Effects of Lactic Acid Bacteria and Mixed Acids on Fermentation Quality,Chlorophyll and Chlorophyll Degrading Enzymes in Alfalfa Silage

doi:10.11733/j.issn.1007-0435.2024.05.031

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (5): 1610-1618.DOI: 10.11733/j.issn.1007-0435.2024.05.031

Effects of Lactic Acid Bacteria and Mixed Acids on Fermentation Quality,Chlorophyll and Chlorophyll Degrading Enzymes in Alfalfa Silage

KONG De-hui^1,2, JIN Si-yu³, CAO Yang^1,2,4

1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Heilongjiang Provincial Key Laboratory of Efficient Utilization of Feed Resources and Nutrition Manipulation in Cold Region, Daqing, Heilongjiang Province 163319, China;
2. Engineering Research Center of Processing and Utilization of Grain By-products, Ministry of Education, Daqing, Heilongjiang Province 163319, China;
3. Muyuan Foods Co., Ltd., Nanyang, Henan Province 47300, China;
4. Key Laboratory of Low-carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P. R. China, Daqing, Heilongjiang Province 163319, China

Received:2023-12-04 Revised:2024-03-19 Published:2024-06-01

乳酸菌和混合酸对苜蓿青贮品质、叶绿素及其降解酶的影响

孔德慧^1,2, 靳思玉³, 曹阳^1,2,4

1. 黑龙江八一农垦大学动物科技学院, 黑龙江省寒区饲料资源高效利用与营养调控重点实验室, 黑龙江大庆 163319;
2. 粮食副产物加工与利用教育部工程研究中心, 黑龙江大庆 163319;
3. 牧原食品股份有限公司, 河南南阳 473000;
4. 农业农村部东北平原农业绿色低碳重点实验室, 黑龙江大庆 163319

通讯作者: 曹阳，E-mail:hbdkcaoyang@163.com
作者简介:孔德慧(1999-)，女，汉族，黑龙江大庆人，硕士研究生，主要从事反刍动物营养研究，E-mail:1955857122@qq.com
基金资助:
黑龙江省重点研发计划指导类项目(GZ20230029)；国家自然科学基金项目(No.32171676)；黑龙江省“百千万”工程科技重大专项(No.2021ZX12B03)资助

Abstract

Abstract: In order to study the changes of chlorophyll and chlorophyll degrading enzyme contents in alfalfa (Medicago sativa L.) silage,we used a two-factor test design (Additive×3,Days×13),with two additive treatments of adding lactic acid bacteria (LAB,Lactobacillus plantarum,10⁵cfu·g^-1 of fresh matter basis) or mixed acid (MA) (4% fresh matter basis),in comparison with the control without additive materials (CK). The results showed that the silage treated with LAB and MA addition exhibited a lower pH and higher lactic acid content than those of the CK. The contents of chlorophyll a and chlorophyll b in CK were lower than those in the treatment with LAB,but higher than those in the treatment with MA. The addition of LAB inhibited the activity of chlorophyll degrading enzymes and slowed the degradation of chlorophyll,while MA showed the opposite pattern. In summary,LAB addition improved the fermentation quality of alfalfa silage,and obtained the highest chlorophyll content and the lowest activity of chlorophyll degrading enzyme in alfalfa silage. This study laid a practical and theoretical foundation for developing feed additives and increasing chlorophyll content in alfalfa silage.

Key words: Fermentation quality, Alfalfa silage, Microbial composition, Chlorophyll, Chlorophyll degrading enzymes

摘要： 为了探究紫花苜蓿(Medicago sativa L.)青贮过程中叶绿素及其降解酶含量的变化规律，试验采用双因素试验设计(添加剂×3，天数×13)，处理包括对照组(Control)、添加乳酸菌(Lactic acid bacteria，LAB)(植物乳杆菌，10⁵cfu·g^-1新鲜物质基础)组和添加混合酸(Mixed acid，MA)(添加量4%)组。结果表明：与对照青贮相比，LAB和MA处理的青贮pH值较低，乳酸含量较高。对照组中叶绿素a和叶绿素b含量低于LAB组，高于MA组。在苜蓿青贮过程中，LAB抑制了叶绿素降解酶的活性，抑制了叶绿素的降解，MA则相反。综上：添加LAB可以改善紫花苜蓿青贮发酵品质，且LAB组的紫花苜蓿青贮中叶绿素含量最高，叶绿素降解酶含量最低。本研究为调制生产富含功能性成分的苜蓿青贮饲料提供了理论基础。

关键词: 发酵品质, 苜蓿青贮, 微生物组成, 叶绿素, 叶绿素降解酶

CLC Number:

S816.5+3

KONG De-hui, JIN Si-yu, CAO Yang. Effects of Lactic Acid Bacteria and Mixed Acids on Fermentation Quality,Chlorophyll and Chlorophyll Degrading Enzymes in Alfalfa Silage[J]. Acta Agrestia Sinica, 2024, 32(5): 1610-1618.

孔德慧, 靳思玉, 曹阳. 乳酸菌和混合酸对苜蓿青贮品质、叶绿素及其降解酶的影响[J]. 草地学报, 2024, 32(5): 1610-1618.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2024.05.031

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2024/V32/I5/1610

References

[1] BOUIZGAREN A,FARISSI M,GHOULAM C,et al. Assessment of summer drought tolerance variability in Mediterranean alfalfa (Medicago sativa L.) cultivars under Moroccan fields conditions[J]. Archives of Agronomy and Soil Science,2013,59(1):14
[2] LI X,SU D,YUAN Q. Ridge-furrow planting of alfalfa (Medicago sativa L.) for improved rainwater harvest in rainfed semiarid areas in Northwest China[J]. Soil and Tillage Research,2007,93(1):117-125
[3] CAJARVILLE C,GONZALEZ J,REPETTO J L,et al. Nutritive value of green forage and crop by-products of Cynara cardunculus[J]. Annales De Zootechnie,1999,48(5):353-365
[4] ZHANG Y,LIU C,LIU S,et al. Structural stability and properties of three isoforms of the major light-harvesting chlorophyll a/b complexes of photosystem II[J]. Biochimicaet Biophysica Acta-bioenergetics,2008,1777(6):479-487
[5] SCHNFELD P,REISER G. Brain lipotoxic city of phytanic acid and very long-chain fatty acids. harmful cellular/mitochondrial activities in Refsum disease and x-linked adrenoleukodystrophy[J]. Aging and Disease,2016,7(2):136-149
[6] HASPERU J H,GMEZ-LOBATO M E,CHAVES A R,et al. Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli[J]. Postharvest Biology and Technology,2013,82(4):22-27
[7] CHEW B P,WONG M,WONG T S. Effects of lutein from marigold extract on immunity and growth of mammary tumors in mice[J]. Anticancer Research,1996,16:3689-3694
[8] BROWN P J,MEI G,GLBBERD F B,et al. Diet and refsums disease-the determination of phytanic acid and phytol in certain foods and the application of this knowledge to the choice of suitable convenience foods for patients with refsums disease[J]. Journal of Human Nutrition and Dietetics,1993,6:295-305
[9] ABIDI F,GIRAULT T,DOUILLET O,et al. Blue light effects on rose photosynthesis and photomorphogenesis[J]. Plant Biology,2013,15(1):67-74
[10] GIRON D,DICKE M. Cytokinins as key regulators in plant-microbe-insect interactions:connecting plant growth and defence[J]. Functional Ecology,2013,27(3):599-609
[11] DAVID,CHAGN,KUI,LIN-WANG,RICHARD V,ESPLEY,et al. An ancient duplication of apple MYB transcription factors is responsible for novel red fruit-flesh phenotypes[J]. Plant Physiology,2013,161(1):225
[12] GARAY L A,BOUNDYMILLS K L,GERMAN J B. Accumulation of high-value lipids in single-cell microorganisms:a mechanistic approach and future perspectives[J]. Journal of Agricultural and Food Chemistry,2014,62(13):2709-2727
[13] BRUHN J C,OLIVER J C. Effect of storage on tocopherol and carotene concentrations in alfalfa hay[J]. Journal of Dairy Science,1978,61:980-982
[14] LINDQVIST H,NADEAU E,JENSEN S K. Alpha-tocopherol and β-carotene in legume-grass mixtures as influenced by wilting,ensiling and type of silage additive[J]. Grass and Forage Science,2012,67:119-128
[15] CAO Y,CAI Y,TAKAHASHI T,et al. Effect of lactic acid bacteria inoculant and beet pulp addition on fermentation characteristics and in vitro ruminal digestion of vegetable residue silage[J]. Journal of Dairy Science,2011,94(8):3902-3912
[16] HIROSHI SUTOU. Method of preparation and utilization of silage[M]. Japan:Youkendou Publishers Inc,1968:34-36
[17] 冯宗慈,高民. 通过比色测定瘤胃液氨氮含量方法的改进[J]. 畜牧与饲料科学,2010,31(Z1):37
[18] AOAC. Official methods of analysis[M]. Arlington,VA:Association of Official Analytical Chemists,1990,15(8):33-35
[19] VAN SOEST P J,ROBERTSON J B,LEWIS B A. Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science,1991,74(10):3583-3597
[20] 闫震,聂继云,程杨,等. 水果、蔬菜及其制品中叶绿素含量的测定[J]. 中国果树,2018(2):59-62,7
[21] FUNAMOTO Y,YAMAUCHI N,SHIGENAGA T,et al. Effects of heat treatment on chlorophyll degrading enzymes in stored broccoli (Brassicaoleracea L.)[J]. Postharvest Biology and Technology,2002,24(2):163-170
[22] KAEWSUKSAENG S,KANLAYANARAT S,FUNAMOTO Y,et al. Effect of heat treatment on Mg-dechelation activity in relation to chlorophyll degradation during storage of broccoli florets[J]. Acta Horticulturae,2007,746(746):375-380
[23] 陈丹丹,黄佩珊,张超,等. 植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响[J]. 草地学报,2023,31(7):2232-2240
[24] ALLI I,FAIRBAIRN R,NOROOZI E,et al. The effects of molasses on the fermentation of chopped whole-plant leucaena[J]. Journal of The Science of Food and Agriculture,2010,35(3):285-289
[25] OKINE A,YIMAMU A,HANADA M,et al. Ensiling characteristics of daikon (Raphanussativus) by-product and its potential as an animal feed resource[J]. Animal Feed Science and Technology,2007,136(3-4):1-264
[26] DAVIES D R,MERRY R J,WILLIAMS A P,et al. Proteolysis during ensilage of forages varying in soluble sugar content[J]. Journal of Dairy Science,1998,81(2):444-453
[27] WOOLFORD M K. The microbiology of silage[M]. New York:The Silage Fermentation,1984:23-70
[28] FANG J,CAO Y,MATSUZAKI M,et al. Effects of apple pomace proportion levels on the fermentation quality of total mixed ration silage and its digestibility,preference and ruminal fermentation in beef cows[J]. Animal Science Journal,2015,87(2):217-223
[29] 张志登,蒋再慧,韩雅慧,等.乳酸菌及酸处理对秸秆生物发酵饲料的化学成分及In vitro甲烷生成的影响[J]. 黑龙江八一农垦大学学报,2016,28(6):8-15
[30] 李青璞,白建海,姚拓,等. 微生物菌剂与氮肥配施对紫花苜蓿生长及土壤性质的影响[J]. 草地学报,2024,32(1):314-321
[31] SHELLITO S M,WARD M A,LARDY G P,et al. Effects of concentrated separator by-product (desugared molasses) on intake,ruminal fermentation,digestion,and microbial efficiency in beef steers fed grass hay[J]. Journal of Animal Science,2006,84(6):1535
[32] TAKAHASHI T,HORIGUCHI K,YOSHIDA N,et al. Effect of cellulase and urea addition on in situ degradability and preference for pruned persimmon branch chips in cattle[J]. Grassland Science,2011,57(4):179-183
[33] 陈龙宾,马毅,韩静,等. 不同配比的木醋液与甲酸对苜蓿青贮品质的影响[J]. 中国饲料,2018(3):50-53
[34] CAI Y,BENNO Y,OGAWA M AND KUMAI S. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage[J]. Journal of Dairy Science,1999,82(3):520-526
[35] CAO Y,TAKAHASHI T,HORIGUCHI K I,et al. Effect of adding lactic acid bacteria and molasses on fermentation quality and in vitro ruminal digestion of total mixed ration silage prepared with whole crop rice[J]. Grassland Science,2010,56(1):19-25
[36] CAO Y,CAI Y,HIRAKUBO T,et al. Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons[J]. Animal Science Journal,2011,82(2):259-266
[37] DUNIERE L,JIN L,SMILEY B,et al. Impact of adding strains on fermentation,aerobic stability,nutritive value,and select lactobacilli populations in corn silage[J]. Journal of Dairy Science,2015,93(5):2322
[38] 蒋再慧,侯建军,邱胜桥,等. 乳酸菌制剂对苜蓿青贮发酵品质及营养价值的影响[J]. 黑龙江畜牧兽医,2017(11):147-150
[39] JACOB-WILK D. Chlorophyll breakdown by chlorophyllase:Isolation and functional expression of the Chlase 1 gene from ethylene-treated citrus fruit and its regulation during development[J]. Plant Journal,1999,20(6):653-661
[40] JOHN P MCCOOK,PETER L DOROGI,et al. In vitro inhibition of hyaluronidase by sodium copper chlorophyllin complex and chlorophyllin analogs[J]. Clinical Cosmetic & Investigational Dermatology,2015(8):443-448
[41] NAM W,KIM I,LIM M H,et al. Isolation of an oxomanganese (V) porphyrin intermediate in the reaction of a manganese (III) porphyrin complex and H₂O₂ in aqueous solution[J]. Chemistry,2015,8(9):2067-2071
[42] BVCHERT A M,CIVELLO P M,MARTNEZ G A. Chlorophyllase versus pheophytinase as candidates for chlorophyll dephytilation during senescence of broccoli[J]. Journal of Plant Physiology,2011,168(4):37-43
[43] KOCA N,KARADENIZ F,BURDURLU H S. Effect of pH on chlorophyll degradation and colour loss in blanched green peas[J]. Food Chemistry,2006,100:609-615
[44] SCHELBERT S,AUBRY S,BURLA B,et al. Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in arabidopsis[J]. Plant Cell,2009,21:767-785
[45] PRUZINSKA A,TANNER G,ANDERS I,et al. Chlorophyll breakdown:pheophorbide a oxygenase is a rieske-type iron-sulfur protein,encoded by the accelerated cell death 1 gene[J]. Proceedings of the National Academy of Sciences,2003,100(25):15259-15264
[46] CHUNG D W,PRUZINSK A,HRTENSTEINER S,et al. The role of pheophorbide a oxygenase expression and activity in the canola green seed problem[J]. Plant Physiology,2006,142(1):88-97

Effects of Lactic Acid Bacteria and Mixed Acids on Fermentation Quality,Chlorophyll and Chlorophyll Degrading Enzymes in Alfalfa Silage

乳酸菌和混合酸对苜蓿青贮品质、叶绿素及其降解酶的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HEI Meng-qin, CAO Zheng-yu, WANG Zhi-yuan, DAI Chun-hui, CHEN Cheng, ZHANG Wen-ju. Effects of Adding Compound Lactobacillus and Molasses on the Quality of Alfalfa Silage with Different Moisture Contents [J]. Acta Agrestia Sinica, 2024, 32(5): 1601-1609.
[2]	SUN Rong, LI Mao, CHEN Gui-wu, ZI Xue-juan, WANG Kai. Effects of Callicarpa Nudiflora Addition on Silage Fermentation Quality and in vitro Gas Production in King Grass [J]. Acta Agrestia Sinica, 2024, 32(4): 1267-1275.
[3]	HUANG Li-ying, CUI Wei-dong, TIAN Jing, ZHANG Jian-guo. Effects of Soybean Straw and Rice Straw on Mixed Silage of Sweet Corn Straw and Wet Brewers Grains [J]. Acta Agrestia Sinica, 2024, 32(3): 945-951.
[4]	JIN Li-na, JIANG Su-su, JING Shu-yan, HE Jian-wen, ZHANG Hui-jun. Effects of Adding Astragalus Bunge Residue on the Sensory Evaluation, Fermentation Quality, and Nutritive Value of Whole-crop Corn Silage [J]. Acta Agrestia Sinica, 2024, 32(2): 630-636.
[5]	SHI Jia-qi, LIU Zhong-kuan, WANG Dong-kui, ZHANG Li-feng, YU He-xing, ZHI Jian-fei, LI Jie-xin, LI Min, LIU Zhen-yu, XIE Nan, LIU Hui-zhi. Analysis of Photosynthetic Characteristics and Yield Traits of Fourteen Alfalfa Varieties in Coastal Saline-alkali Soil in Hebei Province [J]. Acta Agrestia Sinica, 2023, 31(7): 2107-2115.
[6]	MEN Lu, HE Yi-cheng, LI Ting-ting, LI Peng-yu, HU Jian, ZHOU Qing-ping. Nonlinear Responses and Mechanisms of Leaf Chlorophyll in Alpine Plants to Nitrogen Addition Gradient [J]. Acta Agrestia Sinica, 2023, 31(6): 1622-1631.
[7]	GU Xue-dan, ZHANG Zheng-zhong, LYU Dong, ZHAO Hu, WANG Li, CHE Bo, CAO Rong, YAN Ke-lin, ZHANG Hong-bin. Effects of Photoperiod and Light Quality on the Growth and Chlorophyll Fluorescence of Reaumuria Soongorica Seedlings [J]. Acta Agrestia Sinica, 2023, 31(6): 1720-1727.
[8]	YU Si-min, LUO Yong-zhong, KANG Fang-ming, QIU Ying-de, LI Zhuan-tao. Effects of Drought Stress on Growth and Chlorophyll Fluorescence Characteristics of Medicago sativa L. [J]. Acta Agrestia Sinica, 2023, 31(6): 1762-1771.
[9]	LI Xiao, CHEN Yong-cheng, HUANG Rong-zheng, QI Ya-yin, ZHANG Fan-fan, MA Chun-hui. Effects of Compound Starter on Silage Quality and Rumen Degradation Rate of Sunflower Byproduct [J]. Acta Agrestia Sinica, 2023, 31(5): 1564-1570.
[10]	CHAI Ji-kuan, ZHAO Gui-qin, JU Ze-liang. Effects of Adding Different Lactic Acid Bacteria on Oat Silage Fermentation at Low Temperatures [J]. Acta Agrestia Sinica, 2023, 31(3): 923-928.
[11]	ZHAN Jia-qi, CHEN Dan-dan, GUO Tian-xin, GUO Xiang, WU Shuo, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. Effects of Different Additives on Silage Quality of Chinese Medicine Residue [J]. Acta Agrestia Sinica, 2023, 31(12): 3851-3857.
[12]	GAO Long, ZHANG Juan, GE Chen-yan, LIU Kuan, TANG Jia-qi, SHI Shen-bing, YANG Fu-lin, ZHOU Jing. Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum [J]. Acta Agrestia Sinica, 2023, 31(12): 3867-3875.
[13]	TIAN Pei-xin, SONG Rui, LI Chao-ran, NI Hao-ran, WANG Run, LIU Ping, ZHANG Han, MAO Pei-sheng, JIA Shan-gang. Chloroplast Photosynthesis during Seed Germination of Smooth Bromegrass and Oat [J]. Acta Agrestia Sinica, 2023, 31(10): 3058-3066.
[14]	BAO Wen-long, ZHENG Cheng-zhong, ZHANG Teng-wei, YUN Ying, HAN Yun-peng, LIU Zhi-ping, BA Tu, XU Guang-xiang, WANG Feng-wu, SUN Juan-juan. Effects of Nitrogen Application rate on Production Performance and Silage Quality of Whole Plant Barley [J]. Acta Agrestia Sinica, 2023, 31(10): 3167-3173.
[15]	LI Hai-ping, GUAN Hao, JIA Zhi-feng, LIU Wen-hui, CHEN Shi-yong, XU Mei-ling, CHEN Sheng-cui, YAN Dong-hai, JIANG Yong-mei, GAN Li, ZHANG Jia-min, ZHOU Qing-ping. Effects of Wheat Bran and Lactic Acid Bacteria on Baled Oat Silage Quality and Aerobic Stability in the Alpine Region of Northwest Sichuan [J]. Acta Agrestia Sinica, 2023, 31(1): 302-310.