Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress

doi:10.11733/j.issn.1007-0435.2023.11.011

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (11): 3322-3333.DOI: 10.11733/j.issn.1007-0435.2023.11.011

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Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress

LI Shuo¹, LI Pei-ying^1,2,3, SUN Zong-jiu^1,2,3, LI Wen¹

1. College of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
2. Key Laboratory of Grassland Resources and Ecology of Xinjiang, Urumqi, Xinjiang 830052, China;
3. Key Laboratory of grassland resources and ecology ofwestern arid region, Urumqi, Xinjiang 830052, China

Received:2023-04-27 Revised:2023-05-31 Online:2023-11-15 Published:2023-12-01

敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析

李硕¹, 李培英^1,2,3, 孙宗玖^1,2,3, 李雯¹

1. 新疆农业大学草业与环境科学学院, 新疆乌鲁木齐 830052;
2. 新疆草地资源与生态自治区重点实验室, 新疆乌鲁木齐 830052;
3. 西部干旱区草地资源与生态教育部重点实验室, 新疆乌鲁木齐 830052

通讯作者: 李培英,E-mail:nmlpy_1234@sina.com
作者简介:李硕(1998-),女,汉族,内蒙古巴彦淖尔人,硕士研究生,主要从事草种资源评价与利用研究,E-mail:2438354986@qq.com
基金资助:
国家自然科学基金项目(31960362)资助

Abstract

Abstract: Bermudagrass [Cynodon dactylon (Linn.) Pers.] is an excellent drought-resistant turfgrass,and the drought-resistant molecular mechanism needs to be further clarified. In this study,the drought-resistant (C138) and drought-sensitive (C32) bermudagrass genotypes were treated under normal irrigation (soil relative water content of 80%~90% of the field water capacity),moderate drought (50%~60%) and severe (20%~30%) drought for 10 days,then the leaves were sampled for transcriptome sequencing by Illumina High-throughput sequencing platform. Compared with normal irrigation,1 086 up-regulated genes expressed for C32 under drought stress,along with 525 up-regulated for C138. The number of drought response genes in C32 was higher than C138,which indicated that C32 mainly would take a respond to drought through positive gene regulation. Differential gene GO enrichment analysis showed that C138 had more photosynthesis-related gene responded under drought stress,while C32 genes enriched on cell division. KEGG enrichment analysis showed that C138 genes mainly were enriched in chlorophyll metabolism and diterpenoid biosynthesis under moderate stress,while C32 genes mainly in nitrogen metabolism and flavonoid biosynthesis. Under severe stress,C138 was mainly enriched in flavonoid biosynthesis,while C32 mainly in nitrogen metabolism and benzoxazine biosynthesis. Drought resistance of Bermudagrass under moderate stress may be related to photosynthesis,the secondary metabolism and osmotic protection under severe stress. Nitrogen metabolism in secondary metabolism,biosynthesis of benzoxazines and glutamate metabolism in osmotic protection may be important enrichment pathways for drought resistance of bermudagrass.

Key words: Drought stress, Bermudagrass, Leaf, Transcriptome sequencing

摘要： 狗牙根[Cynodon dactylon (Linn.) Pers]是一种优质抗旱草坪草，但其抗旱分子机制有待进一步明确。本研究以抗旱(C138)和敏旱(C32)2个狗牙根基因型为材料，对其正常灌溉(土壤相对含水量为田间持水量的80%~90%)、中度干旱(50%~60%)及重度(20%~30%)干旱处理10 d后的叶片进行取样，利用Illumina高通量测序平台进行转录组测序。与正常灌溉相比，干旱胁迫下C32有1 086个上调基因；C138有525个上调基因；C32整体响应基因数量更多，说明C32主要通过基因的正调控来响应干旱。差异基因GO富集分析显示，C138受到干旱胁迫时有更多的光合作用基因响应，C32主要富集在细胞分裂上。KEGG富集分析显示，中度胁迫下C138主要富集在叶绿素代谢和二萜类生物合成，C32主要富集在氮代谢和类黄酮生物合成；重度胁迫下C138主要富集在类黄酮生物合成，C32主要富集在氮代谢、苯并恶嗪类生物合成。中度胁迫下狗牙根抗旱可能与光合作用有关，重度胁迫下主要与次生代谢和渗透保护有关。次生代谢中的氮代谢、苯并恶嗪类生物合成和渗透保护中的谷氨酸代谢可能是狗牙根抗旱的重要富集途径。

关键词: 干旱胁迫, 狗牙根, 叶片, 转录组分析

CLC Number:

S688.4

LI Shuo, LI Pei-ying, SUN Zong-jiu, LI Wen. Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress[J]. Acta Agrestia Sinica, 2023, 31(11): 3322-3333.

李硕, 李培英, 孙宗玖, 李雯. 敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析[J]. 草地学报, 2023, 31(11): 3322-3333.

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Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress

敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析

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