Cytological Effect of 60Coγ Ray Irradiation on Buchloe dactyloides Dry Seeds

doi:10.11733/j.issn.1007-0435.2009.06.018

›› 2009, Vol. 17 ›› Issue (6): 795-798.DOI: 10.11733/j.issn.1007-0435.2009.06.018

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Cytological Effect of ⁶⁰Coγ Ray Irradiation on Buchloe dactyloides Dry Seeds

LI Jian¹, WANG Jian², ZHAO Zhi-tong³, ZHANG Yun-wei¹

1. Institute of Grassland Science, China Agricultural University, Beijing 100193, China;
2. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
3. College of Agronomy, Inner Mongolia University for Nationality, Tongliao, Inner Mongolia Autonomous Region 028043, China

Received:2009-05-14 Revised:2009-07-08 Online:2009-12-15 Published:2009-12-15

⁶⁰Coγ射线辐照野牛草干种子的细胞学效应

李健¹, 王健², 赵智同³, 张蕴薇¹

1. 中国农业大学草地研究所, 北京, 100193;
2. 甘肃农业大学草业学院, 兰州, 730070;
3. 内蒙古民族大学农学院, 通辽, 028043

通讯作者: 张蕴薇,E-mail:zywei@126.com
作者简介:李健(1985- ),男,湖南永兴县人,硕士研究生,研究方向为牧草与草坪草育种,E-mail: lijian4531@163.com
基金资助:
十一五科技支撑项目:(2006BAD01A19-3),(2008BADB3B04),(2008BAD97B07);863项目(2007AA100105)

Abstract

Abstract: The irradiation of ⁶⁰Coγ ray can induce different types of variation and irradiation breeding has been applied to turfgrass variety improvement as one effective method.The cytological effects of 5 kinds of dosages of ⁶⁰Coγ ray on Buchloe dactyloides(Nutt) Engelm.seeds were evaluated and the results show that the γ ray irradiation inhibited the mitoses of root tip cells.And the mitotic index of root tip cells decreased along with the increased dosage.Furthermore,the γ ray irradiation induced the chromosomal and nuclear aberration,such as bridge,chromosome fragment,chromosome conglutination,chromosome dissociation,draggling chromosome,single micronucleus,double micronucleus,multi-micronucleus,and small micronucleus in root tip cells of B.dactyloides.The higher the γ-rays irradiation dosage,the higher the rate of aberration from the irradiation dosage of 0 to 2000 Gy;all the aberration rates reached to the maximum at 2000 Gy.

Key words: Buchloe dactyloides(Nutt) Engelm., ⁶⁰Coγ ray, Mitotic index, Nuclear aberration, Chromosomal aberration

摘要： 通过5种不同剂量的⁶⁰Coγ射线对野牛草(Buchloe dactyloides(Nutt) Engelm.)种子的辐射诱变效应研究表明,γ射线辐照能抑制野牛草根尖细胞的有丝分裂.随γ射线辐射剂量的增加,根尖细胞有丝分裂指数呈下降趋势.γ射线辐照能诱发根尖细胞的染色体畸变和核畸变,产生桥、染色体断片、粘连染色体、游离染色体、落后染色体、单微核、双微核、多微核、小核等多种畸变类型.在0～2000 Gy 范围内,随剂量的增加各种畸变率不断提高,至2000Gy 辐照剂量时,各畸变率达到最大.

关键词: 野牛草, γ射线, 细胞有丝分裂指数, 核畸变, 染色体畸变

CLC Number:

S335.21
S812

LI Jian, WANG Jian, ZHAO Zhi-tong, ZHANG Yun-wei. Cytological Effect of ⁶⁰Coγ Ray Irradiation on Buchloe dactyloides Dry Seeds[J]. , 2009, 17(6): 795-798.

李健, 王健, 赵智同, 张蕴薇. ⁶⁰Coγ射线辐照野牛草干种子的细胞学效应[J]. , 2009, 17(6): 795-798.

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Cytological Effect of ⁶⁰Coγ Ray Irradiation on Buchloe dactyloides Dry Seeds

⁶⁰Coγ射线辐照野牛草干种子的细胞学效应

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