The Effects of Nitrogen Addition on Arbuscular Mycorrhizal Fungal Communities in Alpine Meadow of The Qinghai-Tibetan Plateau

doi:10.11733/j.issn.1007-0435.2024.07.006

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (7): 2054-2061.DOI: 10.11733/j.issn.1007-0435.2024.07.006

The Effects of Nitrogen Addition on Arbuscular Mycorrhizal Fungal Communities in Alpine Meadow of The Qinghai-Tibetan Plateau

GUO Xian-bao¹, HAN Bing¹, LU Xin-min², HE Yi-cheng¹, YU Lu¹, ZHANG Wan-tong¹, DUAN Li-hui¹, GENG Yi-yi¹, SHAO Xin-qing¹, YU Dian¹, HAN Jia-yi¹

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Tianshui Fruit Research Institute, Tianshui, Gansu Province 741000, China

Received:2024-03-15 Revised:2024-04-23 Published:2024-08-03

氮添加对青藏高原高寒草甸丛枝菌根真菌群落的影响

国显宝¹, 韩冰¹, 逯新民², 何奕成¹, 于露¹, 张万通¹, 段丽辉¹, 耿依仪¹, 邵新庆¹, 俞点¹, 韩伽怡¹

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 天水市果树研究所, 甘肃天水 741000

通讯作者: 邵新庆，E-mail:shaoxinqing@163.com
作者简介:国显宝(2003-)，男，汉族，辽宁朝阳人，本科生，主要从事草地生态学研究，E-mail：15114204093@163.com
基金资助:
国家自然科学基金面上项目(32171685)资助

Abstract

Abstract: Arbuscular mycorrhizal fungi (AMF) can form symbiotic relationship with the roots of most higher plants,and exchange important materials and energy. Nitrogen addition has a series of effects on AMF. Taking alpine meadow as the research object,change characteristics of AMF community and soil driving factors under four different nitrogen addition levels were studied. The results showed that:(1) Under the treatments of N5 and N10,soil pH decreased significantly,while under the treatment of N10,soil nitrate content increased significantly. (2) Under N5 and N15 treatments,the relative abundance of Diversisporaceae community decreased significantly;(3) Under N5 and N10 treatments,chao1 index decreased significantly;under N10 and N15 treatments,β diversity changed significantly;(4) The contents of soil ammonium nitrogen,nitrate nitrogen and organic carbon were the key factors affecting the community structure of AMF. Therefore,nitrogen addition affected the AMF community of alpine meadow soil in Qinghai-Tibet Plateau,and the community structure and abundance were significantly related to soil characteristics. The research results are of great significance for predicting the future changes of AMF diversity in alpine meadow of Qinghai-Tibet Plateau under the background of global atmospheric nitrogen deposition.

Key words: Nitrogen addition, Arbuscular mycorrhizal fungi, Soil chemical composition, Community composition, Diversity

摘要： 丛枝菌根真菌(Arbuscular mycorrhizal fungi，AMF)能与大部分高等植物的根系形成共生关系，进行重要的物质和能量交换，而氮添加会对AMF产生一系列影响。本试验以高寒草甸为研究对象，探究4个不同氮添加水平下土壤AMF群落的变化特征及土壤驱动因子。研究表明：(1)N5和N10处理下，土壤pH值显著降低，N10处理下，土壤硝态氮含量显著上升。(2)在N5和N15处理下，多样孢囊霉科(Diversisporaceae)群落相对丰度显著降低，(3)在N5和N10处理下，Chao1指数显著降低，在N10和N15处理下，β多样性有显著变化。(4)土壤铵态氮，硝态氮和有机碳含量是影响AMF群落结构的关键因子。因此,氮添加会影响青藏高原高寒草甸土壤AMF群落,且群落结构和丰度与土壤特性显著相关，研究结果对于预测全球大气氮沉降背景下青藏高原高寒草甸土壤AMF多样性的未来变化具有重要意义。

关键词: 氮添加, AMF, 土壤化学成分, 群落组成, 多样性

CLC Number:

S153.6

GUO Xian-bao, HAN Bing, LU Xin-min, HE Yi-cheng, YU Lu, ZHANG Wan-tong, DUAN Li-hui, GENG Yi-yi, SHAO Xin-qing, YU Dian, HAN Jia-yi. The Effects of Nitrogen Addition on Arbuscular Mycorrhizal Fungal Communities in Alpine Meadow of The Qinghai-Tibetan Plateau[J]. Acta Agrestia Sinica, 2024, 32(7): 2054-2061.

国显宝, 韩冰, 逯新民, 何奕成, 于露, 张万通, 段丽辉, 耿依仪, 邵新庆, 俞点, 韩伽怡. 氮添加对青藏高原高寒草甸丛枝菌根真菌群落的影响[J]. 草地学报, 2024, 32(7): 2054-2061.

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The Effects of Nitrogen Addition on Arbuscular Mycorrhizal Fungal Communities in Alpine Meadow of The Qinghai-Tibetan Plateau

氮添加对青藏高原高寒草甸丛枝菌根真菌群落的影响

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