Abstract: Seed germination and seedling growth of Neotyphodium endophytE-infected(E+)and endophytE-free(E-)wild barley(Hordeum brevisubulatum)were observed under temperature and simulated drought stress conditions.Results show that germination rate and germination index of E+ seed were significantly higher than those of E-seeds(P<0.05)under 10℃ and 15℃ low temperature stress and 30℃ high temperature stress.Similar results were seen under stimulated drought stress conditions of-0.6 MPa,-0.9 MPa and-1.2 MPa osmotic potential.Moreover,shoot length,root length and seedling water content of E+ seedling were significantly higher than those of E-seedling(P<0.05)under stress conditions at 10℃,15℃,30℃ and-0.3 MPa～-1.2 MPa.This study shows that Neotyphodium endophyte can improve the germination rate,germination speed,seedling growth,capacity of water self-absorption of wild barley under lower and higher temperature and water stress conditions.

Key words: Wild barley, Grass endophyte, Temperature, Drought stress, Germination, Seed

摘要： 本文通过比较携带(E+)和未携带(E-)内生真菌(Neotyphodium)的野大麦种子(Hordeum brevisubulatum)在温度胁迫和模拟干旱胁迫条件下种子的发芽速度、发芽率、根苗长以及幼苗含水量等方面的差异,探讨了内生真菌在对宿主植物抗逆境胁迫条件下的增益作用。结果表明:在10℃,15℃低温胁迫和30℃高温胁迫时,E+种子的发芽率、发芽指数显著大于E-种子(P<0.05);而在模拟干旱胁迫条件下,-0.6~-1.2 MPa时,E+的发芽率和发芽指数显著高于E-(P<0.05)。表明内生真菌可以提高禾草在温度和干旱胁迫下的发芽率和发芽速度。另外,在10℃,15℃低温和30℃高温,及渗透势为-0.3~-1.2 MPa胁迫条件下,E+的胚根长、胚芽长和幼苗含水量也显著大于E-(P<0.05),表明内生真菌可以促进宿主植物在温度和干旱胁迫环境中发芽后幼苗的生长,并增强植株自主吸收水分的能力。

关键词: 野大麦, 内生真菌, 温度, 干旱, 发芽, 种子