Effect of Associative Nitrogen-fixing Biofertilizer Produced with Different Carriers on Growth and Nitrogen Fixing Ability of Hordeum vulgare var. nudum Hook.f.

doi:10.11733/j.issn.1007-0435.2010.03.021

›› 2010, Vol. 18 ›› Issue (3): 426-430.DOI: 10.11733/j.issn.1007-0435.2010.03.021

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Effect of Associative Nitrogen-fixing Biofertilizer Produced with Different Carriers on Growth and Nitrogen Fixing Ability of Hordeum vulgare var. nudum Hook.f.

ZHANG Kun^1,2, YAO Tuo¹, ZHANG De-gang¹, XIN Guo-rong ²

1. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province, 730070, China;
2. State Key Laboratory for Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong Province, 510275, China

Received:2009-10-12 Revised:2009-11-19 Online:2010-06-15 Published:2010-06-15

不同剂型联合固氮菌肥对青稞促生效应和固氮能力研究

张堃^1,2, 姚拓¹, 张德罡¹, 辛国荣², 师尚礼¹

1. 甘肃农业大学草业学院, 甘肃, 兰州, 730070;
2. 有害生物控制与生物资源利用国家重点实验室, 中山大学生命科学学院, 广东, 广州, 510275

通讯作者: 姚拓,E-mail:yaotuo@gsau.edu.cn
作者简介:张堃(1979- ),男,甘肃天水人,博士研究生,研究方向为土壤肥料与环境生态学,E-mail:gszhangkun@163.com
基金资助:
国家自然基金(30960265);农业部燕麦产业体系和现代农业产业技术体系建设专项资金资助

Abstract

Abstract: The associative nitrogen-fixing biofertilizer as a new green fertilizer has been paid more attention recently.The effect of biofertilizer produced with associative nitrogen-fixing bacteria and different carriers(LB liquid culture medium and peat) on the growth and nitrogen fixing ability of Hordeum vulgare var.nudum Hook.f.was studied through pot experiment in greenhouse.¹⁵N isotope dilution method was employed with 7 treatments,including CK1(peat),CK2(half application rate of ¹⁵N labeled urea),1N(whole application rate of urea),1/2NL(half application rate of ¹⁵N labeled urea+liquid biofertilizer),NL(liquid biofertilizer),1/2NS(half application rate of ¹⁵N labeled urea+peat biofertilizer),and NS(peat biofertilizer).The results indicate that the associative nitrogen-fixing biofertilizer accorded with the quality criterion of agricultural microbial fertilizer of China(GB 20287-2006) and the number of effective bacteria in peat biofertilizer was much higher than liquid biofertilizer.The effect of the biofertilizer added with half application rate of chemical nitrogen fertilizer(110 kg·hm^-2) on plant height,aboveground biomass,underground biomass,and root/shoot ratio was similar to the whole application rate of chemical nitrogen fertilizer(220 kg·hm^-2),suggesting that the half chemical nitrogen fertilizer could be saved.The nitrogen fixing percentage and amount of peat biofertilizer and liquid biofertilizer were 17.44%,14.03% and 22.87 kg·hm^-2,21.29 kg·hm^-2,respectively.The peat biofertilizer had better growth performing effect than liquid biofertilizer.

Key words: Hordeum vulgare L.var.nudum Hook.f., Associative nitrogen-fixing biofertilizer, Growth promoting effect, Nitrogen fixing percentage, Nitrogen fixing amount

摘要： 利用联合固氮菌(Associative nitrogen-fixing bacteria)制作菌肥,设7个处理:CK1(基质对照,泥炭拌种)、CK2(半量¹⁵N尿素)、1N(全量氮肥)、1/2NL(半量¹⁵N尿素₊液态菌肥)、NL(液态菌肥)、1/2NS(半量¹⁵N尿素₊固态菌肥)、NS(固态菌肥),通过温室盆栽实验,利用¹⁵N稳定性同位素稀释法,测定其对青稞(Hordeum vulgarevar.nudum Hook.f.)生长及固氮能力的影响。结果表明,本研究研制的联合固氮菌肥符合《农用微生物菌剂》质量标准(GB20287-2006),且固体菌肥的有效活菌数在检测的各个时期都高于液体菌肥。对于株高、地上生物量、地下生物量、根冠比来说,联合固氮菌肥与半量氮肥(110kg·hm^-2)配合施用,表现出与全量氮肥相近的促生效果,即可节约近一半的氮肥用量(110kg·hm^-2);固体菌肥和液体菌肥的固氮百分率和固氮量分别为17.44%、14.03%和22.87 kg·hm^-2、21.29 kg·hm^-2。并且,固体菌肥对青稞生长的促进作用优于液体菌肥。

关键词: 青稞, 联合固氮菌肥, 促生效应, 固氮百分率, 固氮量

CLC Number:

ZHANG Kun, YAO Tuo, ZHANG De-gang, XIN Guo-rong . Effect of Associative Nitrogen-fixing Biofertilizer Produced with Different Carriers on Growth and Nitrogen Fixing Ability of Hordeum vulgare var. nudum Hook.f.[J]. , 2010, 18(3): 426-430.

张堃, 姚拓, 张德罡, 辛国荣, 师尚礼. 不同剂型联合固氮菌肥对青稞促生效应和固氮能力研究[J]. , 2010, 18(3): 426-430.

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References

[1] Doereiner J,Day J M.Associative symbioses in tropical grasses:characterisation of micro-organisms and dinitrogen-fixing sites[R]. In:Newton W E,Nyman C J.(Eds.) Proc.1st Intern.Symp.on Nitrogen Fixation,Washington State University Press,1976.518-538
[2] Dobbelaere S,Croonenborghs A,Thys A et al.Responses of agronomically important crops to inoculation with Azospirillum[J]. Aust.J.Plant Physiol.,2001,28:871-879
[3] Dobbelaere S,Vanderleyden J,Okon Y.Plant growth-promoting effects of diazotrophs in the rhizosphere[J]. Critical Reviews in Plant Sciences,2003,22(2):134-177
[4] 《西藏自治区农牧业特色产业发展研究》课题组.西藏自治区农牧业特色产业发展研究[M]. 中国农业科学技术出版社,2003.109-191
[5] Yao T,Yasmin S and Hafeez F Y.Potential role of rhizobacteria isolated from northwestern China for enhancing wheat and oat yield[J]. The Journal of Agricultural Science,2008 (1):49-56
[6] 朱颖,姚拓,李玉娥,等.红三叶根际溶磷菌分离及其溶磷机制初探[J]. 草地学报,2009,(2):259-263
[7] 《农用微生物菌剂》质量标准[S]. GB 20287-2006
[8] 章家恩.生态学常用实验研究方法与技术[M]. 北京:化学工业出版社,2006.62-78
[9] Use of isotope and radiation methods in soil and water management and crop nutrition[M]. Vienna:International Atomic Energy Agency (IAEA),2001.27-29
[10] 李凤霞,姚拓,张德罡.植物生长促生菌浸种对高寒地区燕麦生长及固氮的效应[J]. 草业科学,2005,23 (1):22-25
[11] 席琳乔,姚拓,张德罡.固氮菌对燕麦不同生育期促生作用的研究[J]. 草业学报,2007,16 (3):38-42
[12] Yanni Y G,Rizk R Y,El-Fattah F K.The beneficial plant growth-promoting association of Rhizobium leguminosarum bv.trifolii with rice roots[J]. Austrian Journal of Plant Physiology,2001,28:845-870
[13] Govindarajan M,Balandreau J,Muthukumarasamy R et al.Improved yield of micropropagated sugarcane following inoculation by endophytic Burkholderia vietnamiensis[J]. Plant Soil,2006,280:239-252
[14] Okon Y,Labandera-Gonzalez C A.Agronomic applications of Azospirillum:an evaluation of 20 years worldwide field inoculation[J]. Soil Biology and Biochemistry,1994,26:1591-1601
[15] Unkovich M J,Pate J S.An appraisal of recent field measurements of symbiotic N2 fixation by annual legumes[J]. Field Crops Research,2000,65:211-228
[16] James E K.Nitrogen fixation in endophytic and associative symbiosis.Field Crops Research,2000,65:197-209
[17] Oliveira A M,Urquiaga S,Dobereiner J,Baldani J I.The effect of inoculating endophytic N2-fixing bacteria on micropropagated sugarcane plants[J]. Plant Soil,2002,242:205-215
[18] de Oliveira A L,de Canuto E L,Urquiaga S.Yield of micropropagated sugarcane varieties in different soil types following inoculation with diazotrophic bacteria[J]. Plant Soil,2006,284:23-32

Effect of Associative Nitrogen-fixing Biofertilizer Produced with Different Carriers on Growth and Nitrogen Fixing Ability of Hordeum vulgare var. nudum Hook.f.

不同剂型联合固氮菌肥对青稞促生效应和固氮能力研究

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