Development of DUS Test Guide for New Bupleurum Chinense Varieties

doi:10.11733/j.issn.1007-0435.2024.10.012

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (10): 3121-3132.DOI: 10.11733/j.issn.1007-0435.2024.10.012

Development of DUS Test Guide for New Bupleurum Chinense Varieties

HE Long-jiao¹, SUN Zhe¹, FAN A-mei¹, DU Xiao-rong¹, LI Ao-xuan¹, LI Jia-jie³, SONG Yun^1,2, QIAO Yong-gang^1,2

1. College of Life Sciences, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Shanxi key lab. for Modernization of TCVM, Taigu, Shanxi Provinc 030801, China;
3. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China

Received:2024-02-23 Revised:2024-04-06 Published:2024-11-04

柴胡植物新品种DUS测试指南的研制

何龙娇¹, 孙哲¹, 樊阿梅¹, 杜晓蓉¹, 李澳旋¹, 李佳杰³, 宋芸^1,2, 乔永刚^1,2

1. 山西农业大学生命科学学院, 山西太谷 030801;
2. 中兽医药现代化山西省重点实验室, 山西太谷 030801;
3. 天津中医药大学中药学院, 天津 301617

通讯作者: 乔永刚,Email:sxndqyg@126.com
作者简介:何龙娇(2000-)，女，汉族，山西运城人，硕士研究生，主要从事药用植物遗传学研究，E-mail：3219915173@qq.com；孙哲(1997-)，男，汉族，山西河津人，硕士研究生，主要从事药用植物遗传学研究，E-mail：297093122@qq.com
基金资助:
山西省基础研究计划自然科学研究面上项目(20210302123390);山西省现代农业中药材产业技术体系项目(2024);山西农业大学科技创新提升工程项目(CXGC2023041)资助

Abstract

Abstract: Bupleurum chinense DC. is a perennial herb of the genus Bupleurum in the Umbelliferae family,and its root can be used as medicine. The germplasm is seriously mixed in the production of Bupleurum chinense,and it is imperative to breed new varieties. In order to improve the DUS (Distinctness,uniformity and stability) test guidelines for Bupleurum chinense,22 samples of Bupleurum chinense from different habitats were collected in this study. The DUS test guidelines for 2-year-old and 3-year-old Bupleurum chinense were developed based on morphological traits,and the traits related to yield and content were added to prepare a more complete draft of DUS test guidelines for Bupleurum chinense medicinal plants in contrast to the original guidelines. A total of 41 traits were selected as the test traits of the DUS test guide for Bupleurum chinense,including 24 basic tested traits and 17 selected traits;there were 0 quality traits,15 false quality traits and 26 quantitative traits. It involved 6 plant traits,4 stem traits,7 leaf traits,7 basal leaf traits,6 flower traits,10 root traits,and 1 growth period trait. This guideline improves the lack of some traits and effective components in the DUS test guideline of Bupleurum chinense,which is of great significance for the breeding of new varieties of Bupleurum chinense in the future.

Key words: Bupleurum chinense, DUS, Characteristics

摘要： 柴胡(Bupleurum chinense DC.)为伞形科柴胡属多年生草本植物，以根入药。柴胡生产中种质混杂较为严重，新品种选育势在必行。为完善柴胡植物新品种特异性、一致性和稳定性(Distinctness，uniformity and stability，DUS)测试指南，本试验收集了22份不同产地柴胡，对二年生与三年生柴胡基于形态学性状开展了DUS测试指南的研制，与原指南相比，增加了根的产量与含量相关的性状，最终确定了41个测试性状，其中包括24个必测的基本性状，17个选测性状；有质量性状0个、假质量性状15个和数量性状26个；涉及到植株性状6个、茎性状4个、叶性状7个、基生叶性状7个、花性状6个、根性状10个、生育期性状1个。本指南为我国柴胡性状测试、新品种的授权提供了支撑，对柴胡新品种选育具有重大意义。

关键词: 柴胡, DUS, 性状

CLC Number:

S567.79

HE Long-jiao, SUN Zhe, FAN A-mei, DU Xiao-rong, LI Ao-xuan, LI Jia-jie, SONG Yun, QIAO Yong-gang. Development of DUS Test Guide for New Bupleurum Chinense Varieties[J]. Acta Agrestia Sinica, 2024, 32(10): 3121-3132.

何龙娇, 孙哲, 樊阿梅, 杜晓蓉, 李澳旋, 李佳杰, 宋芸, 乔永刚. 柴胡植物新品种DUS测试指南的研制[J]. 草地学报, 2024, 32(10): 3121-3132.

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Development of DUS Test Guide for New Bupleurum Chinense Varieties

柴胡植物新品种DUS测试指南的研制

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