₄)₂SO₄,NH₄HCO₃,CO(NH₂)₂ and slow-release urea increased gene abundance of the nitrification bacteria to varying degrees. Adding ammonium nitrate and slow-release urea could more effectively increase the net nitrogen mineralization rate. Different nitrogen compounds regulate the net nitrogen mineralization rate through direct or indirect impositions on soil microorganisms.

Key words: Nitrogen compounds, Net nitrogen mineralization, Nitrification rate, Soluble organic carbon, Soluble organic nitrogen, AOA, AOB

摘要： 为研究不同氮化合物在土壤中对氮转化过程的影响是否存在差异,本研究依托山西右玉黄土高原草地生态系统国家定位观测研究站2017年建立的不同形态氮化合物添加试验平台,通过顶盖埋管法监测5种不同氮化合物(硝酸铵(NH₄NO₃,AN)、硫酸铵((NH₄)₂SO₄,AS)、碳酸氢铵(NH₄HCO₃,AC)、尿素(CO(NH₂)₂,UN)、缓释尿素(RUN))添加对农牧交错带草地土壤净氮矿化速率的影响。结果发现:添加硝酸铵、硫酸铵、碳酸氢铵、尿素与缓释尿素对硝化细菌的基因丰度均有不同程度提升;添加硝酸铵及缓释尿素会更有效地提高净氮矿化速率,不同氮化合物均是通过直接或间接影响土壤微生物实现对净氮矿化速率的调控。

关键词: 氮化合物, 净氮矿化, 硝化速率, 可溶性有机碳, 可溶性有机氮, 氨氧化细菌, 氨氧化古菌

CLC Number:

S154.1

ZHANG Zuo-en, DIAO Hua-jie, HAO Jie, YAN Xue-dong, GAO Yan-ping, WANG Chang-hui, DONG Kuan-hu. The Effects of Different Nitrogen Compounds Additions on Net Nitrogen Mineralization of Grassland in the Agro-pastoral Ecotone in Northern China[J]. Acta Agrestia Sinica, 2023, 31(5): 1322-1330.

张祚恩, 刁华杰, 郝杰, 燕学东, 高艳萍, 王常慧, 董宽虎. 不同氮化合物添加对农牧交错带草地土壤净氮矿化速率的影响[J]. 草地学报, 2023, 31(5): 1322-1330.

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The Effects of Different Nitrogen Compounds Additions on Net Nitrogen Mineralization of Grassland in the Agro-pastoral Ecotone in Northern China

不同氮化合物添加对农牧交错带草地土壤净氮矿化速率的影响

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