Effect of Silage Additives on Silage Quality of Different Varieties of Hybrid Pennisetum

doi:10.11733/j.issn.1007-0435.2026.03.034

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (3): 1118-1127.DOI: 10.11733/j.issn.1007-0435.2026.03.034

Effect of Silage Additives on Silage Quality of Different Varieties of Hybrid Pennisetum

TIAN Hao-yu¹, WANG Si-yi¹, YUAN Ming-wei¹, LIU Yi-ting¹, WANG Lian-jun², LIU Yu-fei³, GUI Jing³, YANG Xia³, QIAO Jiang⁴, ZHANG Yan-fen⁴, WU Zhe¹

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Yuyang District Forestry and Grassland Ecological Restoration Center, Yulin, Shaanxi Province 719000, China;
3. Yuyang District Grassland Work Station, Yulin, Shaanxi Province 719000, China;
4. Yulin Animal Husbandry and Veterinary Service Center, Yulin, Shaanxi Province 719000, China

Received:2025-03-13 Revised:2025-06-09 Published:2026-03-23

副干酪乳杆菌与枯草芽孢杆菌对不同品种杂交狼尾草青贮品质的影响

田浩宇¹, 王思怡¹, 苑明伟¹, 刘奕婷¹, 王连军², 刘宇飞³, 归静³, 杨霞³, 乔江⁴, 张艳芬⁴, 吴哲¹

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 榆阳区林草生态修复中心, 陕西榆林 719000;
3. 榆阳区草原工作站, 陕西榆林 719000;
4. 榆林市畜牧兽医服务中心, 陕西榆林 719000

通讯作者: 吴哲，E-mail：wuzhe@cau.edu.cn
作者简介:田浩宇（2002-），男，汉族，湖南邵阳人，硕士研究生，主要从事饲草加工利用研究，E-mail：2728433435@qq.com；
基金资助:
财政部和农业农村部：国家现代农业产业技术体系（CARS-34-24）；国家重点研发计划“政府间国际科技创新合作”重点专项（2022YFE0111000）；生态友好型苜蓿产品加工贮藏技术集成与应用（2022ZY0117）；榆林市秸秆与饲草综合利用技术研究与示范资助

Abstract

Abstract: This study was designed to systematically investigate the effects of different hybrid Pennisetum varieties and microbial additive treatments on the silage quality of hybrid Pennisetum. A total of six hybrid Pennisetum genotypes were employed， with each genotype assigned to a control group （CK）， Lactobacillus paracasei （LP） group， Bacillus subtilis （BS） group， and Lactobacillus paracasei + Bacillus subtilis （LP+BS） group. The inoculation dosages for both L. paracasei and B. subtilis were standardized at 1×10⁶ CFU·g^-1 fresh weight （FW）. Following a 30-day fermentation period， nutritional parameters and fermentation indices were quantified， with comprehensive evaluation conducted via Flieg scoring system and principal component analysis （PCA）.Results demonstrated that probiotic amendments significantly enhanced crude protein （CP） content in‘PI_587000’and‘536321’genotypes， while markedly reducing neutral detergent fiber （NDF） content in ‘536321’and‘103’genotypes. Supplementation with LP and LP+BS notably decreased the ammonia nitrogen ∶ total nitrogen （NH₃-N∶TN） ratio in‘Sunongke No.2’‘ 536321’‘102’and‘103’ （P<0.05）， whereas all BS-treated groups exhibited significantly higher NH₃-N∶TN ratios than LP+BS treatments （P<0.05）. Genotypes‘102’and‘103’showed elevated lactic acid （LA） accumulation. Integrative analysis of Flieg scores and NH₃-N∶TN ratios indicated that ‘late-maturing Pennisetum’displayed optimal fermentation performance， with LP+BS demonstrating the most pronounced improvement in forage silage quality. PCA yielded five principal components with a cumulative contribution rate of 86.558%， confirming‘late-maturing Pennisetum’as the top-performing genotypes and LP+BS as the most effective additive treatment. Conclusively，‘late-maturing Pennisetum’is preferable as silage substrate for hybrid Pennisetum， and the LP+BS consortium represents the optimal probiotic additive for enhancing silage quality.

Key words: Pennisetum americanum×P.purpureum, silage quality, Lactobacillus paracasei, Bacillus subtilis

摘要： 本试验旨在研究不同杂交狼尾草品种及添加剂处理对杂交狼尾草青贮品质的影响。试验共6种杂交狼尾草，每个品种设置对照组（CK）、副干酪乳杆菌组（LP）、枯草芽孢杆菌组（BS）及副干酪乳杆菌+枯草芽孢杆菌组合接种组（LP+BS），副干酪乳杆菌、枯草芽孢杆菌的添加量均为1×10⁶ CFU·g^-1 FW，发酵30 d后测定营养与发酵指标，并通过弗氏评分和主成分分析综合评价。结果显示：添加剂处理显著提高‘PI_587000’‘536321’两个品种的粗蛋白含量并显著降低‘536321’‘103’两个品种的中性洗涤纤维含量；LP和LP+BS能显著降低‘苏农科二号’‘536321’‘102’和‘103’的NH₃?N∶TN（P<0.05），但所有品种BS处理的氨态氮∶全氮显著高于LP+BS处理（P<0.05）；‘102’和‘103’两个品种的乳酸含量较高。弗氏评分结合氨态氮∶全氮分析显示，‘晚熟狼尾草’发酵效果最好；LP+BS作为青贮添加剂对饲草青贮改善效果最好。主成分分析提取5个主成分，累积贡献率达到86.558%。各品种综合得分最高的是‘晚熟狼尾草’品种，各添加剂处理综合得分最高的是LP+BS。综上，‘晚熟狼尾草’更适合作为杂交狼尾草青贮原料，添加剂LP+BS更适合作为杂交狼尾草的青贮添加剂。

关键词: 杂交狼尾草, 青贮品质, 副干酪乳杆菌, 枯草芽孢杆菌

CLC Number:

S816.53

TIAN Hao-yu, WANG Si-yi, YUAN Ming-wei, LIU Yi-ting, WANG Lian-jun, LIU Yu-fei, GUI Jing, YANG Xia, QIAO Jiang, ZHANG Yan-fen, WU Zhe. Effect of Silage Additives on Silage Quality of Different Varieties of Hybrid Pennisetum[J]. Acta Agrestia Sinica, 2026, 34(3): 1118-1127.

田浩宇, 王思怡, 苑明伟, 刘奕婷, 王连军, 刘宇飞, 归静, 杨霞, 乔江, 张艳芬, 吴哲. 副干酪乳杆菌与枯草芽孢杆菌对不同品种杂交狼尾草青贮品质的影响[J]. 草地学报, 2026, 34(3): 1118-1127.

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Effect of Silage Additives on Silage Quality of Different Varieties of Hybrid Pennisetum

副干酪乳杆菌与枯草芽孢杆菌对不同品种杂交狼尾草青贮品质的影响

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