Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress

doi:10.11733/j.issn.1007-0435.2023.05.013

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (5): 1386-1392.DOI: 10.11733/j.issn.1007-0435.2023.05.013

Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress

HUANG Rong-yan¹, MENG Wei-shan³, ZHU Kun¹, LIU Dian-hui¹, ZHAO Zhu-ting¹, LU Cheng-wei¹, SUN Zheng-xuan¹, TANG Kuan-yu¹, ZHOU Jia-qi¹, CHENG Yu¹

1. College of Life Science, Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
2. Northeast Forestry University, Harbin, Heilongjiang Province 150040, China;
3. Animal Husbandry and Veterinary Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang Province 161006, China

Received:2022-11-23 Revised:2023-01-06 Online:2023-05-15 Published:2023-05-31

细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应

黄荣雁¹, 孟维珊³, 朱琨¹, 刘殿辉¹, 赵竹婷¹, 路程伟¹, 孙正轩¹, 唐宽宇¹, 周佳奇¹, 程瑜¹

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 东北林业大学林学院, 黑龙江哈尔滨 150040;
3. 黑龙江省农业科学院畜牧兽医分院, 黑龙江齐齐哈尔 161006

通讯作者: 黄荣雁,E-mail:hry_ok@163.com
作者简介:黄荣雁(1982-)，女，汉族，黑龙江齐齐哈尔人，副教授，博士研究生，主要从事森林培育学和林木病理学研究，E-mail:hry_ok@163.com
基金资助:
黑龙江省省属高校基本科研业务费项目(135509220)；中央财政林业科技推广示范资金项目(黑[2022]TG14号)；黑龙江省自然科学基金联合引导项目(LH2021C098)资助

Abstract

Abstract: In order to study the drought resistance of Berberis poiretii, the experiment of water control by pot culture was used to treat the seedlings of B. poiretii under drought stress,the changes of physiological characters,photosynthesis and leaf surface micromorphology of B. poiretii seedlings under mild,moderate and severe drought stress were investigated. The results showed that the relative water content (RWC) of B. poiretii leaves decreased,the content of malondialdehyde (MDA) and proline (Pro) increased with the intensification of drought stress,the contents of Superoxide dismutase (SOD)and Peroxidase (POD) reached the maximum in moderate drought,the net photosynthetic rate (P_n),stomatal conductance (G_s),transpiration rate (T_r) and intercellular CO₂ concentration (C_i) decreased with the intensification of drought stress,while the water use efficiency (WUE) increased greatly in the severe drought,the wax coverage and stomatal density of leaf surface increased with that intensification,while the stomatal size and opening degrees decreased. The results showed that B. poiretii had a certain drought-resistant ability and was suitable for cultivation in moderate arid area.

Key words: Berberis poiretii, Drought stress, Physiological characteristics, Photosynthetic characteristics, Leaf epidermis micromorphology

摘要： 为探究细叶小檗(Berberis poiretii Schneid.)抗干旱能力,本研究采用盆栽控水的方法,对细叶小檗幼苗进行干旱胁迫处理,测定轻度、中度、重度干旱胁迫下细叶小檗幼苗生理、光合及叶表微形态的变化。结果表明:随着干旱胁迫程度的加剧,细叶小檗叶片相对含水量下降,丙二醛含量、脯氨酸含量逐渐升高,超氧化物歧化酶、过氧化物酶活性在中度干旱时达到最大值;净光合速率、气孔导度、蒸腾速率和胞间CO₂浓度均随胁迫的加剧呈递减趋势,而水分利用效率在重度干旱时大幅度提高;叶表蜡质覆盖面和气孔密度随胁迫的加剧呈增大趋势,而气孔大小和开度呈减小趋势。试验表明细叶小檗具有一定的抗旱能力,适合在中度干旱地区栽培,本研究为细叶小檗的开发利用提供参考依据。

关键词: 细叶小檗, 干旱胁迫, 生理特性, 光合特性, 叶表微形态

CLC Number:

Q945.78

HUANG Rong-yan, MENG Wei-shan, ZHU Kun, LIU Dian-hui, ZHAO Zhu-ting, LU Cheng-wei, SUN Zheng-xuan, TANG Kuan-yu, ZHOU Jia-qi, CHENG Yu. Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress[J]. Acta Agrestia Sinica, 2023, 31(5): 1386-1392.

黄荣雁, 孟维珊, 朱琨, 刘殿辉, 赵竹婷, 路程伟, 孙正轩, 唐宽宇, 周佳奇, 程瑜. 细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应[J]. 草地学报, 2023, 31(5): 1386-1392.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2023.05.013

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2023/V31/I5/1386

References

[1] WAHID A,GELANI S,ASHRAF M,et al. Heat tolerance in plants:An overview[J]. Environmental and Experimental Botany,2007,61(3):199-223
[2] 何开跃,李晓储,黄利斌,等. 干旱胁迫对木兰科5树种生理生化指标的影响[J]. 植物资源与环境学报,2004,13(4):20-23
[3] GUPTA A,RICO-MEDINA A,CAÑO-DELGADO A I. The physiology of plant responses to drought[J]. Science,2020,368(6488):266-269
[4] 刘彦辰,初天舒,许亮,等. 细叶小檗的组织构造与显微特征[J]. 辽宁中医药大学学报2015,17(9):46-49
[5] 刘潇雯,吕伟旗,陈伟东,等. 6省13个产地细叶小檗中盐酸小檗碱测定与相关性分析[J]. 中成药,2021,43(3):713-716
[6] DING Y P,YE X L,ZHU J Y,et al. Structural modification of berberine alkaloid and their hypoglycemic activity[J]. Journal of Functional Foods,2014,7:229-237
[7] 王燕凌,刘君,齐曼·尤努斯,等. 植物生理学实验指导[M]. 北京:中国农业出版社,2014:14-15
[8] WEATHERLE P E. Studies in the Water Relations of the Cotton Plant. I. The Field Measurement of Water Deficits in Leaves[J]. The New Phytologist,1950,49(1):81-97
[9] BERRY J A,DOWNTON W J S. Environmental regulation of photosynthesis[J]. Photosynthesis Research,1982,2:263-343
[10] NIJS I,FERRIS R,BLUM H,et al. Stomatal regulation in a changing climate:a field study using free air temperature increase (FATI) and free air CO₂ enrichment (FACE)[J]. Plant,Cell&Environment,1997,20(8):1041-1050
[11] 朱琨,郑桂华,刘丽杰,等. 草地早熟禾叶片表皮解剖结构与抗白粉病性的研究[J]. 草地学报,2021,29(7):1430-1435
[12] 赵霖玉,李秧秧. 遮阴和干旱对白桦幼苗光诱导的气孔动力学影响[J]. 应用生态学报,2022,33(9):309-316
[13] LI Z,TAN X F,LU K,et al. The effect of CaCl₂ on calcium content,photosynthesis,and chlorophyll fluorescence of tung tree seedlings under drought conditions[J]. Photosynthetica,2017,55(3):553-560
[14] ALESSANDRA F,LUISA T,STEFANO A. Drought tolerance strategies highlighted by two Sorghum bicolor races in a drydown experiment[J]. Journal of Plant Physiology,2016(190):1-14
[15] 马少薇,刘果厚,王蕾,等. 干旱胁迫对黄柳雌雄扦插苗生长和生理特性的影响[J]. 西北植物学报,2019,39(7):1250-1258
[16] 吕玉茹,李造哲,马青枝,等. 披碱草和野大麦及其杂交新品系苗期抗旱性[J]. 江苏农业科学,2021,49(7):160-164
[17] 黄海霞,连转红,王亮,等. 裸果木渗透调节物质和抗氧化酶活性对干旱的响应[J]. 干旱区研究,2020,37(1):227-235
[18] 汪娅琴,郭小兰,李培培,等. 4个兔眼蓝莓品种对持续干旱的生理响应及其抗旱性评价[J]. 经济林研究,2021,39(3):186-196
[19] 杨耀国,牛冰洁,王永新,等. 干旱胁迫下CO₂浓度倍增对达乌里胡枝子幼苗生长及抗氧化特性的影响[J]. 草地学报,2022,30(3):661-669
[20] 冯晓敏,张永清. 水分胁迫下不同耐旱性糜子叶片保护酶活性及膜脂过氧化特性[J]. 干旱地区农业研究,2019,37(1):206-213
[21] 张雕,刘敏婕,刘卫东,等. 干旱胁迫对'菊花桃'幼苗生长及生理特性的影响[J]. 经济林研究,2021,39(1):211-219
[22] 余淑艳,周燕飞,黄薇,等. 干旱、盐及旱盐负荷胁迫对沙路草光合和生理特性的影响[J]. 草地学报,2021,29(11):2399-2406
[23] 罗孟容,梁文斌,杨艳,等. 干旱胁迫对栀子光合作用及叶绿体超微结构的影响[J]. 经济林研究,2021,39(3):165-174
[24] 边雅茹,田军仓. 灌水量对压砂地欧李光合作用及产量的影响[J]. 节水灌溉,2017(2):34-38
[25] 高冠龙,冯起,张小由,等. 植物叶片光合作用的气孔与非气孔限制研究综述[J]. 干旱区研究,2018,35(4):929-937
[26] FLEXAS J,BOTA J,GALMÉS J,et al. Keeping a positive carbon balance under adverse conditions:responses of photosynthesis and respiration to water stress[J]. Plant Physiology,2006,127:343-352
[27] CHAVES M M,FLEXAS J,PINHEIRO C. Photosynthesis under drought and salt stress:regulation mechanisms from whole plant to cell[J]. Annals Botany,2009,103:551-560
[28] LAWLOR D W,CORNIC G. Photosynthetic carbon assimilation and associated metabolism in relation to water defiits in higher plants[J]. Plant Cell & Environment,2002,25:275-294
[29] LANG Y,WANG M,XIA J B,et al. Effects of soil drought stress on photosynthetic gas exchange traits and chlorophyll fluorescence in Forsythia suspensa[J]. Journal of Forestry Research,2018,29(1):45-53
[30] LI T T,WANG R,ZHAO D Q,et al. Effects of drought stress on physiological responses and gene expression changes in herbaceous peony (Paeonia lactiflora Pall.)[J]. Plant Signaling & Behavior,2020,15(5):1746034-1746047
[31] 韩阳瑞,单炜,许大为,等. 沙地柠条光合特性与水分利用效率对干旱胁迫的响应[J]. 西南林业大学学报(自然科学版),2021,41(3):37-44
[32] 隗正阳. 干旱和盐胁迫对向日葵叶片蜡质的影响及蜡质烷烃合成基因的克隆[D]. 杨陵:西北农林科技大学,2022:5-6
[33] LIU T,OHASHI-ITO K,BERGMANN D C. Orthologs of Arabidopsis thaliana stomatal b HLH genes and regulation of stomatal development in grasses[J]. Development,2009,136:2265-2276
[34] FRANKS P J,FARQUHAR G D. The effect of exogenous abscisic acid on stomatal development,stomatal mechanics,and leaf gas exchange in Tradescantia virginiana[J]. Plant Physiology,2001,125(2):935-942
[35] 赵瑞霞,张齐宝,吴秀英. 干旱对小麦叶片下表皮细胞、气孔密度及大小的影响[J]. 内蒙古农业科技,2001(6):6-7
[36] 杨惠敏,张晓艳,王根轩. 干旱条件下两种山黧豆气孔特性及种子ODAP粗蛋白和淀粉积累的研究[J]. 兰州大学学报,2004,40(1):64-67
[37] 高秀萍,张勇强,童兆平. 梨树在自然干旱条件下叶片解剖学特征[J]. 山西农业科学,2001,29(1):62-64
[38] 高彦萍,冯莹,马志军. 水分胁迫下不同抗旱类型大豆叶片气孔特性变化研究[J]. 干旱地区农业研究,2007,25(2):77-79
[39] 于海秋,王晓磊,蒋春姬. 土壤干旱下玉米幼苗解剖结构的伤害进程[J]. 干旱地区农业研究,2008,26(5):143-147
[40] 何小三,王玉娟,徐林初,等. 干旱胁迫对不同油茶品种叶片解剖结构的影响[J]. 中南林业科技大学学报,2020,40(10):1-17

Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress

细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CEN Hui-fang, QIAN Wen-wu, ZHU Hui-sen, XIA Fang-shan, DU Li-xia, XU Tao. Effects of Drought Stress on Leaf Microstructure and Photosynthetic Characteristics of Poa pratensis [J]. Acta Agrestia Sinica, 2023, 31(5): 1368-1377.
[2]	REN Ming-hui, ZHANG Yu-peng, XU Tao, ZHU Hui-sen, CEN Hui-fang. Identification and Expression Analyses of R2R3-MYB Subfamily in Alfalfa under Drought Stress [J]. Acta Agrestia Sinica, 2023, 31(4): 972-983.
[3]	WU Xue-rui, ZHAO Qing-xia, CAI Yin-mei, ZHANG Cheng-fu, LI Guan-liang. Effects of Drought-Rewatering on Leaf Water Potential and Stomatal Opening and Closing in Broussonetia papyrifera [J]. Acta Agrestia Sinica, 2023, 31(3): 769-776.
[4]	SHI Wei-ning, SU Shi-ping, LI Yi, ZHANG Gong, XI Jie. Effects of Exogenous Proline Spraying on Stomatal Morphology of Reaumuria soongorica under Arid Environment [J]. Acta Agrestia Sinica, 2023, 31(3): 777-784.
[5]	MA Xiao-lan, ZHOU Hua-kun, ZHANG Zheng-fang, LI Zhen-zhen, XU Meng-zhen, HUANG Ao-wei, WANG Wen-ying, YIN Heng-xia. The Effects of Exogenous IAA on Seed Germination and Seedling Growth of Onobrychis viciifolia Scop. under the Drought Stress [J]. Acta Agrestia Sinica, 2023, 31(3): 796-803.
[6]	LI Hui, YUE Yue-sen, TENG Ke, ZHANG Quan-gang, TENG Wen-jun, ZHANG Hui, WEN Hai-feng, WU Ju-ying, FAN Xi-feng. Physiological Characteristics of Seed Germination of Carex rigescens at different Temperatures [J]. Acta Agrestia Sinica, 2023, 31(2): 426-432.
[7]	LI Chun-yan, ZHONG Hua, DU Li-xia, HOU Xiang-yang. The Cloning BiMYB52 Gene from Bothriochloa ischaemum and Analysis of Drought Resistance Expression of Transgenic Arabidopsis thaliana of that clone [J]. Acta Agrestia Sinica, 2023, 31(1): 29-39.
[8]	LIU Shu-lan, LI Jin, MA Yong-hui, WU Li-mei, LI Ying, ZHU Li-chun. Effects of Strigolactones on Seed Germination and Seedling Physiological Changes of Lycium ruthenicum under Drought Stress [J]. Acta Agrestia Sinica, 2023, 31(1): 130-139.
[9]	YANG Hang, ZHOA Ya-jiao, LIU Xiao-jing. Effects on Photosynthesis Characteristics and Yield Regulations in the Alfalfa/Oat Intercropping [J]. Acta Agrestia Sinica, 2023, 31(1): 187-195.
[10]	LYU Yan-min, HUA Mei, ZHANG Xiao-ming, MU Ying-tong, LI Xiao-jie, HAO Ning, AO Yun, WANG Jun-jie. Effects of Storage Years on Seed Germination,Seedling Growth and Physiological Characteristics of White Clover [J]. Acta Agrestia Sinica, 2023, 31(1): 287-293.
[11]	NI Wang, LIANG Dan-ni, SHI Xing-wei, WANG Bin, WANG Teng-fei, LI Man-you, FENG Qin, LAN Jian. Effects of Irrigation Frequency and Fertilizer Application Rate on Production Performance and Photosynthetic Characteristics of Sweet Sorghum [J]. Acta Agrestia Sinica, 2023, 31(1): 294-301.
[12]	ZHANG Yu-juan, GUO Rui, ZHANG Zhao-heng, ZHANG Yan, MA Hui-ling, DONG Wen-ke. Effect of Clod Plasma Treatment on Salt Resistance of Poa pratensis Seedlings [J]. Acta Agrestia Sinica, 2022, 30(9): 2365-2374.
[13]	QI Shuai, QIN Wei-na, JIAO Ting, SHI Shang-li, GAO Yong-quan, WANG Hu-ning, LI Shu-yan. Evaluation on Physiological Characteristics and Water Use Efficiency for Different Silage Maize Varieties in Gansu Rain-Fed Area [J]. Acta Agrestia Sinica, 2022, 30(9): 2407-2414.
[14]	HE Ji-tong, MA Xiang, JU Ze-liang, LIU Kai-qiang, ZHAO Ji-xiu, MA Xiao-long, JIA Zhi-feng. Effects of Intercropping between Oat and Broad Bean on Crop Growth and Yield in Alpine Region [J]. Acta Agrestia Sinica, 2022, 30(9): 2514-2521.
[15]	CHEN Xin, JIAO Ting, MU Ren, WANG Hu-ning, ZHANG Xia, MA Shu-min, GAO Yong-quan, LI Shu-yan, QI Shuai. Effects of Nitrogen Addition on Growth and Photosynthetic Characteristics of Elymus nutans in Alpine Artificial Grassland [J]. Acta Agrestia Sinica, 2022, 30(8): 1964-1971.