Effect of Adding Cornzyme on Fermentation Quality and Carbohydrate Contents of Napiergrass Silages

doi:10.11733/j.issn.1007-0435.2009.01.023

›› 2009, Vol. 17 ›› Issue (1): 121-124.DOI: 10.11733/j.issn.1007-0435.2009.01.023

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Effect of Adding Cornzyme on Fermentation Quality and Carbohydrate Contents of Napiergrass Silages

JIA Yan-xia¹, YU Zhu², SHAO Tao¹

1. College of Animal Science and Technology, Nanjing Agriculture University, Nanjing, Jiangsu Province 210095, China;
2. Institute of Grassland Sciences, China Agricultural University, Beijing 100193, China

Received:2008-04-03 Revised:2008-05-26 Online:2009-02-15 Published:2009-02-15

添加酶制剂对象草青贮发酵品质的影响

贾燕霞¹, 玉柱², 邵涛¹

1. 南京农业大学动物科学技术学院, 江苏, 南京, 210095;
2. 中国农业大学草地研究所, 北京, 100193

通讯作者: 邵涛,Author for correspondence,E-mail:taoshaolan@yahoo.com.cn
作者简介:贾燕霞(1979- ),女,蒙古族,内蒙古乌盟人,硕士研究生,主要研究方向为反刍动物营养与饲料科学
基金资助:
国家自然基金重点项目(30730069);国家自然基金面上项目(30600426)

Abstract

Abstract: The aim of the experiment was to evaluate the effects of adding cornzyme on the fermentation qualities and carbohydrate contents of napiergrass silages.There was the control and four treatments of different cornzyme addition ratios as follows:(1) E1:cornzyme addition at 0.5 mL·kg-1,(2) E2:at 1.0 mL·kg^-1,(3) E3:at 1.5 mL·kg^-1,(4) E4:at 2.0 mL·kg^-1 based on fresh weight of napiergrass.The results showed that all treatments had good fermentation quality with low pH value(4.28-3.76) and NH3-N/total N ratio,none or only small amounts of propionic acid and butyric acid,high ratio of lactic acid/acetic acid(2.94-13.46),and lactic acid content(45.00-95.41 g·kg^-1 DM).Cornzyme addition significantly increased the ratio of lactic acid/acetic acid and the contents of lactic acid and water soluble carbohydrate(P<0.05);and significantly decreased the pH values and the contents of acetic acid and total volatile fatty acids in comparison with the control(P<0.05).The contents of neutral detergent fibre(NDF) and acid detergent fibre(ADF) tended to decrease in silages with cornzyme addition.When increasing the adding rates of cornzyme,the fermentation quality and water soluble carbohydrate content were further increased,however,NDF and ADF decreased.

Key words: Napiergrass, Cornzyme, Fermentation quality, Carbohydrate

摘要： 为评价添加不同比例Cornzyme酶制剂对象草(Pennisetum purpureum Schum.)青贮发酵品质及碳水化合物含量的影响,试验设对照组(CK)和4个不同添加比例的酶处理组(E1,E2,E3,E4),添加比例分别为E1:0.5mL·kg-1鲜草,E2:1.0mL·kg^-1鲜草,E3:1.5mL·kg^-1鲜草,E4:2.0mL·kg^-1鲜草。结果表明:本试验全部处理均有良好的发酵品质,较低的pH值(4.28-3.76)、NH3-N/TN及微量的丙酸、丁酸,较高的乳酸/乙酸比、乳酸含量(45.00-95.41g kg^-1DM)。酶添加显著提高了乳酸/乙酸(P<0.05),乳酸和水溶性碳水化合物含量,同时明显降低了pH、乙酸和总挥发性脂肪酸含量,中性洗涤纤维和酸性洗涤纤维也有一定下降趋势。随着酶添加量的增加会进一步提高发酵品质和水溶性碳水化合物含量(P<0.05),降低中性洗涤纤维和酸性洗涤纤维的含量。

关键词: 象草, Cornzyme酶制剂, 发酵品质, 碳水化合物

CLC Number:

Q946.6

JIA Yan-xia, YU Zhu, SHAO Tao. Effect of Adding Cornzyme on Fermentation Quality and Carbohydrate Contents of Napiergrass Silages[J]. , 2009, 17(1): 121-124.

贾燕霞, 玉柱, 邵涛. 添加酶制剂对象草青贮发酵品质的影响[J]. , 2009, 17(1): 121-124.

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References

[1] Shao T,Zhang L,Shimojo M,Masuda Y.Fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silages treated with encapsulated-glucose,glucose,sorbic acid and pre-fermented juices.Asian-Australasian Journal of Animal Sciences,2007,20(11):1699-1704.
[2] Shao T,Ohba N,Shimojo M,et al.Effects of adding glucose,sorbic acid and pre-fermented juices on the fermentation quality of guineagrass (Panicum maximum Jacq.) silages[J]. Asian-Australian Journal Animal Science,2004,17(6):808-813.
[3] Shao T,Ohba N,Shimojo M,et al.Fermentation quality of forage oat (Avena sativa L.) silages treated with pre-fermented juices,sorbic acid,glucose and encapsulated-glucose[J]. Journal of Faculty of Agriculture,Kyushu University,2003,47(2):341-349.
[4] 郭玉琴,孙彦,滑静,等.纤维素酶对苜蓿草粉营养成分及其利用率的影响[J]. 草地学报,2003,11(1):48-51.
[5] 许庆方,周禾,玉柱,等.贮藏期和添加绿汁发酵液对袋装苜蓿青贮的影响[J]. 草地学报2006,14 (2):129-146.
[6] Shao T,Zhang Z X,Shimojo M,Wang T,Masuda Y.Comparison of fermentation characteristics of Italian ryegrass (Lolium multiflorum Lam.) and guineagrass (Panicum maximum Jacq.) during the early stage of ensiling.Asian-Australasian Journal of Animal Sciences,2005,18(12):1727-1734.
[7] Dean D B,Adesogen A T,Krueger N,Littell R C.Effect of fibrolytic enzymes on the fermentation characteristics,aerobic stability,and digestibility of bermudagrass silage[J]. J.Dairy Sci.,2005,88:994-1003.
[8] Barker S B,Summerson W H.The colorimetric determination of lactic acid in biological material.J.Biol.Chem.,1941,138:535-554.
[9] Madrid J,Martinez-Teruel A,Hernandez F,et al.A comparative study on the determination of lactic acid in silage juice by colorimetric,high-performance liquid chromatography and enzymatic methods[J]. Journal of the Science of Food and Agriculture,1999,79(12):1722-1726.
[10] Weatherburn M W.Phenol-hypochlorite reaction for determination of ammonia[J]. Analytical Chemistry,1976,39(8):971-974.
[11] 丁武蓉,干友民,郭旭生,等.甲酸对二色胡枝子青贮品质的影响.草地学报,2008,16 (1):81-84.
[12] 杨云贵,龙明秀,王莺,等.牧草、玉米青贮和作物秸秆营养价值的洗涤剂法评定[J]. 草地学报,2004,12 (2):132-135.
[13] Colombatto D,Mould F L,Bhat M K,et al.In vitro evaluation of fibrolytic enzymes as additives for maize (Zea mays L.) silage.I.Effects of ensiling temperature,enzyme source and addition level.Anim.Feed Sci.Technol.,2004,111:111-128.
[14] Adogla-Bessa T,Owen E,Adesogan A T.Ensiling of whole crop wheat with cellulase-hemicellulase based enzymes 3.Comparing effects of urea or enzyme treatment on forage composition and stability.Anim.Feed Sci.Technol.,1999,82:51-61.
[15] Nadeau E M,Russell J R,Buxton D R.Intake,digestibility,and composition of orchardgrass and alfalfa silages treated with cellulase,inoculant and formic acid fed to lambs.J.Anim.Sci.,2000,78:2980-2989.
[16] Masuko T,Kodama I,Ohta N.Effects of addition of formic acid or mixture of bacterial inoculant and enzyme on fermentation of orchardgrass (Dactylis glomerata L.),timothy (Phleum pratense L.) and alfalfa (Medicago sativa L.) silages.Grassland Science,1996,42:13-19.
[17] Sheperd A C,Kung Jr.L.An enzyme additive for corn silage:effects on silage composition and animal performance.J.Dairy Sci.,1996,79:1760-1766

Effect of Adding Cornzyme on Fermentation Quality and Carbohydrate Contents of Napiergrass Silages

添加酶制剂对象草青贮发酵品质的影响

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