Study on the Variation Characteristics of Glomalin-Related Soil Protein and Soil Organic Carbon at Different Succession Stages of Alpine Meadow

doi:10.11733/j.issn.1007-0435.2026.05.020

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (5): 1787-1797.DOI: 10.11733/j.issn.1007-0435.2026.05.020

Study on the Variation Characteristics of Glomalin-Related Soil Protein and Soil Organic Carbon at Different Succession Stages of Alpine Meadow

YANG Xiao-xuan¹, LI Run-jie², CUI Zi-long^2,3, XIN Ji-lin^1,2, LIN Cheng-qing^1,2, KONG Xiao-yun^1,2, DANG Yi-le^1,2, MA Zhan-ming^1,2, ZHANG Ming-yang^4,5, ZHANG Yong-kun²

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, China;
3. College of Ecological and Environmental Engineering, Qinghai University, Xining, Qinghai Province 810016, China;
4. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810016, China;
5. Key Laboratory of Plateau Adaptation and Evolution, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining Qinghai Province 810008, China;
6. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2025-01-02 Revised:2025-07-31 Published:2026-05-08

高寒草甸不同演替阶段球囊霉素相关土壤蛋白与有机碳变化特征研究

杨晓璇¹, 李润杰², 崔子龙^2,3, 辛继林^1,2, 林成清^1,2, 孔小云^1,2, 党怡乐^1,2, 马占明^1,2, 张铭洋^4,5, 张永坤²

1. 青海大学农牧学院, 青海西宁 810016;
2. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海西宁 810016;
3. 青海大学生态环境工程学院, 青海西宁 810016;
4. 中国科学院西北高原生物研究所高原生物适应与进化重点实验室, 青海西宁 810008;
5. 中国科学院大学, 北京 100049;
6. null

通讯作者: 张永坤，E-mail:zhangyongkun321@163.com
作者简介:杨晓璇（1999-），女，汉族，青海海东人，硕士研究生，主要从事土壤生态学研究，E-mail:24983391802@qq.com；
基金资助:
国家自然科学基金（4220072854）；青海省应用基础研究项目（2022-ZJ-716）共同资助

Abstract

Abstract: To elucidate the variation patterns of glomalin-related soil protein （GRSP） and their underlying drivers during the degradation and restoration of alpine meadows， this study quantified key soil physicochemical properties， soil organic carbon （SOC）， easily extractable GRSP （E-GRSP）， and total GRSP （T-GRSP） in both the 0-10 cm and 10-20 cm soil layers. The relationships between these variables and potential driving factors were analyzed using Pearson correlation analysis and redundancy analysis. Results showed that in the 0-10 cm layer， SOC decreased significantly with grassland degradation （P<0.05）， and artificial restoration was more effective than natural regeneration in enhancing SOC levels. Both E-GRSP and T-GRSP in the 0-10 cm layer exhibited an initial decline followed by a subsequent increase during the processes of degradation and recovery （P<0.05）， whereas the ratios of E-GRSP/SOC and T-GRSP/SOC displayed an opposite trend. Pearson correlation analysis revealed a highly significant positive correlation （P<0.01） between T-GRSP， E-GRSP， and SOC across the 0-20 cm soil profile. Redundancy analysis indicated that SOC was the primary explanatory variable for variations in both GRSP content and GRSP/SOC ratios throughout the 0-20 cm soil depth. Collectively， these findings suggest that grassland restoration promotes the preservation of GRSP in alpine meadow soils， and increased GRSP content contributes to enhanced SOC accumulation， highlighting its crucial role in soil carbon sequestration and the stability of soil carbon pools. This study provides valuable insights into carbon sequestration mechanisms in grassland ecosystems， supports improvements in soil structure stability， facilitates soil quality assessment， and offers practical guidance for ecological restoration efforts.

Key words: Glomalin-related soil protein, Soil organic carbon, Qinghai-Tibet Plateau, Alpine grassland, Grassland restoration

摘要： 为揭示高寒草甸退化与恢复过程中球囊霉素相关土壤蛋白（Glomalin-related soil protein， GRSP）变化特征及其影响因素，本研究通过测定0~10 cm与10~20 cm土层土壤理化性质、土壤有机碳（Soil organic carbon， SOC）、土壤易提取球囊霉素相关土壤蛋白（E-GRSP）和总球囊霉素相关土壤蛋白（T-GRSP）的含量，并借助皮尔逊相关分析（Pearson）与冗余分析探讨其与驱动因子的关系。结果表明：在0~10 cm土层，SOC随草地退化显著降低（P<0.05），且人工修复方式对退化草地SOC的提升效果优于自然恢复方式。在0~10 cm土层E-GRSP和T-GRSP在退化与恢复过程中表现出先降低后升高的趋势（P<0.05），而E-GRSP/SOC和T-GRSP/SOC的变化规律与之相反。Pearson相关分析表明，在0~20 cm土层T-GRSP、E-GRSP与SOC呈极显著正相关（P<0.01）；冗余分析发现，在0~20 cm土层，SOC是对GRSP含量、GRSP/SOC比值解释度最高的因子。综合分析显示，草地恢复对高寒草甸土壤中球囊霉素相关土壤蛋白（GRSP）的维持具有促进作用，而GRSP含量升高促进SOC积累，表明其在土壤碳固持及碳库维系中起关键作用。本研究对揭示草地生态系统碳固持机制、提升土壤结构稳定性、评估土壤质量及指导草地生态修复具有重要意义。

CLC Number:

S812.2

YANG Xiao-xuan, LI Run-jie, CUI Zi-long, XIN Ji-lin, LIN Cheng-qing, KONG Xiao-yun, DANG Yi-le, MA Zhan-ming, ZHANG Ming-yang, ZHANG Yong-kun. Study on the Variation Characteristics of Glomalin-Related Soil Protein and Soil Organic Carbon at Different Succession Stages of Alpine Meadow[J]. Acta Agrestia Sinica, 2026, 34(5): 1787-1797.

杨晓璇, 李润杰, 崔子龙, 辛继林, 林成清, 孔小云, 党怡乐, 马占明, 张铭洋, 张永坤. 高寒草甸不同演替阶段球囊霉素相关土壤蛋白与有机碳变化特征研究[J]. 草地学报, 2026, 34(5): 1787-1797.

References

[1] BAO Z P, LIN D, LIU X P, et al. Effects of grazing intensity on soil organic carbon and its components in alpine meadow[J]. Acta Agrestia Sinica, 2026, 34(1): 172-181 包志鹏, 林栋, 刘雪鹏, 等.放牧强度对高寒草甸土壤有机碳及其组分的影响[J].草地学报, 2026, 34(1):172-181
[2] MA Z L, ZHAO W Q. Research Progress on Plant Community Input to Soil Organic Carbon and Its Response to Climate Warming[J]. Chinese Journal of Ecology, 2020, 39(1):270-281 马志良, 赵文强. 植物群落向土壤有机碳输入及其对气候变暖的响应研究进展[J]. 生态学杂志, 2020, 39(1):270-281
[3] MI Y Q, XU H B, ZHANG L, et al. Effects of enclosure on vegetation niche and interspecific association in desert steppe of Inner Mongolia[J]. Acta Agrestia Sinica, 2026, 34(3): 1007-1016 米雨晴, 许宏斌, 张雷, 等.围封对内蒙古荒漠草原植被生态位及种间联结性的影响[J].草地学报, 2026, 34(03):1007-1016
[4] SONG J, WAN S, PENG S S, et al. The carbon sequestration potential of China’s grasslands. Ecosphere, 2018, 9(10):e02452
[5] LIU Y S, FAN J W, SHI Z J, et al. Relationships between plateau pika (Ochotona curzoniae) densities and biomass and biodiversity indices of alpine meadow steppe on the Qinghai-Tibet Plateau China[J]. Ecological Engineering, 2017, 102:509-518
[6] SONG X A, LI S X, ZHAO W, et al. Effects of different restoration methods on vegetation and soil fungal community characteristics in degraded alpine grassland[J]. Acta Agrestia Sinica, 2025, 33(12): 3958-3968 宋学澳, 李世雄, 赵文, 等.不同修复方式对退化高寒草地植被及土壤真菌群落特征的影响[J].草地学报, 2025, 33(12):3958-3968
[7] BARBOSA M V, DE FÁTIMA PEDROSO D, CURI N, et al. Do different arbuscular mycorrhizal fungi affect the formation and stability of soil aggregates？[J]. Ciência e Agrotecnologia, 2019, 43:e003519
[8] SINGH A K, ZHU X A, CHEN C F, et al. The role of glomalin in mitigation of multiple soil degradation problems[J]. Critical Reviews in Environmental Science and Technology, 2022, 52(9):1604-1638
[9] RILLIG M C, WRIGHT S F, NICHOLS K A, et al. Large contribution of arbuscular mycorrhizal fungi to soil carbon pools in tropical forest soils[J]. Plant and Soil, 2001, 233(2):167-177
[10] GISPERT M, PARDINI G, EMRAN M, et al. Seasonal evolution of soil organic matter, glomalin and enzymes and potential for C storage after land abandonment and renaturalization processes in soils of NE Spain[J]. Catena, 2018, 162:402-413
[11] KUMAR S, SINGH A K, GHOSH P. Distribution of soil organic carbon and glomalin related soil protein in reclaimed coal mine-land chronosequence under tropical condition[J]. Science of the Total Environment, 2018, 625:1341-1350
[12] WANG W J, ZHONG Z L, WANG Q, et al. Glomalin contributed more to carbon, nutrients in deeper soils, and differently associated with climates and soil properties in vertical profiles[J]. Scientific Reports, 2017, 7:13003
[13] SINGH A K, RAI A, PANDEY V, et al. Contribution of glomalin to dissolve organic carbon under different land uses and seasonality in dry tropics[J]. Journal of Environmental Management, 2017, 192:142-149
[14] WANG X L, CAO Q J, YANG W Y, et al. Spatial changes in glomalin-related soil protein and their correlation with soil properties in the black soil region of Northeast China[J]. Agronomy, 2022, 12(9):2165
[15] BANEGAS N, DOS SANTOS D A, GUERRERO MOLINA F, et al. Glomalin contribution to soil organic carbon under different pasture managements in a saline soil environment[J]. Archives of Agronomy and Soil Science, 2022, 68(3):340-354
[16] XU M, LI X L, CAI X B, et al. Land use alters arbuscular mycorrhizal fungal communities and their potential role in carbon sequestration on the Tibetan Plateau[J]. Scientific Reports, 2017, 7:3067
[17] LIU G C, DUAN X L, YAN G Y, et al. Changes in soil aggregates and glomalin-related soil protein stability during the successional process of boreal forests[J]. Journal of Soil Science and Plant Nutrition, 2024, 24(1):1335-1348
[18] QIAO L L, LI Y Z, SONG Y H, et al. Effects of vegetation restoration on the distribution of nutrients, glomalin-related soil protein, and enzyme activity in soil aggregates on the Loess Plateau, China[J]. Forests, 2019, 10(9):796
[19] HOU H, YAN P X, XIE Q M, et al. Distribution characteristics and influence factors of rhizosphere glomalin-related soil protein in three vegetation types of Helan Mountain, China[J]. Forests, 2022, 13(12):2092
[20] MA X D, LI J P, DING F C, et al. Changes of arbuscular mycorrhizal fungal community and glomalin in the rhizosphere along the distribution gradient of zonal Stipa populations across the arid and semiarid steppe[J]. Microbiology Spectrum, 2022, 10(5):e01489-22
[21] WANG Q, WANG W J, ZHONG Z L, et al. Variation in glomalin in soil profiles and its association with climatic conditions, shelterbelt characteristics, and soil properties in poplar shelterbelts of Northeast China[J]. Journal of Forestry Research, 2020, 31(1):279-290
[22] CISSÉ G, ESSI M, KEDI B, et al. Accumulation and vertical distribution of glomalin-related soil protein in French temperate forest soils as a function of tree type, climate and soil properties[J]. Catena, 2023, 220:106635
[23] ZHANG J, TANG X L, HE X H, et al. Glomalin-related soil protein responses to elevated CO₂ and nitrogen addition in a subtropical forest: potential consequences for soil carbon accumulation[J]. Soil Biology and Biochemistry, 2015, 83:142-149
[24] LI T T, YUAN Y, MOU Z J, et al. Faster accumulation and greater contribution of glomalin to the soil organic carbon pool than amino sugars do under tropical coastal forest restoration[J]. Global Change Biology, 2023, 29(2):533-546
[25] GU R, XIAO K C, ZHU Z H, et al. Afforestation enhances glomalin-related soil protein content but decreases its contribution to soil organic carbon in a subtropical karst area[J]. Journal of Environmental Management, 2024, 356:120754
[26] WANG Q, LU H L, CHEN J Y, et al. Spatial distribution of glomalin-related soil protein and its relationship with sediment carbon sequestration across a mangrove forest[J]. Science of the Total Environment, 2018, 613/614:548-556
[27] PEI L X, YE S Y, YUAN H M, et al. Glomalin-related soil protein distributions in the wetlands of the Liaohe Delta, Northeast China: Implications for carbon sequestration and mineral weathering of coastal wetlands[J]. Limnology and Oceanography, 2020, 65(5): 979-991
[28] ZHANG Y J, HE X L, ZHAO L L, et al. Dynamics of arbuscular mycorrhizal fungi and glomalin under Psammochloa villosa along a typical dune in desert, North China[J]. Symbiosis, 2017, 73(3):145-153
[29] HE X L, LI Y P, ZHAO L L. Dynamics of arbuscular mycorrhizal fungi and glomalin in the rhizosphere of Artemisia ordosica Krasch. in Mu Us sandland, China[J]. Soil Biology and Biochemistry, 2010, 42(8):1313-1319
[30] CHEN H L, MA C, YAN L D. Analysis of Climate Change characteristics and Its Impact on Natural Grassland Forage Yield in Henan County, Qinghai Province (1981-2022)[J]. Qinghai Prataculture, 2023, 32(3): 71-75 陈海莲, 马超, 颜亮东. 1981—2022年青海省河南县气候变化及其对天然草地产草量的影响分析[J]. 青海草业, 2023, 32(3):71-75
[31] LI G R, LI X L, LI J F, et al. Soil Wind Erosion Patterns of Plateau Pika Mounds in Alpine Meadows at the Source of the Yellow River[J]. Journal of Soil and Water Conservation, 2019, 33(2): 110-114, 168 李国荣, 李希来, 李进芳, 等. 黄河源高寒草甸高原鼠兔土丘的土壤风力侵蚀规律[J]. 水土保持学报, 2019, 33(2):110-114, 168
[32] BAO S D. Soil and Agricultural Chemical Analysis[M]. 3rd ed. Beijing: China Agriculture Press, 2000: 15-20 鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社, 2000:15-20
[33] WRIGHT S F, UPADHYAYA A. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi[J]. Plant and Soil, 1998, 198(1):97-107
[34] LI Y J, SUN C C, CAO G G, et al. Characteristics of Biomass and Soil Nutrients in Grasslands under Different Utilization Patterns in the Three-River Headwaters Region[J]. Grassland and Turf, 2016, 36(4): 48-53 李亚娟, 孙灿灿, 曹广民, 等. 三江源区不同利用方式草地生物量及土壤养分特征[J]. 草原与草坪, 2016, 36(4):48-53
[35] YAN Y C, TANG H P. Restoration of Degraded Grassland Communities and Its Contribution to Carbon Sequestration under Enclosure in a Typical Steppe Region of Inner Mongolia[J]. Progress in Natural Science, 2008, 18(5): 546-551 闫玉春, 唐海萍.围封下内蒙古典型草原区退化草原群落的恢复及其对碳截存的贡献.自然科学进展, 2008, 18(5):546-551
[36] SINGH P K, SINGH M, TRIPATHI B N. Glomalin: an arbuscular mycorrhizal fungal soil protein[J]. Protoplasma, 2013, 250(3):663-669
[37] YANG M, SHI Z Y, LU SCH, et al. Effects of Warming on Soil Glomalin Content in Grassland Ecosystems of the Qinghai-Tibet Plateau[J]. Ecology and Environmental Sciences, 2020, 29(4):650-656 杨梅, 石兆勇, 卢世川, 等. 增温对青藏高原草地生态系统土壤球囊霉素含量的影响[J]. 生态环境学报, 2020, 29(4):650-656
[38] QIAO L L, LI Y Z, SONG Y H, et al. Effects of vegetation restoration on the distribution of nutrients, glomalin-related soil protein, and enzyme activity in soil aggregates on the Loess Plateau, China[J]. Forests, 2019, 10(9):796
[39] Li B W. Effects of Fertilization on Glomalin-Related Soil Protein and Environmental Factors in Alpine Meadows of the Qinghai-Tibet Plateau[D]. Lanzhou: Lanzhou University, 2016:27-29 李博文. 施肥对青藏高原高寒草甸球囊霉素土壤相关蛋白及其环境因子的影响[D]. 兰州:兰州大学, 2016:27-29
[40] WU J P, LIU W F, FAN H B, et al. Asynchronous responses of soil microbial community and understory plant community to simulated nitrogen deposition in a subtropical forest[J]. Ecology and Evolution, 2013, 3(11):3895-3905
[41] GOSLING P, MEAD A, PROCTOR M, et al. Contrasting arbuscular mycorrhizal communities colonizing different host plants show a similar response to a soil phosphorus concentration gradient[J]. New Phytologist, 2013, 198(2):546-556
[42] WANG Q, WU Y, WANG W J, et al. Spatial variations in concentration, compositions of glomalin related soil protein in poplar plantations in northeastern China, and possible relations with soil physicochemical properties[J]. The Scientific World Journal, 2014, 2014(1):160403
[43] MA X D, LI J P, DING F C, et al. Changes of arbuscular mycorrhizal fungal community and glomalin in the rhizosphere along the distribution gradient of zonal Stipa populations across the arid and semiarid steppe[J]. Microbiology Spectrum, 2022, 10(5)
[44] AGNIHOTRI R, SHARMA M P, PRAKASH A, et al. Glycoproteins of arbuscular mycorrhiza for soil carbon sequestration: review of mechanisms and controls[J]. Science of the Total Environment, 2022, 806:150571
[45] HE X L, CHEN C, HE B. Spatial distribution of arbuscular mycorrhizal fungi and glomalin of Hippophae rhamnoides L in farming-pastoral zone from the two northern provinces of China[J]. Acta Ecologica Sinica, 2011, 31(6)
[46] VODNIK D, GRČMAN H, MAČEK I, et al. The contribution of glomalin-related soil protein to Pb and Zn sequestration in polluted soil[J]. Science of the Total Environment, 2008, 392(1):130-136
[47] BANEGAS N, DOS SANTOS D A, GUERRERO MOLINA F, et al. Glomalin contribution to soil organic carbon under different pasture managements in a saline soil environment[J]. Archives of Agronomy and Soil Science, 2022, 68(3):340-354

Study on the Variation Characteristics of Glomalin-Related Soil Protein and Soil Organic Carbon at Different Succession Stages of Alpine Meadow

高寒草甸不同演替阶段球囊霉素相关土壤蛋白与有机碳变化特征研究

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