Biological Characteristics of Chinese Strain of Verticillium albo-atrum from Alfalfa

doi:10.11733/j.issn.1007-0435.2010.02.025

›› 2010, Vol. 18 ›› Issue (2): 274-279.DOI: 10.11733/j.issn.1007-0435.2010.02.025

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Biological Characteristics of Chinese Strain of Verticillium albo-atrum from Alfalfa

CHEN Jing¹, BAI Ying-wen¹, YANG Ji-juan¹, CHEN Wei-ming², ZHANG Li³, HU Xiao-ping ¹

1. College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. Yili Vocational and Technical College, Yining, Xinjiang Uygur Autonomous Region 835000, China;
3. Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region 832003, China

Received:2009-02-13 Revised:2010-01-19 Online:2010-04-15 Published:2010-04-15

苜蓿黄萎病菌中国菌株生物学特性研究

陈婧¹, 白应文¹, 杨继娟¹, 陈卫民², 张莉³, 胡小平¹

1. 西北农林科技大学植保学院与陕西省农业分子生物学重点实验室, 陕西, 杨凌, 712100;
2. 新疆伊犁职业技术学院, 新疆, 伊宁市, 835000;
3. 石河子大学, 新疆, 石河子市, 832003

通讯作者: 胡小平,E-mail:xphu@nwsuaf.edu.cn
作者简介:陈婧(1983- ),女,河北香河县人,硕士,研究方向为植物病理学研究,E-mail:chj79919@126.com
基金资助:
教育部新世纪优秀人才支持计划资助(NCET-07-0698);高等学校学科创新引智计划(No.B07049)资助

Abstract

Abstract: In order to nuderstand the biological characteristics of Verticillium albo-atrum Reinke et Berthold in China,the isolation,identification and biological characteristics of the alfalfa verticillium wilt pathogen were studied from 2007 to 2008.Through tissue plating,one pathogen was isolated from diseased alfalfa plant in Xinyuan County of Xinjiang Uygur Autonomous Region.The pathogen was identified as V.albo-atrum VA001 based on the Koch's pustule,morphological characteristics and the sequence of ribosomal DNA-5.8S/ITS.The study results of the biological characteristics of VA001 show that melon lactose agar(MA),potato sugar agar(PSA),potato dextrose agar(PDA) media were the best medium for the strain growth and the PDA,MA,and plum culture agar(PCA) media for sporulation.Among the tested carbon and nitrogen resources,maltose,lactose,mannitolum,lysine,beef extract,aminoacetic acid,and histidine were beneficial for mycelial growth and sucrose,fructose,lactose,sodium nitrate,and alanine for sporulation.For growth and sporulation of the strain,the optimal pH was 7.0-9.5 and temperature was 25℃.

Key words: Verticillium albo-atrum Reinke et Berthold, Verticillium wilt of alfalfa, Internal transcribed spacer(ITS) of ribosomal DNA, Biological characteristics

摘要： 为了解我国苜蓿黄萎病菌的生物学特性,于2007-2008年间对苜蓿黄萎病菌进行了分离、鉴定及生物学特性研究。采用常规组织分离法从新疆伊犁地区新源县苜蓿黄萎病标样中分离到一种真菌(VA001),依据Koch′s法则,并结合形态学、培养特性及ITS序列分析,鉴定为黑白轮枝菌(Verticillium albo-atrum Reinke et Berthold)。适合该菌生长的培养基有甜瓜培养基、马铃薯蔗糖培养基、马铃薯培养基,适合产孢的培养基有马铃薯葡萄糖培养基、甜瓜培养基、梅干培养基。在碳源和氮源中,麦芽糖(Maltose)、乳糖(Lactose)、甘露醇(Mannitolum)、赖氨酸(Lysine)、牛肉膏(Beef extract)、氨基乙酸(Aminoacetic acid)、组氨酸(Histidine)有利于病菌的生长;蔗糖(Sugar)、果糖(Fructose)、乳糖(Lactose)、硝酸钠(Sodium nitrate)、丙氨酸(Alanine)有利于病菌的产孢。苜蓿黄萎菌生长和产孢的适宜pH值为7.0-9.5,温度为25℃。

关键词: 黑白轮枝菌, 苜蓿黄萎病, 核糖体DNA-ITS, 生物学特性

CLC Number:

S432.44

CHEN Jing, BAI Ying-wen, YANG Ji-juan, CHEN Wei-ming, ZHANG Li, HU Xiao-ping . Biological Characteristics of Chinese Strain of Verticillium albo-atrum from Alfalfa[J]. , 2010, 18(2): 274-279.

陈婧, 白应文, 杨继娟, 陈卫民, 张莉, 胡小平. 苜蓿黄萎病菌中国菌株生物学特性研究[J]. , 2010, 18(2): 274-279.

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Biological Characteristics of Chinese Strain of Verticillium albo-atrum from Alfalfa

苜蓿黄萎病菌中国菌株生物学特性研究

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