Gene Editing Technology Development and Its Application in Turfgrass Species

doi:10.11733/j.issn.1007-0435.2025.12.001

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (12): 3859-3873.DOI: 10.11733/j.issn.1007-0435.2025.12.001

• 2025-12-28 •

Gene Editing Technology Development and Its Application in Turfgrass Species

YANG Xin-yu¹, HU Tao², XU Bin³, XU Li-xin¹, HAN Lie-bao¹

1. College of Grassland Science, Beijing Forestry University, Beijing 100083, China;
2. College of Pastoral Agriculture Science and Technology, Lanzhou, Gansu Province 730020, China;
3. College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China

Received:2024-12-20 Revised:2025-03-04 Published:2025-12-01

基因编辑技术在草坪草中的应用

杨馨雨¹, 胡涛², 徐彬³, 许立新¹, 韩烈保¹

1. 北京林业大学草业与草原学院, 北京 100083;
2. 兰州大学草地农业科技学院, 甘肃兰州 730020;
3. 南京农业大学草业学院, 江苏南京 210095

通讯作者: 韩烈保，E-mail：hanliebao@163.com
作者简介:杨馨雨（2000-），女，汉族，甘肃兰州人，硕士研究生，主要从事草地植物栽培与育种研究，E-mail：xinyuyang_yxy07@163.com；
基金资助:
国家重点研发计划（2023YFD1200302）资助

Abstract

Abstract: Gene editing technology can rapidly realize precise modification of genes to obtain stable inheritance of excellent traits. With the advancement of science and technology， the research and application of gene editing has expanded to a wider field. This paper provided a brief introduction to the principles of different gene editing technologies， focused on the development and application of CRISPR/Cas technology as well as the research progress of this technology in the field of turfgrass， and gave a systematic description of the status of the regulatory system of gene edited plants. Finally， the application and development of gene editing technology in turfgrass was discussed.

Key words: Gene editing, CRISPR/Cas9, Herbaceous plant, Turfgrass

摘要： 基因编辑技术能够精准修饰目标基因，使目标生物获得可稳定遗传的优良性状。随着科学和技术的进步，基因编辑技术的应用拓展到更宽广的领域。本文首先对不同基因编辑技术作了概述，重点介绍了CRISPR/Cas技术的发展与应用以及该技术在草类植物尤其是草坪草中的研究进展，对基因编辑植物监管制度现状做了系统阐述，并对基因编辑技术在草坪草中的应用和发展做了讨论和展望。

关键词: 基因编辑, CRISPR/Cas9, 草类植物, 草坪草

CLC Number:

Q789

YANG Xin-yu, HU Tao, XU Bin, XU Li-xin, HAN Lie-bao. Gene Editing Technology Development and Its Application in Turfgrass Species[J]. Acta Agrestia Sinica, 2025, 33(12): 3859-3873.

杨馨雨, 胡涛, 徐彬, 许立新, 韩烈保. 基因编辑技术在草坪草中的应用[J]. 草地学报, 2025, 33(12): 3859-3873.

References

[1] PUCHTA H. The repair of double-strand breaks in plants： mechanisms and consequences for genome evolution[J]. Journal of Experimental Botany， 2005，56（409）：1-14
[2] CRISTEA S， FREYVERT Y， SANTIAGO Y， et al. In vivo cleavage of transgene donors promotes nuclease‐mediated targeted integration[J]. Biotechnology and Bioengineering， 2013， 110（3）： 871-880
[3] MARESCA M， LIN V G， GUO N， et al. Obligate Ligation-Gated Recombination （ObLiGaRe）： Custom-designed nuclease-mediated targeted integration through nonhomologous end joining[J]. Genome Research， 2013，23（3）：539-546
[4] BORTESI L， FISCHER R. The CRISPR/Cas9 system for plant genome editing and beyond[J]. Biotechnology Advances， 2015，33（1）：41-52
[5] LIU Y G，LI G S，ZHANG Y L，et al. Current advances on CRISPR/Cas genome editing technologies in plants[J]. Journal of South China Agricultural University，2019，40（5）：38-49 刘耀光，李构思，张雅玲，等. CRISPR/Cas植物基因组编辑技术研究进展[J]. 华南农业大学学报， 2019，40（5）：38-49
[6] OSAKABE Y， OSAKABE K. Genome Editing with Engineered Nucleases in Plants[J]. Plant and Cell Physiology， 2015，56（3）：389-400
[7] WANG H， LA RUSSA M， QI L S. CRISPR/Cas9 in Genome Editing and Beyond[J]. Annual Review of Biochemistry， 2016，85（1）：227-264
[8] SHAN Q， WANG Y， LI J， et al. Targeted genome modification of crop plants using a CRISPR-Cas system[J]. Nature Biotechnology， 2013，31（8）：686-688
[9] LI J， MENG X， ZONG Y， et al. Gene replacements and insertions in rice by intron targeting using CRISPR–Cas9[J]. Nature Plants， 2016，2（10）：16139
[10] NEKRASOV V， STASKAWICZ B， WEIGEL D， et al. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease[J]. Nature Biotechnology， 2013，31（8）：691-693
[11] XIE K， YANG Y. RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System[J]. Molecular Plant， 2013，6（6）：1975-1983
[12] ZHAO H， ZHAO S， CAO Y， et al. Development of a single transcript CRISPR/Cas9 toolkit for efficient genome editing in autotetraploid alfalfa[J]. The Crop Journal，2024，12（3）：788-795
[13] LI L J，ZHANG N，ZHANG Z W，et al. ZjSGR gene editing of Zoysia Japonica using the CRISPR/Cas9 system[J]. Pratacultural Science，2020，37（5）：864-871 李丽菁，张娜，张智韦，等. 利用CRISPR/Cas9系统编辑日本结缕草ZjSGR基因技术研究[J]. 草业科学， 2020，37（5）：864-871
[14] PORTEUS M H， CARROLL D. Gene targeting using zinc finger nucleases[J]. Nature Biotechnology， 2005，23（8）：967-973
[15] BITINAITE J， WAH D A， AGGARWAL A K， et al. Fok I dimerization is required for DNA cleavage[J]. Proceedings of the National Academy of Sciences， 1998，95（18）：10570-10575
[16] WU J， KANDAVELOU K， CHANDRASEGARAN S. Custom-designed zinc finger nucleases： What is next [J]. Cellular and Molecular Life Sciences， 2007，64（22）：2933-2944
[17] KIM Y G， CHA J， CHANDRASEGARAN S. Hybrid restriction enzymes： zinc finger fusions to Fok I cleavage domain.[J]. Proceedings of the National Academy of Sciences， 1996，93（3）：1156-1160
[18] DEFRANCESCO L. Move over ZFNs： a new technology for genome editing may put the zinc finger nuclease franchise out of business， some believe. Not so fast， say the finger people[J]. Nature Biotechnology， 2011，29（8）：681-685
[19] MOSCOU M J， BOGDANOVE A J. A Simple Cipher Governs DNA Recognition by TAL Effectors[J]. SCIENCE， 2009，326（5959）：1501-1501
[20] BOCH J， SCHOLZE H， SCHORNACK S， et al. Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors[J]. SCIENCE， 2009，326（5959）：1509-1512
[21] CHANDRASEGARAN S， CARROLL D. Origins of Programmable Nucleases for Genome Engineering[J]. Journal of Molecular Biology， 2016，428（5）：963-989
[22] GUPTA R M， MUSUNURU K. Expanding the genetic editing tool kit： ZFNs， TALENs， and CRISPR-Cas9[J]. The Journal of clinical investigation， 2014，124（10）：4154-4161
[23] GODDE J S， BICKERTON A. The Repetitive DNA Elements Called CRISPRs and Their Associated Genes： Evidence of Horizontal Transfer Among Prokaryotes[J]. Journal of Molecular Evolution， 2006，62（6）：718-729
[24] SEED K D， LAZINSKI D W， CALDERWOOD S B， et al. A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity[J]. Nature， 2013，494（7438）：489-491
[25] KOONIN E V， MAKAROVA K S， ZHANG F. Diversity， classification and evolution of CRISPR-Cas systems[J]. Current Opinion in Microbiology， 2017，37：67-78
[26] ZHAN X， LU Y， ZHU J， et al. Genome editing for plant research and crop improvement[J]. Journal of Integrative Plant Biology， 2021， 63（1）： 3-33
[27] MAHFOUZ M M， PIATEK A， STEWART C N. Genome engineering via TALENs and CRISPR/Cas9 systems： challenges and perspectives[J]. Plant Biotechnology Journal， 2014，12（8）：1006-1014
[28] BARRANGOU R. CRISPR‐Cas systems and RNA‐guided interference[J]. WIREs RNA， 2013，4（3）：267-278
[29] GASIUNAS G， BARRANGOU R， HORVATH P， et al. Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria[J]. Proceedings of the National Academy of Sciences， 2012，109（39）：E2579-E2586
[30] JINEK M， CHYLINSKI K， FONFARA I， et al. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity[J]. Science， 2012，337（6096）：816-821
[31] QI L S， LARSON M H， GILBERT L A， ET AL. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell， 2013，152（5）：1173-1183
[32] TYCKO J， MYER V E， HSU P D. Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity[J]. Molecular Cell， 2016，63（3）：355-370
[33] ZETSCHE B， GOOTENBERG J S， ABUDAYYEH O O， et al. Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System[J]. Cell， 2015，163（3）：759-771
[34] LI S， ZHANG X， WANG W， et al. Expanding the Scope of CRISPR/Cpf1-Mediated Genome Editing in Rice[J]. Molecular Plant， 2018，11（7）：995-998
[35] ANZALONE A V， RANDOLPH P B， DAVIS J R， et al. Search-and-replace genome editing without double-strand breaks or donor DNA[J]. Nature， 2019，576（7785）：149-157
[36] ABUDAYYEH O O， GOOTENBERG J S， ESSLETZBICHLER P， et al. RNA targeting with CRISPR–Cas13[J]. Nature， 2017，550（7675）：280-284
[37] COX D B T， GOOTENBERG J S， ABUDAYYEH O O， et al. RNA editing with CRISPR-Cas13[J]. 2017，358（6366）：1019-1027
[38] KONERMANN S， LOTFY P， BRIDEAU N J， et al. Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors[J]. Cell， 2018，173（3）：665-676
[39] GAUDELLI N M， KOMOR A C， REES H A， et al. Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage[J]. Nature， 2017，551（7681）：464-471
[40] KOMOR A C， KIM Y B， PACKER M S， et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage[J]. Nature， 2016，533（7603）：420-424
[41] LI C， ZONG Y， WANG Y， et al. Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion[J]. Genome Biology， 2018（19）：1-9
[42] KANTOR A， MCCLEMENTS M E， MACLAREN R E. CRISPR-Cas9 DNA base-editing and prime-editing[J]. International journal of molecular sciences， 2020，21（17）：6240
[43] YANG L， ZHANG X， WANG L， et al. Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants[J]. Protein & cell， 2018， 9（9）： 814-819
[44] XIE J， HUANG X， WANG X， et al. ACBE， a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems[J]. BMC Biology， 2020（18）：1-14
[45] KURT I C， ZHOU R， IYER S， et al. CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells[J]. Nature Biotechnology， 2021，39（1）：41-46
[46] ZHAO D， LI J， LI S， et al. Glycosylase base editors enable C-to-A and C-to-G base changes[J]. Nature Biotechnology， 2021，39（1）：35-40
[47] TONG H， LIU N， WEI Y， et al. Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase[J]. National Science Review， 2023， 10（8）： nwad143
[48] ANZALONE A V， GAO X D， Podracky C J， et al. Programmable deletion， replacement， integration and inversion of large DNA sequences with twin prime editing[J]. Nature biotechnology， 2022，40（5）：731-740
[49] SUN C， LEI Y， LI B， et al. Precise integration of large DNA sequences in plant genomes using PrimeRoot editors[J]. Nature Biotechnology， 2024，42（2）：316-327
[50] YARNALL M T N， IOANNIDI E I， SCHMITT-ULMS C， et al. Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases[J]. Nature Biotechnology， 2023，41（4）：500-512
[51] VILLIGER L， JOUNG J， KOBLAN L， et al. CRISPR technologies for genome， epigenome and transcriptome editing[J]. Nature Reviews Molecular Cell Biology，2024：1-24
[52] AWAN M J A， MAHMOOD M A， NAQVI R Z， et al. PASTE： a high-throughput method for large DNA insertions[J]. Trends in Plant Science， 2023，28（5）：509-511
[53] STRECKER J， LADHA A， GARDNER Z， et al. RNA-guided DNA insertion with CRISPR-associated transposases[J]. Science， 2019，365（6448）：48-53
[54] KACZMARSKA Z， CZARNOCKI-CIECIURA M， GÓRECKA-MINAKOWSKA K M， et al. Structural basis of transposon end recognition explains central features of Tn7 transposition systems[J]. Molecular cell， 2022，82（14）：2618-2632
[55] WEI J， LI Y. CRISPR-based gene editing technology and its application in microbial engineering[J]. Engineering Microbiology， 2023，3（4）：100101
[56] CHENG Z H， WU J， LIU J Q， et al. Repurposing CRISPR RNA-guided integrases system for one-step， efficient genomic integration of ultra-long DNA sequences[J]. Nucleic Acids Research， 2022，50（13）：7739-7750
[57] ISHINO Y， SHINAGAWA H， MAKINO K， et al. Nucleotide sequence of the iap gene， responsible for alkaline phosphatase isozyme conversion in Escherichia coli， and identification of the gene product[J]. Journal of Bacteriology， 1987，169（12）：5429-5433
[58] LI J F， NORVILLE J E， AACH J， et al. Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9[J]. Nature Biotechnology， 2013，31（8）：688-691
[59] ZHANG Y， BAI Y， WU G， et al. Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat[J]. Plant Journal， 2017，91（4）：714-724
[60] LUO M， GILBERT B， AYLIFFE M. Applications of CRISPR/Cas9 technology for targeted mutagenesis， gene replacement and stacking of genes in higher plants[J]. Plant Cell Reports， 2016，35（7）：1439-1450
[61] ČERMÁK T， BALTES N J， ČEGAN R， et al. High-frequency， precise modification of the tomato genome[J]. Genome Biology， 2015，16（1）：232
[62] SVITASHEV S， YOUNG J K， SCHWARTZ C， et al. Targeted mutagenesis， precise gene editing， and site-specific gene insertion in maize using Cas9 and guide RNA[J]. Plant Physiology， 2015，169（2）：931-945
[63] LI Z， LIU Z B， XING A， et al. Cas9-Guide RNA Directed Genome Editing in Soybean[J]. Plant Physiology， 2015，169（2）：960-970
[64] BUTT H， EID A， ALI Z， et al. Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule[J]. Frontiers in Plant Science， 2017，8：1441
[65] WATANABE K， ODA-YAMAMIZO C， SAGE-ONO K， et al. Alteration of flower colour in Ipomoea nil through CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase 4[J]. Transgenic Research， 2018，27（1）：25-38
[66] TONG C， WU F， YUAN Y， et al. High‐efficiency CRISPR /Cas‐based editing of Phalaenopsis orchid MADS genes[J]. Plant Biotechnology Journal， 2020，18（4）：889-891
[67] MICHNO J M， WANG X， LIU J， et al. CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme[J]. GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN， 2015，6（4）：243-252
[68] MENG Y， HOU Y， WANG H， et al. Targeted mutagenesis by CRISPR/Cas9 system in the model legume Medicago truncatula[J]. Plant Cell Reports， 2017，36（2）：371-374
[69] DU S M. Functional analysis of small GTP-binding proteins activating protein MtArfGAP in Medicago Truncatula[D]. Guangzhou：South China Agricultural University，2017：37 杜思梦. 截形苜蓿MtArfGAP功能的初步研究[D]. 广东：华南农业大学， 2017：37
[70] ZHOU C C. Cloning of MtCOMT gene，construction of CRISPR/Cas9 vector and transformation of Medicago truncatula[D]. Harbin：Harbin Normal University，2020：37 周嫦嫦. MtCOMT基因克隆、CRISPR/Cas9载体构建及转化蒺藜苜蓿的研究[D]. 哈尔滨：哈尔滨师范大学， 2020：37
[71] YE Q， MENG X， CHEN H， et al. Construction of genic male sterility system by CRISPR/Cas9 editing from model legume to alfalfa[J]. Plant Biotechnology Journal， 2022，20（4）：613-615
[72] LEBEDEVA M A， DOBYCHKINA D A， LUTOVA L A. CRISPR/Cas9-mediated knock-out of the MtCLE35 gene highlights its key role in the control of symbiotic nodule numbers under high-nitrate conditions[J]. International Journal of Molecular Sciences， 2023，24（23）：16816
[73] GÜNGÖR B， BIRÓ J B， DOMONKOS Á， et al. Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich （NCR） genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system[J]. Scientific Reports， 2023，13（1）：20676
[74] GAO R， FEYISSA B A， CROFT M， et al. Gene editing by CRISPR/Cas9 in the obligatory outcrossing Medicago sativa[J]. Planta， 2018，247（4）：1043-1050
[75] WOLABU T W， CONG L， PARK J J， et al. Development of a highly efficient multiplex genome editing system in outcrossing tetraploid alfalfa （Medicago sativa）[J]. Frontiers in Plant Science， 2020，11：1063
[76] WOLABU T W， MAHMOOD K， JEREZ I T， et al. Multiplex CRISPR/Cas9‐mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 （ MsFTa1 ） leads to delayed flowering time with improved forage biomass yield and quality[J]. Plant Biotechnology Journal， 2023，21（7）：1383-1392
[77] SINGER S D， BURTON HUGHES K， SUBEDI U， et al. The CRISPR/Cas9-Mediated modulation of Squamosa Promoter-Binding Protein-Like 8 in alfalfa leads to distinct phenotypic outcomes[J]. Frontiers in Plant Science， 2022，12：774146
[78] ZHENG L， WEN J， LIU J， et al. From model to alfalfa： Gene editing to obtain semidwarf and prostrate growth habits[J]. The Crop Journal， 2022，10（4）：932-941
[79] LI L，XU M Z，LIU Y R，et al. Codon bias analysis of Medicago genome[J]. Acta Agrestia Sinica，2024，23（9）：2695-2706 李琳，徐明志，刘燕蓉，等.苜蓿基因组密码子偏好性分析[J].草地学报，2024，23（9）：2695-2706
[80] PARK J J， YOO C G， FLANAGAN A， et al. Defined tetra-allelic gene disruption of the 4-coumarate：coenzyme A ligase 1 （Pv4CL1） gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release[J]. Biotechnology for Biofuels， 2017，10（1）：284
[81] QIU R，HE F，LI R，et al. Highly efficient gene editing of lignin gene F5H in switchgrass[J]. Chinese Bulletin of Botany，2023，58（2）：298-307 邱锐，何峰，李瑞，等.柳枝稷木质素基因F5H的高效编辑[J].植物学报，2023，58（2）：298-307
[82] LIU Y， MERRICK P， ZHANG Z， et al. Targeted mutagenesis in tetraploid switchgrass （ Panicum virgatum L.） using CRISPR/Cas9[J]. Plant Biotechnology Journal， 2018，16（2）：381-393
[83] LIU Y， WANG W， YANG B， et al. Functional Analysis of the teosinte branched 1 Gene in the Tetraploid Switchgrass （Panicum virgatum L.） by CRISPR/Cas9-Directed Mutagenesis[J]. Frontiers in Plant Science， 2020，11：572193
[84] TENG K， CHANG Z H， XIAO G Z， et al. Molecular cloning and characterization of a chlorophyll degradation regulatory gene （ZjSGR） from Zoysia japonica[J]. Genetics and Molecular Research， 2016，15（2）：8176
[85] ZHANG R. Study on CRISPR/Cas9-mediated DNA-free gene editing of ZjSGR in Zoysia Japonica[D]. Beijing：Beijing Forestry University，2019：10-20 张睿. CRISPR/Cas9介导无外源DNA日本结缕草ZjSGR基因编辑技术研究[D]. 北京：北京林业大学，2019：10-20
[86] MAO Y Y，LI L J，LI J C，et al. Gene functional analysis of ZjSGR gene in Zoysia Japonica leaves in response to vitro stress[J]. Acta Agrestia Sinica，2022，30（6）：1396-1402 茅远煜，李丽菁，李进超，等.结缕草ZjSGR基因在离体模拟逆境胁迫环境下的功能[J].草地学报，2022，30（6）：1396-1402
[87] NG H M， GONDO T， TANAKA H， et al. CRISPR/Cas9-mediated knockout of NYC1 gene enhances chlorophyll retention and reduces tillering in Zoysia matrella （L.） Merrill[J]. Plant Cell Reports， 2024，43（2）：50
[88] ZHANG Y， RAN Y， NAGY I， et al. Targeted mutagenesis in ryegrass （ Lolium spp.） using the CRISPR/Cas9 system[J]. Plant Biotechnology Journal， 2020，18（9）：1854-1856
[89] JIANG Q Q. Construction of CRISPR/Cas9 targeted editing vectors for LpCKX1 gene and the establishment of genetic transformation system in Lolium perenne L[D]. Yantai：Yantai University，2020：66-67 姜倩倩. 多年生黑麦草LpCKX1基因CRISPR/Cas9靶向编辑载体的构建及遗传转化体系的建立[D]. 烟台：烟台大学， 2020：66-67
[90] KUMAR R， KAMUDA T， BUDHATHOKI R， et al. Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass[J]. Frontiers in Genome Editing， 2022， 4：960414
[91] YAO J M，HAO H H，ZHANG J，et al. The use of the tRNA-sgRNA/Cas9 system for gene editing in perennial ryegrass protoplasts[J]. Acta Prataculturae Sinica，2023，32（4）：129-141 姚佳明，郝欢欢，张敬， et al. tRNA-sgRNA/Cas9系统介导多年生黑麦草原生质体的基因编辑[J]. 草业学报， 2023，32（4）：129-141
[92] ZHANG L， WANG T， WANG G， et al. Simultaneous gene editing of three homoeoalleles in self‐incompatible allohexaploid grasses[J]. Journal of Integrative Plant Biology， 2021，63（8）：1410-1415
[93] BI A， WANG T， WANG G， et al. Stress memory gene FaHSP17.8-CII controls thermotolerance via remodeling PSII and ROS signaling in tall fescue[J]. Plant Physiology， 2021，187（3）：1163-1176
[94] MAY D， SANCHEZ S， GILBY J， et al. Multi-allelic gene editing in an apomictic， tetraploid turf and forage grass （Paspalum notatum Flüggé） using CRISPR/Cas9[J]. Frontiers in Plant Science， 2023，14：1225775
[95] ZHANG D B，LUO Y，CHEN W J. Current development of gene editing[J]. Chinese Journal of Biotechnology，2020，36（11）：2345-2356 章德宾，罗瑶，陈文进. 基因编辑技术发展现状[J]. 生物工程学报， 2020， 36（11）：2345-2356
[96] JIAO Y，WU G，HUANG Y H，et al. Genome editing technology and its safety evaluation management[J]. Journal of Agricultural Science and Technology，2018，20（4）：12-19 焦悦，吴刚，黄耀辉，等. 基因组编辑技术及其安全评价管理[J]. 中国农业科技导报， 2018，20（4）：12-19
[97] LIU X G，QIAN B X，SUN J Q，et al. Research progress and prospects of maize gene editing[J]. Journal of Jilin Agricultural University，2025，47（2）：191-201 刘相国，钱步轩，孙佳琪，等. 玉米基因编辑研究进展和前景展望[J]. 吉林农业大学学报，2025，47（2）：191-20
[98] LI X. Research on legal regulation of agricultural gene editing organisms[D]. Wuhan：Huazhong Agricultural University，2023：45-46 李欣. 农业基因编辑生物法律规制问题研究[D]. 武汉：华中农业大学， 2023：45-46

Gene Editing Technology Development and Its Application in Turfgrass Species

基因编辑技术在草坪草中的应用

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[1]	ZHANG Li, YANG Xin-guo, WANG Lei, ZHANG Xue, QU Wen-jie, LIU Rong-guo, ZHANG Bo. Spatial Associations Analysis of Species in Sand-Fixing Community of Caragana korshinskii Based on Point Pattern [J]. Acta Agrestia Sinica, 2025, 33(6): 1886-1893.
[2]	LUO Lai-kai, YIN Ming-yue, ZHU Ling, CHENG Ying, YANG Yan-fang, TAN Kai, ZHAO Kai. Herbaceous Plant Diversity and Environmental Interpretation in Different Habitats： A Case Study of The Anqing Section of The Yangtze River [J]. Acta Agrestia Sinica, 2025, 33(6): 1934-1946.
[3]	WANG Xiu-hong, ZHU Xiao-tian, ZHANG Hui-hui, LIU Zhen-ling, WANG Bao-ping, ZHOU Jing, ZHANG Ji-tao, SHI Xiang-yuan. Effects of the Thickness of Biogas Residue Substrate on the Formation and Transplanting Effect of Tall Tescue Sod [J]. Acta Agrestia Sinica, 2025, 33(5): 1686-1693.
[4]	LI Lin, XU Ming-zhi, LIU Yan-rong, ZHANG Wan-jun. Codon Bias Analysis of Medicago Genome [J]. Acta Agrestia Sinica, 2024, 32(9): 2695-2706.
[5]	MAO Jia-jing, LU Shuang, FEI Xing-yu, DU Yang, XU Li-xin. Clone and Functional Analysis of CiGL2 Gene in Cichorium intybus [J]. Acta Agrestia Sinica, 2024, 32(8): 2366-2373.
[6]	SONG Ge, DENG Ya-nan, LI Ya-ning, YUE Fu-sen, YAN Xue, YAN Shan-chun. Allelopathic Effects of Essential Oil from Quercus mongolica Deciduous Leaves on Seed Germination and Seedling Growth of Four Herbaceous Plants [J]. Acta Agrestia Sinica, 2024, 32(11): 3522-3529.
[7]	XU Hong-da, HE Wei-hai, ZHONG Xiu-juan, LU Yao-dong, CHEN Yong, ZHANG Ju-ming, LIU Tian-zeng. Study on the Response Mechanism of ‘Lanyin No. III’ Zoysiagrass to Irrigation and Fertilizer Coupling [J]. Acta Agrestia Sinica, 2024, 32(10): 3252-3261.
[8]	ZHANG Zhao, NIE Yu-ting, CUI Kai-lun, LYU Yan-zhen, YAN Hui-fang. Research Progress on the Function of Melatonin in Regulating Growth, Development and Stress Resistance in Herbaceous Species [J]. Acta Agrestia Sinica, 2023, 31(9): 2571-2581.
[9]	MA Cheng-ze, CUI Hui-ting, HU Qian-nan, WANG Lu-yu, JIA Fang, WANG Chu, QIAO Jia-yue, LI Yue, SUN Yan. Research Progress on Regeneration System of Turfgrass [J]. Acta Agrestia Sinica, 2023, 31(8): 2241-2252.
[10]	HU Guang-de, ZHOU Ji-qiang, DA Jun-shan, LEI Long-ju, ZHAO Chang-xing, LI Chao-nan, ZHOU Zi-qiang, LIU Jin-rong. Relationship Between Understory Plant Diversity and Soil Properties of Different Forests in Sandy Land of Jinta [J]. Acta Agrestia Sinica, 2023, 31(6): 1834-1841.
[11]	ZHANG Lin, QI Shi, ZHOU Piao, WU Bin-chen, ZHANG Dai, CUI Ran-ran, HUANG Xian, MA Ning. Analysis of Influencing Factors on the Understory Herbaceous Plant Diversity of Platycladus orientalis Forest in Beijing Mountainous Areas [J]. Acta Agrestia Sinica, 2022, 30(8): 2199-2206.
[12]	LIU Xi-ran, LI Zhi-hong, JIANG Lu, NIE Ying-bin. Study on the Synchronization between Rapid Improvement of Soda Alkaline Soil and Lawn Planting [J]. Acta Agrestia Sinica, 2022, 30(12): 3302-3307.
[13]	XU Zheng, XU Qian, LIU Ming-xi, XIANG Zuo-xiang. Effect of ‘Henace’ Herbicide on the Spring Transition of Zoysia japonica ‘Lantai No.3’ Overseeding Turfgrass [J]. Acta Agrestia Sinica, 2021, 29(3): 603-609.
[14]	LI Yu-ying, LIU Na, JING Ze-yao, WEN Wei, CAO Lei, XIA Fang-shan, ZHU hui-sen. Effect of Extract from Artemisia annua on Seed Germination and Seedling Growth of Four Species of Turfgrass [J]. Acta Agrestia Sinica, 2020, 28(5): 1285-1293.
[15]	CUI Hui-ting, SUN Xi-nuo, MA Cheng-ze, HU Qian-nan, SUN Yan. Advances in Applications of Metabolomics Technology in Stress Resistance of Forage and Turfgrass [J]. Acta Agrestia Sinica, 2020, 28(4): 873-880.