Optimal of ISSR Marker in Dactylis glomerata L.

doi:10.11733/j.issn.1007-0435.2007.03.018

›› 2007, Vol. 15 ›› Issue (3): 290-292,295.DOI: 10.11733/j.issn.1007-0435.2007.03.018

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Optimal of ISSR Marker in Dactylis glomerata L.

ZENG Bing^1,2, ZHANG Xin-quan¹, LAN Ying²

1. Sichuan Agricultural University, Sichuan Yaan, 625014;
2. Southwest University, Chongqing Rongchang 402460

Received:2006-05-29 Revised:2007-03-20 Online:2007-06-15 Published:2007-06-15

鸭茅ISSR反应体系的建立与优化(简报)

曾兵^1,2, 张新全¹, 兰英²

1. 四川农业大学, 四川, 雅安, 625014;
2. 西南大学, 重庆, 荣昌, 402460

作者简介:曾兵(1978- ),男,四川乐至人,博士研究生,研究方向为牧草及草坪草育种与种质资源,E-mail:zbin78@163.com
基金资助:
国家自然科学基金项目(30371022);教育部“新世纪优秀人才支持计划”(NCET-04-0909)资助

Abstract

Key words: Dactylis glomerata L., ISSR Marker, Reaction system, Optimizing

摘要： 简单序列重复区间扩增多态性(Inter-simple sequence repeat,简称ISSR)是由zietkiewicz等于1994年创建的一种DNA标记技术^[1]。ISSR技术的原理和操作与SSR、RAPD非常相似,只是引物设计要求不同,但其产物多态性比RFLP、SSR、RAPD丰富,可提供更多的基因组信息,比RAPD技术更加稳定可靠,实验重复性好^[2]。

关键词: 鸭茅, ISSR分子标记, 反应体系, 优化

CLC Number:

Q943

ZENG Bing, ZHANG Xin-quan, LAN Ying. Optimal of ISSR Marker in Dactylis glomerata L.[J]. , 2007, 15(3): 290-292,295.

曾兵, 张新全, 兰英. 鸭茅ISSR反应体系的建立与优化(简报)[J]. , 2007, 15(3): 290-292,295.

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Optimal of ISSR Marker in Dactylis glomerata L.

鸭茅ISSR反应体系的建立与优化(简报)

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