Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 323-330.DOI: 10.11733/j.issn.1007-0435.2026.01.030

Previous Articles    

Foliar Spraying of the New Osmolyte TMAO Improves the Drought Tolerance of Lotus japonicus

ZHENG Zhi-long1, HU Min1, NIU Kui-ju2, YANG Zhi-min1, ZHUANG Li-li1   

  1. 1. College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
    2. College of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China
  • Received:2025-06-16 Revised:2025-07-31 Published:2025-12-24

叶面喷施新型渗透剂TMAO提高百脉根耐旱性

郑智龙1, 胡敏1, 牛奎举2, 杨志民1, 庄黎丽1   

  1. 1. 南京农业大学草业学院, 江苏 南京 210095;
    2. 甘肃农业大学草业学院, 甘肃 兰州 730070
  • 通讯作者: 庄黎丽,E-mail:nauzll@njau.edu.cn
  • 作者简介:郑智龙（1999-），男，汉族，江西赣州人，硕士，主要从事豆科牧草生物技术研究，E-mail:zhilonzheng@163.com；
  • 基金资助:
    甘肃农业大学草业生态系统教育部重点实验室开放课题（编号：KLGE202211）；甘肃省自然科学基金项目（编号： 23JRRA1416）；国家自然科学基金面上项目（编号：32171689）资助

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Abstract: The objective of this study was to determine whether the novel osmolyte trimethylamine N-oxide （TMAO） enhances drought tolerance of Lotus japonicus and to preliminarily reveal the corresponding physiological mechanisms. L. japonicus ecotype ‘Miyakojima’ MG-20 was used in the study. Five concentrations of TMAO （0.1 mmol·L-1， 0.5 mmol·L-1， 1 mmol·L-1， 5 mmol·L-1， and 10 mmol·L-1） and ddH2O were sprayed on MG20 leaves daily for 7 d， plants were then immediately subjected to drought treatment for 28 d， followed by rewatering for 7 d. Based on plant phenotype， 5 mmol·L-1 TMAO was selected as the best concentration to improve the drought tolerance of L. japonicus. To further investigate the underlying physiological mechanism， 5 mmol·L-1 TMAO was sprayed on leaves for 7 d followed by drought tolerance evaluation. After 28 days of drought， plant height， leaf relative water content （LRWC）， chlorophyll content （Chl）， and photochemical efficiency （Fv/Fm） were significantly lower in both drought-stressed plants sprayed with ddH2O （D-W） or TMAO （D-T） compared to well-watered controls sprayed with ddH2O （W-W） or TMAO （W-T）. However， these parameters were significantly higher in the D-T group than in the D-W group. Concurrently， leaf relative electrolyte leakage （EL） and superoxide anion （O2-） accumulation in leaves were significantly higher in the D-W and D-T groups compared to the W-W and W-T groups， but significantly lower in the D-T group than in the D-W group. Following 7 days of rewatering， chlorophyll content remained significantly higher in the D-T group compared to the D-W group， while no significant differences were observed in the other measured parameters between these two groups. These results indicate that foliar application of TMAO can alleviate drought-induced damage in L. japonicus， providing an effective approach for improving cultivation under drought conditions.

Key words: Lotus japonicus, TMAO, Drought stress, Physiological index

摘要： 为确定新型渗透剂氧化三甲胺（Trimethylamine N-oxide，TMAO）是否能提高百脉根（Lotus japonicus）对干旱胁迫的耐受性，并初步揭示相应的生理机制，本研究以百脉根生态型‘Miyakojima’MG-20为供试材料，基于植株表型从不同浓度TMAO（ddH2O，0.1 mmol·L-1，0.5 mmol·L-1，1 mmol·L-1，5 mmol·L-1和10 mmol·L-1）中筛选出提高百脉根耐旱性的最佳浓度。以最佳浓度5 mmol·L-1的TMAO喷施百脉根，并进行干旱处理，结果表明：处理28天时，干旱喷施TMAO（D-T）的植株的株高、叶片相对含水量、叶绿素含量和光化学效率显著高于干旱喷施ddH2O（D-W）的植株，同时，D-T组的叶片电解质渗漏率和超氧阴离子积累显著低于D-W组的植株。复水7天后，D-T组叶绿素含量仍显著高于D-W组，但其余指标在两者间无显著差异。这些结果表明了叶面喷施TMAO可以缓解干旱胁迫对百脉根的伤害，为百脉根干旱栽培提供了有效的调控手段。

关键词: 百脉根, TMAO, 干旱胁迫, 生理指标

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