Effect of Adding Propionic acid and Lactic Acid Bacteria on Fermentation Quality of Italian Ryegrass Silages

doi:10.11733/j.issn.1007-0435.2009.02.006

›› 2009, Vol. 17 ›› Issue (2): 162-165.DOI: 10.11733/j.issn.1007-0435.2009.02.006

Previous Articles Next Articles

Effect of Adding Propionic acid and Lactic Acid Bacteria on Fermentation Quality of Italian Ryegrass Silages

ZHANG Jing¹, YU Zhu², SHAO Tao¹

1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China;
2. Institute of Grassland Science, China Agricultural University, Beijing, 100193, China

Received:2008-06-20 Revised:2008-10-13 Online:2009-04-15 Published:2009-04-15

丙酸、乳酸菌添加对多花黑麦草青贮发酵品质的影响

张静¹, 玉柱², 邵涛¹

1. 南京农业大学, 动物科学技术学院, 江苏, 南京, 210095;
2. 中国农业大学草地研究所, 北京, 100193

通讯作者: 邵涛, E-mail:taoshaolan@yahoo.com.cn
作者简介:张静(1983- ), 女, 四川广元人, 硕士研究生, 研究方向为草业科学
基金资助:
国家自然科学基金面上项目(30771530);“十一五”国家科技支撑计划重点项目(2006BAD16B08-06)

Abstract

Abstract: The experiment was carried out to evaluate the effects of adding propionic acid,lactic acid bacteria,and their combination on the fermentation quality and residual water soluble carbohydrate content of Italian ryegrass(Lolium multiflorum Lam.)silage.The treatments were as follows: Control(no addition);Lactic acid bacteria addition(LAB);Propionic acid addition at 0.4%(PA4);Propionic acid addition at 0.5%(PA5);PA4 ₊ LAB;PA5₊ LAB;all the additions were made on the fresh weight basis of Italian ryegrass.The DM content was not significantly different among all treatments,however,the pH tended to decrease,and the lactic acid content showed significantly(P<0.05)higher in all addition treatments in comparison with control.PA5 and PA5₊LAB treatments showed significantly(P<0.05)higher lactic acid content than the PA4 and PA4₊LAB treatments and there were no significant difference between PA and PA+LAB treatment with the same PA adding rate.PA and PA₊ LAB treatments showed significantly(P<0.05)higher lactic acid/acetic acid than that of control and LAB addition,PA alone significantly(P<0.05)higher than PA+LAB treatment,and there was no significant difference between control and LAB treatment.PA and PA₊ LAB treatments resulted in significantly(P<0.05)lower contents of acetic acid and butyric acid and NH₃-N/total N and significantly(P<0.05)higher contents of propionic acid and residual water soluble carbohydrate in comparison with control and LAB treatment.In summary,addition of propionic acid could depress the undesirable bacterial activities,decrease the water-soluble carbohydrates loss,and promote lactic acid fermentation.

Key words: Italian ryegrass, Silage quality, Propionic acid, Lactic acid bacteria, Residual water soluble carbohydrate

摘要： 本试验旨在评价丙酸、乳酸菌及两者组合添加对意大利黑麦草(Lolium multiflorum Lam.)青贮发酵品质及残余水溶性碳水化合物(WSC)含量的影响。试验共六个处理:对照、乳酸菌(LAB)、0.4%丙酸(PA4)、0.5%丙酸(PA5)、0.4%的丙酸+乳酸菌(PA4₊LAB)和0.5%的丙酸+乳酸菌(PA5₊LAB),以鲜重为基础。结果表明:经过30d青贮发酵,各处理组间DM含量差异不显著,各添加组pH值与对照相比有下降趋势,乳酸含量显著(P<0.05)高于对照,且PA5及其组合添加显著(P<0.05)高于PA4及其组合添加,但在相同PA添加量下,单独或组合添加组间差异不显著。对照组的乳酸/乙酸与LAB组间差异不显著,PA单独或组合添加组显著(P<0.05)高于对照和LAB处理组,但PA单独添加组又显著(P<0.05)高于PA组合添加组;PA单独或组合添加组乙酸、丁酸含量及氨态氮/总氮显著(P<0.05)低于对照和LAB组,丙酸及残留WSC含量显著(P<0.05)高于对照和LAB组;PA单独添加后残留WSC含量明显高于(P<0.05)PA+LAB组合添加;丙酸添加能有效地抑制有害微生物的活性,减少对WSC的消耗,促进乳酸菌发酵,改善意大利黑麦草青贮发酵品质。

关键词: 意大利黑麦草, 青贮品质, 丙酸, 乳酸菌, 残余水溶性碳水化合物

CLC Number:

S816.53

ZHANG Jing, YU Zhu, SHAO Tao. Effect of Adding Propionic acid and Lactic Acid Bacteria on Fermentation Quality of Italian Ryegrass Silages[J]. , 2009, 17(2): 162-165.

张静, 玉柱, 邵涛. 丙酸、乳酸菌添加对多花黑麦草青贮发酵品质的影响[J]. , 2009, 17(2): 162-165.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2009.02.006

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2009/V17/I2/162

References

[1] Shao T,Ohba N,Shimojo M.et al.Dynamics of early fermentation of Italian ryegrass (Lolium multiflorum Lam.)silage[J]. Asian-Australasian Journal of Animal Sciences,2002,15(11):1606-1610
[2] Shao T,Ohba N,Shimojo M,et al.Effect of adding glucose,sorbic acid and pre-fermented juice on the fermentation quality of guineagrass (Panicum maximum Jacq.)silage[J]. AsianAustralasian Journal of Animal Sciences,2004,17 (6):808-813
[3] Shao T,Wang T,Shimojo M,et al.Effect of ensiling density on fermentation quality of guineagrass (Panicum maximum Jacq.)silage during the early stage of ensiling[J]. AsianAustralasian Journal of Animal Sciences,2005,18 (9):1273-1278
[4] Shao T,Zhang Z X,Shimojo M,et al.Comparison of fermentation characteristics of Italian ryegrass (Lolium multiflorum Lam.)and guineagrass (Panicum maximum Jacq.)during the early stage of ensiling[J]. Asian-Australasian Journal of Animal Sciences,2005,18(12):1727-1734
[5] Shao T,Shimojo M,Wang T,et al.Effect of additives on the fermentation quality and residual mono-and di-saccharides compositions of forage Oats (Arena sativa,L.)and Italian ryegrass (Lolium muhiflorum Lam.)silages[J]. Asian-Australasian Journal of Animal Sciences,2005,18(11):1582-1588
[6] Shao T,Zhang L,Shimojo M,et al.Fermentation quality of halian ryegrass (Lolium multiflorum Lam.)silages treated with encapsulated-glucose,glucose,sorbic acid and pre-fermented juices[J]. Asian-Australasian Journal of Animal Sciences,2007,20(11):1699
[7] Kung L Jr,Myers C L,Neylon J M,et al.The effects of buffered propionic acid-based additives alone or combined with microbial inoculation on the fermentation of high moisture corn and whole-crop barley[J]. Journal of Dairy Science,2003,87:1310-1316
[8] Kung L Jr,Ranjit N K.The effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage[J]. Journal of Dairy Science,2001,84:1149-1155
[9] Mills J A,Kung L Jr.The effect of delayed ensiling and application of a propionic acid-based additive of the fermentation of barley silage[J]. Journal of Dairy Science,2002,85:1969-1975
[10] Kung L Jr,Taylor C C,Lynch M P,et al.The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation,aerobic stability and nutritive value for lactating dairy cows[J]. Journal of Dairy Science,2003,86:336-343
[11] Barker S B,Summerson W H.The colorimetric determination of lactic acid in biological material[J]. Journal of Biology Chemistry,1941,138:535-554
[12] Owens V N,Albrecht K A,Muck R E,et al.Protein degradation and fermentation characteristics of red clover and alfalfa silage harvested with varying levels of total nonstructural carbohydrate[J]. Crop Science,1999,39(6):1873-1880
[13] Weather M W.Phenol-hypochlorite reaction for determinations of ammonia[J]. Annual of Chemistry,1967,39:971-974
[14] 杨富裕,周禾,韩建国.添加甲酸对白花草木樨青贮品质的影响[J]. 草地学报,2004,12(1):12-16
[15] 丁武蓉,干友民,郭旭生,等.甲酸对二色胡枝子青贮品质的影响[J]. 草地学报,2008,16(1):81-84
[16] 管武太,Ashbeil G,Hen Y,等.微生物添加剂对青贮高粱发酵品质和稳定性的影响[J]. 中国农业科学,2002,35(11):1401-1405
[17] 许庆方,周禾,玉柱,等.贮藏期和添加绿汁发酵液对袋装苜蓿青贮的影响[J]. 草地学报,2006,14(2):129-133
[18] 孙娟娟.玉柱,薛艳林,等.添加剂对羊草青贮发酵品质和体外消化率的影响[J]. 草地学报,2007,15(3):238-242

Effect of Adding Propionic acid and Lactic Acid Bacteria on Fermentation Quality of Italian Ryegrass Silages

丙酸、乳酸菌添加对多花黑麦草青贮发酵品质的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[2]	DU Zhao-chang, WANG Hong, YAN Yan-hong, MUHAMMAD Tahir, WEI Xiao, ZENG Tai-ru, LI Hong-liang. Effects of Rice Husk and Wheat Bran Addition on Silage Quality of Fresh Corn Straw [J]. Acta Agrestia Sinica, 2021, 29(7): 1549-1554.
[3]	WU Shuo, ZOU Xuan, WANG Ming-ya, ZHANG Qing. Effects of Chenpi Citrus Juice on Fermentation Quality of Stylo and Rice Straw Silage [J]. Acta Agrestia Sinica, 2021, 29(7): 1565-1570.
[4]	FU Zhi-hui, GE Gen-tu, JIA Yu-shan, WANG Zhi-jun, LYU Shi-jie, HOU Mei-ling, NAN Ding-han, YI Ru-le, A Xi-da, JIN Guo. Effects of Cutting Times and Varieties on the Yield and Nutrient Value of Italian Ryegrass Planted in Northern Regions [J]. Acta Agrestia Sinica, 2021, 29(7): 1571-1577.
[5]	LYU Hong-jian, ZOU Xuan, WU Shuo, LI Yun, CHEN Xiao-yang, ZHANG Qing. Effect of Adding Moringa Oleifera Leaves and Lactobacillus farciminis on Quality of Corn Stover Silage [J]. Acta Agrestia Sinica, 2021, 29(6): 1343-1349.
[6]	QIN Wei-na, JIAO Ting, SHI Shang-li, LI Xiong-xiong, GAO Xue-mei, ZHAO Sheng-guo, QI Juan, WANG Zheng-wen. Evaluation of Silage Quality of 10 Green-fed Corn Varieties under Different Hydrothermal Rescource Conditions [J]. Acta Agrestia Sinica, 2021, 29(5): 1070-1079.
[7]	ZHENG Ming-yang, WU Shuo, GUO Xiang, CHEN Xiao-yang, ZHANG Qing. Effects of Lactobacillus and Cellulase on the Silage Quality of Amomum Villosum leaves [J]. Acta Agrestia Sinica, 2021, 29(5): 1113-1117.
[8]	CHEN De-kui, GUO Xiang, ZHENG Ming-yang, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. The Effects of Amomum Villosum Lour. Essential Oil on the Fermentation Quality of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(4): 855-860.
[9]	WANG Li-xue, HAN Jing, CHEN Long-bin, YU Xin-yue, LIU Jing-xi, MA Yi, HUO Wen-juan. Effects of Various Lactic Acid Bacteria Combinations on Bacterial Community Structure of Alfalfa Silage [J]. Acta Agrestia Sinica, 2021, 29(2): 388-395.
[10]	KOU Jiang-tao, CHENG Yu, ZHANG Hai-bo, GUO Dong-sheng, LEI Jia-xing, MENG Jia-qi, LIU Xin, LI Le. Effects of Enzyme on Mixed Silage Quality of Rice Straw and Distiller's Grains [J]. Acta Agrestia Sinica, 2021, 29(2): 396-401.
[11]	SHI Yan-hua, HUANG Yu-ting, SHU Fang-zhi, LU Ye, LIU Da-lin. Alleviation of Exogenous Glutamic Acid on Seedling Growth of Italian Ryegrass under Aluminum Stress [J]. Acta Agrestia Sinica, 2020, 28(6): 1605-1614.
[12]	BAI Chun-sheng, TONG Ming-hao, ZHAO Meng-meng, SUN Juan-juan, XUE Yan-lin, YU Zhu. The Effects of Nitrogen Fertilization and Cutting Height on Silage Fermentation Quality and Forage Value of Sorghum bicolor×S. sudanense [J]. Acta Agrestia Sinica, 2020, 28(5): 1421-1426.
[13]	CHEN Die, LIN Kai-cheng, LIN Qin, CHEN Xia, CHEN Xinzhu. Isolation and Identification of Salt-Tolerant Lactic Acid Bacteria in Spartina anglica Hubb. and Its Effect on the Quality of Silage [J]. Acta Agrestia Sinica, 2020, 28(2): 565-570.
[14]	LI Long-xing, WANG Yi-ni, YANG Xue-dong, LI Jun, GONG Zheng-fa, FRASER George Harrison, CHEN Xiu-hua, HUANG Wen-hu, LIANG Xian-yi. Effects of Wilting and Adding Lactobacillus on Fermentation Quality of Coix lacryma-jobi Silage [J]. Acta Agrestia Sinica, 2019, 27(4): 1102-1106.
[15]	MU Lin, LI Shun, ZENG Ning-bo, CHEN Gui-hua, ZHANG Zhi-fei, YE Zhi-gang. Effect of Adding Molasses or Lactic Acid Bacteria on Quality of Mixed Silage of Amaranth and Rice Straws [J]. Acta Agrestia Sinica, 2019, 27(2): 482-487.