Abstract: The effects of exogenous nitric oxide on seed germination and seedling growth in tall fescue under salt stress were studied with TP sowing method in September,2009.The results showed that tall fescue seed germination and seedling growth were improved under salt stress adding with 100 μM exogenous sodium nitroprusside(SNP).Seed germination and biomass increased of 107.6% and 24.5% in Arid3,and increased of 94.5% and 14.5% in Houndog5,respectively.These responses could be reduced by the treatment with the 1% NO scavenger,haemoglobin.SNP can generate products,such as NaNO₂ and NO,and the former had not remarkable effects on seed germination and seedling growth of tall fescue under salt stress.So,it was suggested exogenous NO could protect seed germination and seedling growth of tall fescue from the salt stress in some degree.

Key words: Nitric oxide, Seed germination, Seedling growth, Salt stress, Tall fescue

摘要： 为探讨盐胁迫下草坪草种子萌发如何受到其他外源物质的影响,于2009年9月采用TP发芽法,研究外源一氧化氮(Nitric oxide,NO)供体对盐胁迫下的高羊茅(Festuca arundinacea)种子萌发和幼苗生长的作用。结果表明:100μM的NO供体硝普钠(Sodium nitroprusside,SNP)可提高盐胁迫下高羊茅2个品种的种子发芽率和生物量,Arid3分别提高107.6%和24.5%,而Houndog5则提高94.5%和14.4%;施用0.1%的NO清除剂牛血红蛋白(Haemoglobin)具有削弱外源NO供体促进种子的发芽率和生物量的作用;NO供体SNP的另一光分解产物亚硝酸钠(NaNO²)没有提高盐胁迫下高羊茅种子的发芽和生物量。因此,盐胁迫下外源NO对高羊茅种子的萌发及幼苗生长具有保护作用。

关键词: 一氧化氮, 种子萌发, 幼苗生长, 盐胁迫, 高羊茅