AFLP Analysis on Interspecific Hybrid F1 and the Parents between Elymus canadensis L.and E.dahuricus Turcz.or E.cylindricus（Franch.） Honda

doi:10.11733/j.issn.1007-0435.2008.05.001

›› 2008, Vol. 16 ›› Issue (5): 431-435,441.DOI: 10.11733/j.issn.1007-0435.2008.05.001

AFLP Analysis on Interspecific Hybrid F₁ and the Parents between Elymus canadensis L.and E.dahuricus Turcz.or E.cylindricus（Franch.） Honda

YU Zhuo, MA Yan-hong, LI Xiao-lei, ZHOU Ya-xing, LI Xiao-yu, LI Zao-zhe

College of Agronomy, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010019, China

Received:2008-04-11 Revised:2008-07-21 Online:2008-10-15 Published:2008-10-15

加拿大披碱草与披碱草、圆柱披碱草2个种间杂种F₁及其亲本的AFLP分析

于卓, 马艳红, 李小雷, 周亚星, 李晓宇, 李造哲

内蒙古农业大学农学院, 呼和浩特, 010019

作者简介:于卓(1958- ),男,博士,教授,博士生导师,从事饲用作物种质资源与遗传育种研究,E-mail:yuzhu058@sina.com
基金资助:
国家重点基础研究发展计划(973计划)项目(2007CB108901);国家自然科学基金项目(30560100);内蒙古自然科学基金项目(200607010505)

Abstract

Abstract: In order to provide a molecular basis for identification of hybrids and breeding of progenies,the genetic difference of Elymus canadensis L.×E.dahuricus Turcz.hybrid F₁,E.canadensis×E.cylindricus(Franch.) Honda hybrid F₁ and their parents were compared and analyzed by AFLP molecular marker technique.The results show that 1247 loci of AFLP markers were amplified with 13 suitable primer combinations,and 1042 loci among which were polymorphic and the percentage of polymorphism loci reached to 83.56%.The genetic distance of test plants was ranged from 0.4276 to 0.7486.Taking the genetic distance value of 0.65 as threshold,5 test plants were clustered into 2 groups:the first group included E.canadensis,E.cylindricus,and E.canadensis×E.cylindricus hybrid F₁;the second groups included E.dahuricus and E.canadensis×E.dahuricus hybrid F₁.

Key words: Elymus canadensis L., E.dahuricus Turcz., E.cylindricus(Franch.) Honda, Interspecific hybrid F₁, AFLP analysis

摘要： 以加拿大披碱草(Elymus canadensis L.)与披碱草(E.dahuricus Turcz.)、圆柱披碱草(E.cylindricus(Franch.)Honda)2个种间杂种F₁及其亲本为试材,利用AFLP分子标记技术,对2个杂种F₁及其亲本的遗传差异性进行比较分析,以期为杂种鉴定及后代选育提供分子依据。结果表明:用筛选出的13个适宜引物共扩增出1247个AFLP位点,其中多态性位点1042个,多态性位点比率高达83.56%;各供试材料的遗传距离(GD)变动在0.4276~0.7486之间,以GD值0.65为基准,5个材料聚为2类,第1类为加拿大披碱草、圆柱披碱草和加拿大披碱草×圆柱披碱草杂种F₁,第2类为披碱草、加拿大披碱草×披碱草杂种F₁。

关键词: 加拿大披碱草, 披碱草, 圆柱披碱草, 种间杂种F₁, AFLP分析

CLC Number:

Q943

YU Zhuo, MA Yan-hong, LI Xiao-lei, ZHOU Ya-xing, LI Xiao-yu, LI Zao-zhe. AFLP Analysis on Interspecific Hybrid F₁ and the Parents between Elymus canadensis L.and E.dahuricus Turcz.or E.cylindricus（Franch.） Honda[J]. , 2008, 16(5): 431-435,441.

于卓, 马艳红, 李小雷, 周亚星, 李晓宇, 李造哲. 加拿大披碱草与披碱草、圆柱披碱草2个种间杂种F₁及其亲本的AFLP分析[J]. , 2008, 16(5): 431-435,441.

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AFLP Analysis on Interspecific Hybrid F₁ and the Parents between Elymus canadensis L.and E.dahuricus Turcz.or E.cylindricus（Franch.） Honda

加拿大披碱草与披碱草、圆柱披碱草2个种间杂种F₁及其亲本的AFLP分析

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