Identification of SnRK2 Gene Family in Psammochloa villosa and Analysis of Their Expression under Drought Stress

doi:10.11733/j.issn.1007-0435.2025.10.005

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3173-3184.DOI: 10.11733/j.issn.1007-0435.2025.10.005

Identification of SnRK2 Gene Family in Psammochloa villosa and Analysis of Their Expression under Drought Stress

YANG Qian^1,2, SU Xu^1,2,3, LIU Yu-ping^1,2,3, LIU Tao⁴, ZHENG Ying-hui^1,2, JIN Jia-rui^1,2, ZHANG Peng-hui^1,2, YU Ming-jun^1,2

1. School of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China;
2. Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Xizang Plateau in Qinghai Province, Qinghai Normal University, Xining, Qinghai Province 810008, China;
3. Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, Qinghai Province 810016, China;
4. Qinghai Provincial Key Laboratory of Plateau Climate Change and Corresponding Ecological and Environmental Effects, Qinghai Institute of Technology, Xining, Qinghai Province 810016, China

Received:2025-01-15 Revised:2025-02-20 Published:2025-10-17

沙鞭SnRK2基因家族鉴定及干旱胁迫下表达分析

杨倩^1,2, 苏旭^1,2,3, 刘玉萍^1,2,3, 刘涛⁴, 郑莹晖^1,2, 靳佳瑞^1,2, 张朋辉^1,2, 余明君^1,2

1. 青海师范大学生命科学学院, 青海西宁 810008;
2. 青海师范大学青海省青藏高原生物多样性形成机制与综合利用重点实验室, 青海西宁 810008;
3. 青海师范大学高原科学与可持续发展研究院, 青海西宁 810016;
4. 青海理工学院青海省高原气候变化及其生态环境效应重点实验室, 青海西宁 810016

通讯作者: 刘玉萍,E-mail:lyp8527970@126.com
作者简介:刘玉萍,E-mail:lyp8527970@126.com
基金资助:
国家自然科学基金项目（32160297）；青海省重点研发与转化计划-国际合作专项（2023-HZ-810）资助

Abstract

Abstract: In order to clarify the gene function and mechanism of SnRK2 protein kinases in Psammochloa villosa， we identified the family member of SnRK2 at the genomic level adopting the bioinformatics method， and analyzed their expression patterns under drought stress using real-time quantitative PCR. The results showed that a total of ten gene family members of SnRK2 were identified in the genome of P<. villosa， which were named as PvSnRK2.1-PvSnRK2.10， and separately distributed on ten chromosomes. The number of amino acid residues of PvSnRK2 proteins ranged from 341 to 496， with molecular masses between 38 636.17 from 56 572.38 kD， and the isoelectric points were from 4.71 to 8.81， all of which were hydrophilic proteins. The PvSnRK2 gene family could be divided into three subfamilies， whose exon number was mainly eight. Three PvSnRK2 genes had the collinearity relationships within species. The promoters of PvSnRK2 gene family members contained various hormone-related and abiotic stress responsive elements. PvSnRK2 proteins might have interaction relationships with bZIP， PP2C and ABF proteins. Besides， there were differences in expression characteristics during different tissues of these PvSnRK2 genes. This study provided a theoretical basis for screening more drought-resistant key genes from P<. villosa in the future.

Key words: Psammochloa villosa, SnRK2, Protein kinase, Bioinformatics, Drought stress, Expression pattern

摘要： 为了明晰沙鞭SnRK2蛋白激酶的基因功能和作用机制，本研究采用生物信息学方法从基因组水平鉴定SnRK2家族成员，利用实时荧光定量PCR反应分析干旱胁迫下的表达模式。结果表明：沙鞭基因组中共鉴定到10个SnRK2基因家族成员，命名为PvSnRK2.1~PvSnRK2.10，分别分布于10条染色体上；PvSnRK2蛋白氨基酸残基数为341~496，分子质量为38 636.17~56 572.38 kD，等电点为4.71~8.81，均为亲水性蛋白；PvSnRK2基因家族可被划分为3个亚族，外显子数量以9个为主，3条PvSnRK2基因物种内存在共线性关系；PvSnRK2基因家族成员启动子含有多种激素类和逆境胁迫响应类作用元件；PvSnRK2蛋白可能与bZIP，PP2C和ABF三种蛋白具有互作关系；PvSnRK2基因家族成员在不同组织中的表达存在差异。研究结果为将来筛选沙鞭中更多的耐旱关键基因提供了理论依据。

关键词: 沙鞭, SnRK2, 蛋白激酶, 生物信息学, 干旱胁迫, 表达模式

CLC Number:

Q945.78

YANG Qian, SU Xu, LIU Yu-ping, LIU Tao, ZHENG Ying-hui, JIN Jia-rui, ZHANG Peng-hui, YU Ming-jun. Identification of SnRK2 Gene Family in Psammochloa villosa and Analysis of Their Expression under Drought Stress[J]. Acta Agrestia Sinica, 2025, 33(10): 3173-3184.

杨倩, 苏旭, 刘玉萍, 刘涛, 郑莹晖, 靳佳瑞, 张朋辉, 余明君. 沙鞭SnRK2基因家族鉴定及干旱胁迫下表达分析[J]. 草地学报, 2025, 33(10): 3173-3184.

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Identification of SnRK2 Gene Family in Psammochloa villosa and Analysis of Their Expression under Drought Stress

沙鞭SnRK2基因家族鉴定及干旱胁迫下表达分析

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