Abstract: To investigate the effects of different AMF and inoculation methods on the growth and physiological characteristics of Verbena bonariensis seedlings. We studied four AMF, Claroideoglomus etunicatum （Ce）, Rhizophagus irregularis （Rir）, Rhizophagus intraradices （Ri）, Funneliformis mosseae （Fm） and two methods of inoculation （A D inoculation method involved thorough mixing of inoculum, sand, vermiculite and filling trays with this mixture, and a H inoculation method, in which vermiculite was filled to 2/3 of the tray, inoculated with inoculum and sand, and then covered it with vermiculite） on the growth and physiological indicators of Verbena bonariensis seedlings through tray cultivation experiments. The results showed that the mycorrhizal infection rate, infection density and arbuscule abundance of Verbena bonariensis inoculated with Ce were significantly higher than those of the other AMF. The plant height, total biomass, underground biomass, photochemical reflectance index and mycorrhizal dependence of Ce under the H inoculation method were significantly higher than those under the D inoculation method. Correlation analysis showed that mycorrhizal infection rate, infection density and arbuscule abundance were significantly positively correlated with plant height, total biomass and underground biomass. AMF strains and inoculation methods significantly affected the growth and physiological indicators of plants. The Ce effect under the H inoculation method was better than the others. The research results can provide a theoretical basis for the application of AMF in Verbena bonariensis seedling cultivation and other aspects.

Key words: Arbuscular mycorrhizal fungi, Verbena bonariensis, Seedlings, Growth, Physiological characteristic

摘要： 为探讨不同菌种和接种方式对柳叶马鞭草（Verbena bonariensis）幼苗生长和生理特性的影响。研究了4种丛枝菌根真菌（Arbuscular mycorrhizal fungi,AMF）即幼套球囊霉（Claroideoglomus etunicatum,Ce）、异形根孢囊霉（Rhizophagus irregularis,Rir）、根内根孢囊霉（Rhizophagus intraradices,Ri）、摩西斗管囊霉（Funneliformis mosseae,Fm）、2种接种方式（菌剂、砂子与蛭石混匀的D接种方式和蛭石装至穴盘穴位2/3处,接种菌剂、砂子,再覆蛭石的H接种方式）对柳叶马鞭草幼苗生长和生理的影响。结果表明：Ce接种柳叶马鞭草的菌根侵染率、侵染密度及丛枝丰度显著高于其他菌种。Ce在H接种方式下的株高、总生物量、地下生物量和光化学反射率指数显著高于D接种方式。相关分析表明,菌根侵染率、侵染密度和丛枝丰度与柳叶马鞭草的株高、总生物量和地下生物量呈显著正相关。AMF菌种及接种方式显著影响植物的生长和生理指标,以H接种方式下的Ce效果较好。其研究结果能为AMF在柳叶马鞭草育苗等方面的应用提供理论依据。

关键词: 丛枝菌根真菌, 柳叶马鞭草, 幼苗, 生长, 生理特性