The Effects of MgSO4 Stress on the Physiological Responses and Gene Expression of Alfalfa

doi:10.11733/j.issn.1007-0435.2025.12.008

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (12): 3944-3957.DOI: 10.11733/j.issn.1007-0435.2025.12.008

• 2025-12-28 • Previous Articles

The Effects of MgSO₄ Stress on the Physiological Responses and Gene Expression of Alfalfa

WEI Xiao-na¹, ZHANG Xu-ze^1,3, SHEN Ying-fang^1,2,3

1. The College of Ecological Enviroment and Resources, Qinghai Minzu University, Xining, Qinghai Province 810007, China;
2. Key Laboratory of Resource Chemistry and Eco-environmental Protection in Qinghai-Tibet Plateau (Qinghai Minzu University), State Ethnic Affairs Commission, Xining, Qinghai Province 810007, China;
3. Qinghai Provincial Key Laboratory of High value Utilization of Characteristic Economic Plants, Xining, Qinghai Province 810007, China

Received:2024-10-14 Revised:2024-12-13 Published:2025-12-01

MgSO₄胁迫对紫花苜蓿生理响应及基因表达的影响

魏晓娜¹, 张湑泽^1,3, 沈迎芳^1,2,3

1. 青海民族大学生态环境与资源学院, 青海西宁 810007;
2. 国家民委青藏高原资源化学与生态环境保护重点实验室, 青海西宁 810007;
3. 青海省特色经济植物高值化利用重点实验室, 青海西宁 810007

通讯作者: 沈迎芳，E-mail：syfnc@126.com
作者简介:魏晓娜（1998-），女，汉族，山东淄博人，硕士研究生，主要从事药用植物资源学研究，E-mail：1697652839@qq.com；
基金资助:
青海自然科学基金（2025-ZJ-717）；青海省昆仑英才“中青年科技人才”托举项目（2023QHSKXRCTJ28）；青海民族大学2023年度校级规划项目（23GH12）；青海民族大学2024年研究生创新项目（54M2024003）；青海民族大学产教融合研究生联合培养基地建设项目；2024年校级教学成果培育项目资助

Abstract

Abstract: To investigate the effects of MgSO₄ stress on the physiological responses and gene expression of alfalfa （Medicago sativa L.）， the ‘Zhongmu No.1’ alfalfa variety was used as the test material in this study. A hydroponic method was employed to simulate salt stress treatments by setting up six concentrations of MgSO₄ （0， 40， 80， 120， 160， and 200 mmol·L^-1）. The germination indices of alfalfa seeds， the growth and physiological indices of seedlings， and the expression levels of salt-tolerance-related genes were determined. The results showed that as the concentration of MgSO₄ increased， the germination and growth of alfalfa seedlings were inhibited， the contents of chlorophyll， proline， soluble protein， soluble sugar， malondialdehyde， and the activity of superoxide dismutase in seedlings were increased. The root-shoot ratio and the activities of peroxidase and catalase was first increased and then decreased. By analyzing of the expression levels of salt-tolerance-related genes in alfalfa， it was found that genes related to enzyme activity， photosynthesis， flavonol synthesis， wax synthesis， and brassinolide synthesis pathways may play important roles in the tolerance of ‘Zhongmu No.1’ alfalfa seedlings to MgSO₄ stress. In conclusion， ‘Zhongmu No.1’ alfalfa has the potential to be cultivated on saline-alkali land with MgSO₄ content less than or equal to 200 mmol·L^-1 and pH less than 9.0.

Key words: MgSO₄, Germination of seeds, Growth and physiological indicators, Expression of genes

摘要： 为研究MgSO₄胁迫对紫花苜蓿（Medicago sativa L.）生理响应和基因表达的影响，本研究以紫花苜蓿 ‘中苜1号’为供试材料，利用水培法，设置6个浓度（0，40，80，120，160和200 mmol·L^-1）MgSO₄模拟盐胁迫处理，测定紫花苜蓿种子萌发指标、幼苗生长及生理指标和耐盐相关基因的表达量。结果表明：随着MgSO₄浓度升高，紫花苜蓿种子萌发和幼苗生长受到抑制；幼苗内叶绿素、脯氨酸、可溶性蛋白、可溶性糖、丙二醛含量和超氧化歧化酶活性呈上升趋势；根冠比、过氧化物酶和过氧化氢酶活性呈先上升后下降趋势；通过分析紫花苜蓿耐盐相关基因表达量变化发现，与酶活、光合作用、黄酮醇合成、蜡质合成和油菜素内酯合成途径相关的基因在‘中苜1号’紫花苜蓿幼苗耐MgSO₄胁迫中可能发挥重要作用。综上，‘中苜1号’紫花苜蓿具有在MgSO₄含量小于等于200 mmol·L^-1、pH值小于9的盐碱地上种植的潜力。

关键词: MgSO₄, 种子萌发, 生长及生理指标, 基因表达

CLC Number:

S541.9

WEI Xiao-na, ZHANG Xu-ze, SHEN Ying-fang. The Effects of MgSO₄ Stress on the Physiological Responses and Gene Expression of Alfalfa[J]. Acta Agrestia Sinica, 2025, 33(12): 3944-3957.

魏晓娜, 张湑泽, 沈迎芳. MgSO₄胁迫对紫花苜蓿生理响应及基因表达的影响[J]. 草地学报, 2025, 33(12): 3944-3957.

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The Effects of MgSO₄ Stress on the Physiological Responses and Gene Expression of Alfalfa

MgSO₄胁迫对紫花苜蓿生理响应及基因表达的影响

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[1]	SONG Chao-ran, JIA Yu-shan, WANG Zhi-jun, LIU Jian-liang, MA Wei-qin, SHENG Pan-jie, SI Qiang. Changes in Respiratory Metabolism and Nutritional Quality of Alfalfa during Drying in Saline-Alkali Land [J]. Acta Agrestia Sinica, 2025, 33(11): 3621-3632.
[2]	WEI Kong-qin, SUN Yan-liang, HUI Jin-feng, MA Chun-hui, ZHANG Qian-bing. Effects of Phosphate Solubilizing Bacteria on Photosynthetic Characteristics， Antioxidant Activity， and Yield of Alfalfa [J]. Acta Agrestia Sinica, 2025, 33(11): 3643-3653.
[3]	WEN Yan-bin, WANG Xin, WANG Na, SONG Hui-yu, DU Yan-gong. Effects of Alfalfa on Soil Fungal Community Formation in the Eastern Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2025, 33(10): 3225-3236.
[4]	WANG Yan, SHI Shang-li, YU Hai-tao, HAN Yi-lin. Evaluation on Salt Tolerance of 18 Alfalfa Cultivars During Germination [J]. Acta Agrestia Sinica, 2025, 33(10): 3341-3352.
[5]	WANG Xue-li, WANG Yun-ling, QI Kai-yuan, YAN Hai-jun, WANG Xian-guo, MENG Gen-qi-ge-mu, ZHOU Li-ye. Effects of Water Regulation on the Production Performance of Different Alfalfa （Medicago sativa L.） Varieties and Soil Enzymes [J]. Acta Agrestia Sinica, 2025, 33(9): 2808-2819.
[6]	SHI Jing, LIU Xiao-jing, LI Jia-qi, HAN Yan-long, WANG Xue, WANG Jing. Study on the Adaptability of 15 Alfalfa Varieties in Minqin Area [J]. Acta Agrestia Sinica, 2025, 33(9): 2843-2853.
[7]	YUAN Yu-tao, WU Li, WEI Xiao-xing, LIU Wen-hui, CHEN You-jun, HE Ke-yan, ZHOU Qing-ping. Production Performance and Quality Evaluation of 12 Alfalfa Varieties in the Semiarid Region of Hexi Corridor [J]. Acta Agrestia Sinica, 2025, 33(9): 3088-3098.
[8]	AN Wen, ZHANG Xin-yu, MA Dong-mei, MA Qiao-li, LANG Si-rui, LIU Xiao-xia. Cloning and Salt Tolerance Analysis of MsNF-YB8 Gene in Alfalfa [J]. Acta Agrestia Sinica, 2025, 33(8): 2456-2466.
[9]	JI Ling-he, HE Ao-lei, HE Feng, LI Chang-ning, YAO Tuo. Screening of Alfalfa Seed Coating Materials and Theirs Effect on Seed Germination [J]. Acta Agrestia Sinica, 2025, 33(8): 2728-2736.
[10]	HE Hai-feng, MA Jin-yan, XU Xing. Study on the Relationship between Photosynthetic Fluorescence Parameters and Quality of Alfalfa in Saline-Alkali Land [J]. Acta Agrestia Sinica, 2025, 33(7): 2123-2131.
[11]	HE Sheng-ran, LIU Xiao-jing, HAN Tian-hu, ZHAO Ya-jiao, HAN Yan-long. Effects of Alfalfa Nitrogen Efficiency Differences on Nutrient Characteristics and Microbial Community Characteristics of Rhizosphere Soil [J]. Acta Agrestia Sinica, 2025, 33(7): 2150-2161.
[12]	LU Hui, LI Qian, KAN Hai-ming, XU Heng-kang, PANG Zhuo, ZHANG Guo-fang, WANG Yu-xiang, CHEN Chao. Effects of Plant Hormones on the Hydrotropism and Gravitropism of Medicago falcata Root Tips [J]. Acta Agrestia Sinica, 2025, 33(6): 1826-1833.
[13]	LYU Yan-zhen, YAO Xing-jie, ZHANG Zhao, SUN Qing-ying, YANG Yu-ze, YAN Hui-fang. Evaluation of Alkali Tolerance and Key Indicators Screening during Seed Germination of Different Alfalfa Cultivars [J]. Acta Agrestia Sinica, 2025, 33(6): 1852-1861.
[14]	WANG Xin-yao, LIU Yi-xin, SUI Xiao-qing, LANG Meng-qing, JIN Lian-wu. Effects of Spray Mepiquat-Chloride on Leaf Cells， Agronomic Traits and Seed Yield Formation in Alfalfa [J]. Acta Agrestia Sinica, 2025, 33(6): 1972-1981.
[15]	WANG Zhao-ming, CHEN Xiang, ZHAO Jie, ZHAO Chen, QIU Wen-jing, FU Juan-juan, ZHANG Xiao-yan, XIE Hai-tao, WEI Rong-bo. Effects of Combined Application of Biochar and Nitrogen Fertilizer on the Agronomic Traits and Quality on Intercropping Alfalfa and Smooth Bromegrass Systems [J]. Acta Agrestia Sinica, 2025, 33(6): 1982-1990.