Study on Culture Condition of Bacillus mucilaginosus Isolated from Rhizosphere of Kentucky Bluegrass

doi:10.11733/j.issn.1007-0435.2009.06.024

›› 2009, Vol. 17 ›› Issue (6): 822-825.DOI: 10.11733/j.issn.1007-0435.2009.06.024

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Study on Culture Condition of Bacillus mucilaginosus Isolated from Rhizosphere of Kentucky Bluegrass

ZHAO Yan¹, ZHANG Xiao-bo², GUO Wei³, HONG Jian-pin¹

1. College of Resources & Environment, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. College of Agriculture, Hainan University, Danzhou, Hainan Province 571737, China;
3. Jincheng Agro-technical Extension Centre, Jincheng, Shanxi Province 040026, China

Received:2008-12-11 Revised:2009-10-10 Online:2009-12-15 Published:2009-12-15

草地早熟禾根际胶质芽孢杆菌培养条件研究

赵艳¹, 张晓波², 郭伟³, 洪坚平¹

1. 山西农业大学资环学院, 山西, 030801;
2. 海南大学农学院, 海南, 571737;
3. 晋城市农业技术推广站, 晋城, 040026

通讯作者: 洪坚平,E-mail:yanbo315@126.com
作者简介:赵艳(1981- ),女,汉族,山西太谷人,讲师,博士研究生,研究方向为微生物相关方面,E-mail:angiaoo@126.com
基金资助:
海南省自然科学基金(309009);山西农业大学科技创新基金(2005015)

Abstract

Abstract: Culture conditions and typical growth curve of three Bacillus mucilaginosus strains isolated from rhizosphere of Kentucky bluegrass in September 2007 were studied.The results show that the strains could grow under 10℃ to 45℃,the optimum temperature was from 35℃ to 40℃,the optimum initial pH range was 7.5 to 8.0,and the optimum ventilation volume of strain K7,K3 and K5 were 220,100,and 150 mL,respectively.Based on the above results,the typical strain growth curves were determined.The experimental data would be significance for the understanding of growth pattern and utilization as biological fertilizer of these strains.

Key words: Kentucky bluegrass, Bacillus mucilaginosus, Culture condition, Typical growth curve

摘要： 于2007年9月从草地早熟禾(Poa pratensis L.)根际土壤中筛选得出3株胶质芽孢杆菌(Bacillus mucilaginosus)为试验材料,对其培养条件以及典型生长曲线开展研究.结果表明,菌株在10℃～5℃内均可生长,最适生长温度在35℃～0℃;最适生长初始pH值在7.5～8.0;菌株K7、K3、K5的最佳通气量分别为220 mL、100mL和150mL;并在上述基础上经过进一步试验得到了菌株的典型生长曲线.试验数据对于了解掌握菌株的生长规律以及作为生物菌肥加以利用具有重要的意义.

关键词: 草地早熟禾, 胶质芽孢杆菌, 培养条件, 典型生长曲线

CLC Number:

S154.37

ZHAO Yan, ZHANG Xiao-bo, GUO Wei, HONG Jian-pin. Study on Culture Condition of Bacillus mucilaginosus Isolated from Rhizosphere of Kentucky Bluegrass[J]. , 2009, 17(6): 822-825.

赵艳, 张晓波, 郭伟, 洪坚平. 草地早熟禾根际胶质芽孢杆菌培养条件研究[J]. , 2009, 17(6): 822-825.

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Study on Culture Condition of Bacillus mucilaginosus Isolated from Rhizosphere of Kentucky Bluegrass

草地早熟禾根际胶质芽孢杆菌培养条件研究

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