Effect of Enhanced UV-B Radiation on Photosynthesis and Photosynthetic Pigments in Alpine Plants Saussurea superba

doi:10.11733/j.issn.1007-0435.2010.05.001

›› 2010, Vol. 18 ›› Issue (5): 607-614.DOI: 10.11733/j.issn.1007-0435.2010.05.001

Effect of Enhanced UV-B Radiation on Photosynthesis and Photosynthetic Pigments in Alpine Plants Saussurea superba

SHI Sheng-bo^1,2, SHANG Yan-xia², ZHU Peng-jin², ZHANG De-gang¹

1. College of Pratacultural Science, Key Laboratory of Grassland Ecosystem(GAU) in Ministry of Education, Sino-U.S.Centers for Grazingland Ecosystem Sustainability, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Key Laboratory of Adaptation and Evolution of Plateau Biology, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810001, China

Received:2010-07-19 Revised:2010-09-10 Online:2010-10-15 Published:2010-10-15

增补UV-B辐射对高山植物美丽风毛菊光合作用和光合色素的影响

师生波^1,2, 尚艳霞², 朱鹏锦², 张德罡¹

1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室(甘肃农业大学), 中-美草地畜牧业可持续发展研究中心, 甘肃兰州730070;
2. 中国科学院西北高原生物研究所, 高原生物适应与进化重点实验室, 青海, 西宁, 810001

作者简介:师生波(1963- ),男,青海西宁人,副研究员,主要从事光合生理和能量耗散方面的工作,E-mail:sbshi@nwipb.cas.cn
基金资助:
国家自然科学基金课题项目(30670307)(30570270)(30170154);国际科技合作重点项目计划(2002CB714006)资助

Abstract

Abstract: Strong solar UV-B radiation is the main environmental stress factors in Qinghai-Tibet region.A UV-B-supplementation study was performed in alpine Kobresia humilis meadow using cellulose diacetate filtered fluorescence UV-B lamps,comparing to the control lamps with a Mylar film in order to remove UV-B emission.Results from alpine plant Saussurea superba indicated that net photosynthetic rate,P_n,was enhanced after supplementing solar UV-B intensity,resulting from the opening of stomatal conductance.However,Φ_PSⅡ showed a decrease after 11 and 16 days of treatment,suggesting that accumulation of damage from UV-B radiation could reduce PSⅡ photochemistry efficiency.Although there was an increase of photosynthetic pigments within the first 10 days of treatment,they decreased by the end of experiment indicating that UV-B radiation causes photo-damage of photosynthetic pigments and that the initial increase of its contents was mainly due to the increase of leaf thickness.UV-B-absorbing compounds were not altered after enhancement of UV-B radiation suggesting that there were higher levels of flavonoid compounds in the epidermal layer of S.superba acting to protect photosynthetic function from UV-B damage.In summary,the short-term enhanced UV-B radiation was beneficial for Gs and photosynthetic pigments,but there were still negative effects on photosynthesis function of this native alpine plant.

Key words: Alpine plant, Photosynthesis, Qinghai-Tibet plateau, Photosynthetic pigments, UV-B radiation

摘要： 强太阳紫外线-B(UV-B)辐射是青藏高原的主要环境胁迫因子,以高山植物美丽风毛菊(Saussurea superba)为材料,在2008和2009年植物生长盛期的7,8月份,采用选择性过滤膜和UV-B荧光灯管组合的方法研究了净光合速率(P_n)、稳态PSⅡ光化学效率、光合色素和紫外线吸收物质对强UV-B辐射的响应,探讨了典型高山植物的UV-B适应特性。结果表明:增补UV-B辐射能引起P_n和气孔导度(G_s)的增加;随处理时间的延长,第11和16天PSⅡ实际量子效率(Φ_PSⅡ)呈下降趋势,说明UV-B辐射对高山植物叶片光合机构仍具有负作用,PSⅡ光化学效率的降低反映了UV-B辐射效应的积累。增补初期光合色素有增加的趋势,但随处理时间的延长最终呈降低趋势,表明UV-B辐射对光合色素的光破坏是本质的,而基于叶片厚度增加导致的光合色素含量升高是一种表象。增补UV-B处理没有引起紫外线吸收物质含量的变化,表明高山植物叶表皮层丰富的黄酮类物质能有效保护光合机构,受环境UV-B辐射波动的影响较少。综上表明:增补UV-B后叶片G_s的增加以及单位叶片面积光合色素增加有利于P_n的提高,但青藏高原强太阳UV-B辐射对高山植物美丽风毛菊的光合生理过程仍具有潜在的负效应。

关键词: 高山植物, 光合作用, 青藏高原, 光合色素, UV-B辐射

CLC Number:

SHI Sheng-bo, SHANG Yan-xia, ZHU Peng-jin, ZHANG De-gang. Effect of Enhanced UV-B Radiation on Photosynthesis and Photosynthetic Pigments in Alpine Plants Saussurea superba[J]. , 2010, 18(5): 607-614.

师生波, 尚艳霞, 朱鹏锦, 张德罡. 增补UV-B辐射对高山植物美丽风毛菊光合作用和光合色素的影响[J]. , 2010, 18(5): 607-614.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2010.05.001

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2010/V18/I5/607

References

[1] Guicherit R,Roemer M.Tropospheric ozone trends[J].Chemosphere-Global Science,2000,2(2):167-183
[2] McKenzie R L,Aucamp P J,Bais A F,et al.Changes biologically-active ultraviolet radiation reaching the Earth′s surface[J].Photochemical and Photobiological Sciences,2007,6:218-231
[3] Teramura A H,Sullivan J H,Lydon J.Effects of UV-B radiation on soybean yield and seed quality:a 6-year field study[J].Physiologia Plantarum,1990,80(1):5-11
[4] Caldwell M M,Björn L O,Bornman J F,et al.Effects of increased solar ultraviolet radiation on terrestrial ecosystem[J].Journal of Photochemistry and Photobiology,1998,46(1):40-52
[5] Paul N D,Gwynn-Jones D.Ecological roles of solar UV radiation:towards an integrated approach[J].Trends in Ecology & Evolution,2003,18(1):48-55
[6] Sicora C,Máté Z,Vass I.The interaction of visible and UV-B light during photodamage and repair of Photosystem II[J].Photosynthesis Research,2003,75(2):127-137
[7] Flint S D,Ryel R J,Caldwell M M.Ecosystem UV-B experiments in terrestrial communities:a review of recent findings and methodologies[J].Agricultural and Forest Meteorology,2003,120(3):177-189
[8] 吴学明.青藏高原萹蓄、车前叶绿体超微结构研究[J].西北植物学报,1997,17(4):98-102
[9] 卢存福,简令成,贲桂英.高山植物短管兔儿草光合作用特性及其对冰冻胁迫的反应[J].植物学通报,2000,17(6):559-564
[10] 师生波,贲桂英,韩发.不同海拔地区紫外线B辐射状况及植物叶片紫外线吸收物质含量的分析[J].植物生态学报,1999,23(6):529-535
[11] Bj(o)rn L O,Teramura A H.Simulation of daylight ultraviolet radiation and effects of ozone depiction[A].In:Young AR,Bjrn LO,Moan J,Nultsch W,eds.Environmental UV Photobiology[C].New York:Plenum Press,1993.41-71
[12] Oxborough K,Baker N R.Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components-calculation of qP and F′_v/F′_m without measuring F′_o[J].Photosynthesis Research,1997,54(2):135-142
[13] Genty B,Briantais J M,Baker N R.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochimica et Biophysica Acta,1989,990(1):87-92
[14] Arnon D I.Copper enzymes in isolated chloroplasts:polyphenoloxidase in Beta vulgaris[J].Plant Physiology,1949,24:1-15
[15] 朱广廉.植物生理学实验[M].北京:北京大学出版社,1990.51-54
[16] Joshi P N,Ramaswamy N K,Iyer R K,et al.Partial protection of photosynthetic apparatus from UV-B-induced damage by UV-A radiation[J].Environmental and Experimental Botany,2007,59 (2):166-172
[17] Baker N R.Chlorophyll fluorescence:a probe of photosynthesis in vivo[J].Annual Review of Plant Biology,2008,59:89-113
[18] Allen D J,Nogués S,Baker N R.Ozone depletion and increased UV-B radiation:is there a real threat to photosynthesis ?[J].Journal Experimental Botany,1998,49:1775-1788
[19] Wang G H,Hao Z J,Anken R H,et al.Effects of UV-B radiation on photosynthesis activity of Wolffia arrhiza as probed by chlorophyll fluorescence transients[J].Advances in Space Research,2010,45:839-845
[20] Šprtová M,Špunda V,Kalina J,et al.Photosynthetic UV-B response of beach (Fagus sylvatica L.) saplings[J].Photosynthetica,2003,41(4):533-543
[21] Lizana X C,Hess S,Calderini D F.Crop phenology modifies wheat responses to increased UV-B radiation[J].Agricultural and Forest Meteorology,2009,149(11):1964-1974
[22] Middleton E M,Teramura A H.The role of flavonol glycoside and carotenoids in protecting soybean from ultraviolet-B damage[J].Plant Physiology,1993,103:741-752
[23] Sicora C,Szilárd A,Sass L,et al.UV-B and UV-A radiation effects on photosynthesis at the molecular level[A].In:Ghetti F,Checcucci G,Bornman JF eds.Environmental UV Radiation:Impact and Human Health and Predictive Model[C].Springer,2006.121-135
[24] Jansen M A K,Gaba V,Greenberg B M.Higher plants and UV-B radiation:balancing damage,repair and acclimation[J].Trends in Plant Science,1998,3(4):131-135
[25] Fedina I,Georgieva K,Velitchkova M,et al.Effect of pretreatment of barley seedlings with different salts on the level of UV-B induced and UV-B absorbing compounds[J].Environmental and Experimental Botany,2006,56(3):225-230
[26] Lau T S L,Eno E,Goldstein G,et al.Ambient levels of UV-B in Hawaii combined with nutrient deficiency decrease photosynthesis in near-isogenic maize lines varying in leaf flavonoids:Flavonoids decrease photoinhibition in plants exposed to UV-B[J].Photosynthetica,2006,44(3):394-403
[27] Beggs C G,Wellmann E.Photocontrol of flavonoid biosynthesis[A].In:Kendrick RE,Kronenberg GHM,eds.Photomorphogenesis in Plants.Volume 2[C].Kluwer Academic,Dordrecht,1994.733-750
[28] Ziska L H,Teramura A H,Sullivan J H,et al.Influence of ultraviolet-B (UV-B) radiation on photosynthetic and growth characteristics in field-grown cassava (Manihot esculentum Crantz.)[J].Plant,Cell and Environment,1993,16(1):73-79

Effect of Enhanced UV-B Radiation on Photosynthesis and Photosynthetic Pigments in Alpine Plants Saussurea superba

增补UV-B辐射对高山植物美丽风毛菊光合作用和光合色素的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YAO Xi-xi, WANG Li-ya, YAN Zhen-ying, LI Quan-lin, WANG You-bin, MA Bing-yun, LEI Yan-min, ZHOU Rui, XIE Jiu-xiang. Characteristics of Forage Nutritional Quality and Digestibility in Different Types of Grassland in Qinghai Tibet Plateau and their Correlation [J]. Acta Agrestia Sinica, 2021, 29(S1): 113-120.
[2]	WANG Bao-qiang, ZHAO Ying, ZHU Xiao-lin, WANG Wang-tian, WEI Xiao-hong. Effects of Saline-alkali Stress on Photosynthesis Characteristics and Antioxidant System in Quinoa Seedlings Leaf [J]. Acta Agrestia Sinica, 2021, 29(8): 1689-1696.
[3]	QIN Jin-ping, LIU Ying, MA Yu-shou, WANG Yan-long, LI Shi-xiong. Effects of Rest Grazing on Vegetation Characteristics and Photosynthetic Physiology of Dominant Species in Degraded Alpine Grasslands During Greenup Period [J]. Acta Agrestia Sinica, 2021, 29(5): 1034-1042.
[4]	WEI Xing-xing , WU Jiang-qi , LI Guang, WANG Hai-yan,LIU Shuai-nan, ZHANG Shi-kang, ZHANG Juan. Response of Soil Nitrogen Components to Nitrogen Addition in Wet Meadow in the Tibetan Plateau [J]. Acta Agrestia Sinica, 2021, 29(4): 677-683.
[5]	GUO Yan-hong, PU Xiao-jian, PU Xiao-peng, DU Wen-hua. Change Tendency of the Aboveground Biomass and Nutrition Quality in the Alpine Pastoral Area of Qinghai Tibet [J]. Acta Agrestia Sinica, 2021, 29(4): 734-742.
[6]	ZHANG Li-tian, LIU Wei, LIU De-mei, DONG Rui-zhen, WANG Xiao-li, ZHANG Min, RENZENG Qu-zha, BIANBA Pu-chi, YANG Shi-hai, MA Yu-shou. Biomass Distribution Characteristics of Organs in the Reproductive Growth Period of Pennisetum centrasiaticum on the Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2021, 29(12): 2694-2702.
[7]	LU Yong-xiang, ZHAO Man, CHEN Liang-yin, CHENGQi-ming, YOU Ming-hong, LI Da-xu, CHEN Shi-yong, BAI Shi-qie, LI Ping. Effects of Lactobacillus brucei and formic acid addition on fermentation quality and bacterial community of oat silage of different harvest stages in the Tibetan Plateau [J]. Acta Agrestia Sinica, 2020, 28(6): 1736-1743.
[8]	LI Shuang-ming, LUO Jian-zhang, CHEN Cai-fa, XIANG Zuo-xiang, HU Long-xing. Effects of Primo on Heat Tolerance and Turf Characters in Perennial Ryegrass [J]. Acta Agrestia Sinica, 2020, 28(4): 1006-1014.
[9]	LAI Shuai-bin, PAN Xin-ya, JIAN Chun-xia, LI Junbao, KWAK Sang-Soo, XU Bing-cheng, WANG Zhi. Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco [J]. Acta Agrestia Sinica, 2020, 28(1): 20-30.
[10]	LI Jun, QI Guang-ping, KANG Yan-xia, MA Yan-lin, YIN Min-hua, LI Xiao-min, LI Zhi. Photosynthetic Characteristics,Chlorophyll Fluorescence Parameters and Biomass of Alfalfa under Different Irrigation Treatments [J]. Acta Agrestia Sinica, 2019, 27(6): 1569-1576.
[11]	ZHANG Yan-fen, YANG Xiao-xia, DONG Quan-min, ZHANG Chun-ping, YU Yang, YANG Zeng-zeng, FENG Bin, CHU Hui, WEI Lin-na, ZHANG Xiao-fang. Effects of Mixed Grazing of Yak and Tibetan Sheep on Feed Intake of Grazing Livestock and Plant Compensation Growth [J]. Acta Agrestia Sinica, 2019, 27(6): 1607-1614.
[12]	HUANG Mei, SHANG Zhan-huan. Research Progress on Poisonous Weeds Treatment Technology in Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2019, 27(5): 1107-1116.
[13]	XU Zhe, REN Jian, TIAN Ying, MI Nan. Effects of Exogenous ABA on Photosynthetic Characteristics of Lolium perenne under Drought Stress [J]. Acta Agrestia Sinica, 2019, 27(5): 1243-1249.
[14]	LIU Qian, LIU Bo-wen, WANG Yang, AO Bao, TIAN Pei-xin, WEN Su-yun, ZHANG Xu-hu, MIAO Yan-jun, XU Yue-fei. Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings [J]. Acta Agrestia Sinica, 2019, 27(4): 898-905.
[15]	JIANG Feng-yan, WEI Xiao-ting, KANG Bin-yue, SHAO Xin-qing. Effects of Warming on Alpine Meadow Diversity and Primary Productivity [J]. Acta Agrestia Sinica, 2019, 27(2): 298-305.