Metabolomics-based Study of Root Metabolites Associated with Nodule in Trifolium ambiguum Bieb. 'Mengnong No.1'

doi:10.11733/j.issn.1007-0435.2023.09.014

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (9): 2703-2711.DOI: 10.11733/j.issn.1007-0435.2023.09.014

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Metabolomics-based Study of Root Metabolites Associated with Nodule in Trifolium ambiguum Bieb. 'Mengnong No.1'

LIU Jia-wei¹, WANG Ming-jiu¹, CAO Ke-fan¹, HE Li-jun², HAO Xin-yan¹, ZHANG Hui-min¹, HAO Lu¹

1. Key Laboratory of Grassland Resources of Ministry of Education/College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010011, China;
2. Agricultural College of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China

Received:2023-03-13 Revised:2023-04-12 Online:2023-09-15 Published:2023-10-07

基于代谢组学的‘蒙农三叶草1号’与结瘤相关的根系代谢物研究

刘嘉伟¹, 王明玖¹, 曹克璠¹, 何丽君², 郝新艳¹, 张慧敏¹, 郝璐¹

1. 内蒙古农业大学草地资源教育部重点实验室/草原与资源环境学院, 内蒙古呼和浩特 010011;
2. 内蒙古农业大学农学院, 内蒙古呼和浩特 010019

通讯作者: 王明玖,E-mail:wangmj_0540@163.com
作者简介:刘嘉伟(1992-),男,汉族,内蒙古呼伦贝尔人,博士研究生,主要从事牧草遗传育种研究,E-mail:nmgndliu_1105@163.com
基金资助:
国家自然科学基金“‘蒙农三叶草1号’结瘤固氮机理及根瘤菌多样性研究”(31960353)；国家自然科学基金“‘蒙农三叶草1号’花期延长及其调控机理研究”(32160334)资助

Abstract

Abstract: To investigate the changes of root metabolites in Caucasian clover after intercropping with rhizobia,this study used Trifolium ambiguum Bieb. ‘Mengnong No. 1’ The results showed that the root metabolites of T. ambiguum Bieb. ‘Mengnong No. 1’ were analyzed after inoculation with rhizobia and the metabolomics technique was applied. The results showed that after inoculation with rhizobia,the root system of T.ambiguum Bieb. ‘Mengnong No. 1’ displayed nodulation phenomenon,and the content of 249 compounds changed significantly,mainly in the process of "biosynthesis of other secondary metabolites". A total of 75 compounds were enriched in 47 pathways,mainly in the processes of "flavone and flavonol biosynthesis" and "flavonoid biosynthesis". Among them,oxoglutaric acid,kaempferol,quercetin,phloridzin,myricetin and glycitin promote the nodulation process of this clover,and arginine promotes the growth of root nodules. This study may provide a theoretical reference for improving the nodulation efficiency of Caucasian clover for late time.

Key words: Trifolium ambiguum Bieb. ‘Mengnong No.1’, Caucasian Clover, Nodular nitrogen fixation, Metabolomics, Root metabolites

摘要： 为探究高加索三叶草与根瘤菌互作后根系代谢物的变化,本研究以'蒙农三叶草1号'高加索三叶草(Trifolium ambiguum Bieb. 'Mengnong No.1')为试验材料,通过设置接种与不接种根瘤菌处理,观察其结瘤效果,并运用代谢组学技术分析接种根瘤菌后根系代谢产物的变化。结果表明,在接种根瘤菌后,'蒙农三叶草1号'根系出现结瘤现象,共有249个化合物含量发生显著变化,主要注释在"次生代谢产物生物合成"过程中;共有75个化合物富集在47个通路中,主要富集在"黄酮和黄酮醇的生物合成"和"类黄酮生物合成"过程中。其中有16个差异代谢物在结瘤过程中起重要作用,酮戊二酸、山奈酚、槲皮苷、根皮苷、杨梅素和黄豆黄甙促进了该三叶草结瘤过程,精氨酸促进根瘤的生长。本研究可为后期提高高加索三叶草结瘤效率提供理论参考。

关键词: 蒙农三叶草1号', 高加索三叶草, 结瘤固氮, 代谢组学, 根系代谢产物

CLC Number:

S541+2

LIU Jia-wei, WANG Ming-jiu, CAO Ke-fan, HE Li-jun, HAO Xin-yan, ZHANG Hui-min, HAO Lu. Metabolomics-based Study of Root Metabolites Associated with Nodule in Trifolium ambiguum Bieb. 'Mengnong No.1'[J]. Acta Agrestia Sinica, 2023, 31(9): 2703-2711.

刘嘉伟, 王明玖, 曹克璠, 何丽君, 郝新艳, 张慧敏, 郝璐. 基于代谢组学的‘蒙农三叶草1号’与结瘤相关的根系代谢物研究[J]. 草地学报, 2023, 31(9): 2703-2711.

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Metabolomics-based Study of Root Metabolites Associated with Nodule in Trifolium ambiguum Bieb. 'Mengnong No.1'

基于代谢组学的‘蒙农三叶草1号’与结瘤相关的根系代谢物研究

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