Optimization of Culture Condition for Hyphae Growth and Toxin Production of Alternaria tenuis of Onobrychis viciaefolia Scop.

doi:10.11733/j.issn.1007-0435.2009.02.023

›› 2009, Vol. 17 ›› Issue (2): 255-258.DOI: 10.11733/j.issn.1007-0435.2009.02.023

Previous Articles Next Articles

Optimization of Culture Condition for Hyphae Growth and Toxin Production of Alternaria tenuis of Onobrychis viciaefolia Scop.

GUO Xia, HUANG Xiao-ya, LI Rui-hua, ZHU Jian-lan

Key laboratory of Grassland Ecosystem of Ministry of Education & Sino-US Centers for Grazingland Ecosystem Sustainability, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province, 730070, China

Received:2008-09-19 Revised:2009-01-12 Online:2009-04-15 Published:2009-04-15

红豆草黑腐病菌菌丝生长和产毒培养条件的优化

郭霞, 黄晓亚, 李瑞华, 朱建兰

甘肃农业大学草业学院, 草业生态系统教育部重点实验室(甘肃农业大学), 中美草地畜牧业可持续发展研究中心, 兰州, 730070

通讯作者: 朱建兰, E-mail:zhujl@gsau.edu.cn
作者简介:郭霞(1984- ), 女, 甘肃徽县人, 硕士研究生, 研究方向为植物病理学, E-mail:guoxia1984@yahoo.cn
基金资助:
甘肃农业大学草业学院国家级重点学科学术骨干科研项目

Abstract

Abstract: Factors affecting the hyphae growth and toxin production of Alternaria tenuis isolated from black rot of sainfoin were studied.The results show that the pathogen could grow and product toxin in six kinds of fluid media,but the optimal liquid culture media for hyphae growth and toxin production was PSK.During the cultural period of 1 to 19 days,the hyphae growth and phytotoxin production were detected and reached to maximum on 13th day,and there were a positive correlation between mycelial growth and toxin production.The pathogen could grow and produce toxin under 5 to 40℃,and the optimum temperature for mycelial growth and toxin production was 25℃ and 15℃,respectively.In a pH range of 3 to 11,the pathogen could grow and produce toxin with favorable environment of acid condition,the optimal pH value for hyphae growth and toxin production was 4 and 6,respectively.

Key words: Alternaria tenuis, Hyphae growth, Toxin production, Culture condition

摘要： 从红豆草自然发生的黑腐病病斑上分离得到细链格孢菌(Alternaria tenuis)菌株,对该病菌的菌丝生长及其产毒条件进行了研究。结果表明,病原菌在供试的6种培养液中均能生长并且产毒,但在PSK培养液中生长及产毒最好;在1-19d的培养时间内该病菌均可以生长及产生毒素,而在13 d时菌丝生长量及其产毒能力均达到最大,菌丝生长与产毒能力间显著正相关;该病原菌在5-40℃的范围内均可生长并产生毒素,但菌丝生长的最适温度为25℃,而最适合产毒的温度为15℃;在pH值为3~11的范围内,该病菌均能生长并产毒,而且偏酸性条件有利于菌丝的生长及其毒素的产生,菌丝生长和产毒的最佳pH值分别为4和6。

关键词: 黑腐病菌, 菌丝生长, 产毒, 培养条件

CLC Number:

S812
Q939.96

GUO Xia, HUANG Xiao-ya, LI Rui-hua, ZHU Jian-lan. Optimization of Culture Condition for Hyphae Growth and Toxin Production of Alternaria tenuis of Onobrychis viciaefolia Scop.[J]. , 2009, 17(2): 255-258.

郭霞, 黄晓亚, 李瑞华, 朱建兰. 红豆草黑腐病菌菌丝生长和产毒培养条件的优化[J]. , 2009, 17(2): 255-258.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2009.02.023

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2009/V17/I2/255

References

[1] 陈宝书.红豆草产量和营养成分的研究[J]. 中国草原,1983,(2):36-38
[2] 朱建兰.黑腐病对红豆草茎叶蛋白质、氨基酸等含量的影响[J]. 草业科学,2006,23(4):67
[3] 张天宇.中国真菌志(链格孢属)[M]. 北京:科学出版社,2003.38-42
[4] 万佐玺,强胜,李扬汉.链格孢菌寄主选择性毒素的研究现状[J]. 湖北民族学院学报(自然科学版),2001,19(4):19-22
[5] 童蕴慧.徐敬友,袁素玲.番茄早疫病病菌培养滤液的毒性及不同品种对滤液的敏感性[J]. 江苏农业学报,1997,13(4):250-251
[6] 万佐玺,强胜,徐尚成,等.链格孢菌的产毒培养条件及其毒素的致病范围[J]. 中国生物防治,2001,17(1):10-15
[7] 李荣金,强胜.百日草链格孢菌粗毒素的生产、提取及稳定性的研究[J]. 中国生物工程杂志,2006,26(8):67-71
[8] 雷玉明,张建文,草本植物三种真菌新病害[J]. 草地学报,2006,14(3):284-286
[9] 方中达.植病研究法[M]. 北京:中国农业出版社,1998.122-125
[10] 郑莉,黄俊斌,向培建.高羊茅叶斑病病原鉴定及生物学特性[J]. 草地学报,2006,14(4):315-318
[11] 万佐玺,周光来,强胜.链格孢菌产毒菌株及产毒培养基的筛选[J]. 湖北民族学院学报(自然科学版),2001,19(4):7-10
[12] 樊慕贞,董金皋,杨俊国.等.白菜黑斑病菌菌丝生长和毒素产生条件的研究[J]. 河北农业大学学报,1995,18(4):21-27
[13] 王进,韩多红,陈叶,等.环境因子对苦豆子种子萌发和幼苗生长的影响[J]. 草地学报,2007,15(3):259-262
[14] 董金皋,朱晞.真菌毒素生物测定方法研究概况Ⅰ[J]. 河北农业大学学报,1992,15(4):99-103
[15] 唐启义,冯明光.实用统计分析及其计算机处理平台[M]. 北京:中国农业出版社,2002.56-109
[16] Miller J D.Production of deoxynivalenol and related compounds in liquid culture by Fusarium graminearum[J]. Can.J.Micrbiol.,1983.12(9):1171-1178
[17] 徐敬友,张华东,张红,等.立枯丝核菌毒素的产生及与致病力的关系[J]. 扬州大学农业与生命科学版,2004,25(2):61-64
[18] 吴蔼民,夏正俊,傅正擎,等.培养条件对棉花黄萎病菌毒素产生的影响[J]. 江苏农业学报,1999,15(2):96-99
[19] 周慧,郜瑞敏,孙炳剑,等.小麦黑胚病菌链格孢产毒培养条件及其毒素的致病力测定[J]. 河南科学,2008,26(3),294-297
[20] Pathre S V,Miroeha C J.Analysis of deoxyniralenol from culture of Fusarium species[J]. Applied and Environ Microbiology,1978,5:992-994
[21] 拓植尚志.链格孢菌产生寄主特异性毒素的代谢调节Ⅰ.高温对孢子萌发时生成毒素的抑制[J]. 日本植物病理学会报,1984,50(2),189-196
[22] 张志强,程智慧.沈永杰.大蒜叶枯病菌毒素产生条件的研究[J]. 西北农林科技大学学报(自然科学版),2007,35(12):186-190

Optimization of Culture Condition for Hyphae Growth and Toxin Production of Alternaria tenuis of Onobrychis viciaefolia Scop.

红豆草黑腐病菌菌丝生长和产毒培养条件的优化

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments