Proteomic Profiling of Exogenous Brassinolide-Induced Resistance to Leaf Spot Disease in Oat

doi:10.11733/j.issn.1007-0435.2026.10.009

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (10): 3616-3627.DOI: 10.11733/j.issn.1007-0435.2026.10.009

Proteomic Profiling of Exogenous Brassinolide-Induced Resistance to Leaf Spot Disease in Oat

CHEN Yue-jin, ZHAO Gui-qin, NIU Kui-ju, WANG Zheng

College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province/Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2025-12-05 Revised:2026-02-19 Published:2026-10-10

外源油菜素内酯诱导燕麦叶斑病抗性的蛋白质组学研究

陈月进, 赵桂琴, 牛奎举, 王正

甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 赵桂琴, E-mail: zhaogq@gsau.edu.cn
作者简介:陈月进（2002-），女，汉族，甘肃平凉人，硕士研究生，主要从事燕麦抗病生理方向的研究，E-mail：3334773376@qq.com
基金资助:
国家自然基金（32360342）；国家燕麦荞麦产业技术体系（CARS-7-C1）资助

Abstract

Abstract: 2，4-Epibrassinolide （EBR） can enhance the immunity of plants against various diseases. This study， based on proteomics， reveals the molecular basis of exogenous EBR-induced resistance to Drechslera avenae leaf spot disease in oats （Avena sativa）. Four treatments were set up： control， oat D. avenae inoculation group， exogenous EBR group， and exogenous EBR+oat D. avenae inoculation group. The oat leaves were subjected to proteomics analysis 10 days after treatment. Combined with GO/KEGG functional annotation and protein interaction network， a total of 599 differentially expressed proteins （DEPs） were identified. These DEPs were mainly enriched in carbohydrate metabolism， glutathione metabolism， porphyrin metabolism， and cellular protein metabolism pathways， while inducing defense responses such as lignin biosynthesis and redox. Exogenous EBR treatment could enhance the photosynthesis-related pathways and maintain cellular stability through nuclear remodeling and cytoplasmic membrane function； alanine-glyoxylate aminotransferase agt1， photosystem II protein psbF， phosphoglucose isomerase PgiC， ATP synthase subunit atpA/atpB， and key enzyme CAD5 in phenylpropanoid synthesis were significantly upregulated， hub proteins such as NR1， agt1， and NYC1 might play a core role in EBR-induced immunity to oat leaf spot disease.

Key words: 2，4-Epibrassinolide, Avena sativa, Leaf spot, Disease resistance induction, Proteomic profiling

摘要： 2，4-表油菜素内酯（2，4-Epibrassinolide，EBR）可增强植物对多种病害的免疫力。本研究基于蛋白质组学，揭示外源EBR诱导燕麦（Avena sativa）抗德氏霉（Drechslera avenae）叶斑病的分子基础。设四组处理：对照、燕麦德氏霉接种组、外源EBR组、外源EBR+燕麦德氏霉接种组，对处理10 d后的叶片进行蛋白质组学分析、功能注释及蛋白互作网络分析，共鉴定到599个差异表达蛋白（DEPs），这些DEPs主要富集在碳水化合物代谢、谷胱甘肽代谢、卟啉代谢及细胞蛋白质代谢通路，同时诱导木质素生物合成和氧化还原等防御反应。外源EBR处理可增强光合作用相关通路，并通过核小体重构、细胞质稳态调节及质膜功能协同维持细胞微环境稳定；丙氨酸乙醛酸转氨酶agt1、光系统II蛋白psbF、磷酸葡萄糖异构酶PgiC及ATP合成酶亚基atpA/atpB，以及苯丙素合成关键酶CAD5显著上调；NR1，agt1及NYC1等枢纽蛋白可能在EBR诱导的燕麦叶斑病免疫反应中发挥着核心调控功能。

关键词: 2，4-表油菜素内酯, 燕麦, 叶斑病, 诱导抗病, 蛋白质组学分析

CLC Number:

S435.4

CHEN Yue-jin, ZHAO Gui-qin, NIU Kui-ju, WANG Zheng. Proteomic Profiling of Exogenous Brassinolide-Induced Resistance to Leaf Spot Disease in Oat[J]. Acta Agrestia Sinica, 2026, 34(10): 3616-3627.

陈月进, 赵桂琴, 牛奎举, 王正. 外源油菜素内酯诱导燕麦叶斑病抗性的蛋白质组学研究[J]. 草地学报, 2026, 34(10): 3616-3627.

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Proteomic Profiling of Exogenous Brassinolide-Induced Resistance to Leaf Spot Disease in Oat

外源油菜素内酯诱导燕麦叶斑病抗性的蛋白质组学研究

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