Influence of Chlorophyll Fluorescence Characteristics on Alfalfa Seedlings under Cryogenic Stress

doi:10.11733/j.issn.1007-0435.2011.04.010

›› 2011, Vol. 19 ›› Issue (4): 596-600.DOI: 10.11733/j.issn.1007-0435.2011.04.010

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Influence of Chlorophyll Fluorescence Characteristics on Alfalfa Seedlings under Cryogenic Stress

CHEN Shi-ru¹, YU Lin-qing², YI Jin¹, WU Ri-na³, JI Ren-hua³, LIU Shu-na⁴

1. Agronomy College, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia 010019, China;
2. Grassland Research Institute of CAAS, Hohhot, Inner Mongolia 010010, China;
3. Forestry College, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia 010019, China;
4. College of life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia 010021, China

Received:2011-01-17 Revised:2011-04-22 Online:2011-08-15 Published:2011-08-15

低温胁迫对紫花苜蓿叶片叶绿素荧光特性的影响

陈世茹¹, 于林清², 易津¹, 乌日娜³, 吉仁花³, 刘曙娜⁴

1. 内蒙古农业大学农学院, 内蒙古呼和浩特010019;
2. 中国农业科学院草原研究所, 内蒙古呼和浩特010010;
3. 内蒙古农业大学林学院, 内蒙古呼和浩特010019;
4. 内蒙古大学生命科学学院, 内蒙古呼和浩特010021

通讯作者: 于林清,E-mail:linqin_yu@126.com
作者简介:陈世茹(1984- ),女,内蒙占通辽市人,硕士研究生,主要从事牧草生理生态方面的研究,E-mail:lichych@163.com
基金资助:
中央级公益性科研院所基本科研业务费专项资金;国家科技支撑项目(2008BADB3B06)资助

Abstract

Abstract: Chlorophyll fluorescence parameters were measured in alfalfa varieties with different fall dormancy under room temperature and low temperature (-8℃),respectively.The influence of low-temperature on chilling index and the chlorophyll fluorescence parameters were analyzed in different alfalfa varieties.Results show that after cryogenic stress,maximum photochemical efficiency (F_v/F_m) of PS Ⅱ in both Dona Ana and Pierces varieties decreases significantly.Electron transfer rate (ETR) and actual photochemical quantum yield (Y) decreased suggesting that the leaves of both cultivars suffer heavier cold injury.There were small change of fluorescent parameters for Vernal and 5246.The non-photochemical quenches (NPQ) value increases significantly in non-fall dormancy varieties (CUF101 and UC-1465),but other parameters show no significant change.Under light cryogenic stress,non-photochemical quenches (NPQ) value increased suggesting that the dissipation of excess light energy is conducive to protecting photosynthetic apparatus.Therefore fluorescence parameters of alfalfa varieties are closely related with cold resistance.

Key words: Alfalfa, Cryogenic stress, Chlorophyll fluorescence, Chilling injury index

摘要： 以经过抗寒锻炼的盆栽不同抗寒紫花苜蓿(Medicago sativa L.)品种为材料,分别在室温和低温处理后(-8℃)测定不同秋眠型紫花苜蓿品种的叶绿素荧光参数,分析低温对不同苜蓿品种的冷害指数和叶绿素荧光参数的影响。结果表明:在低温胁迫后非秋眠型Dona Ana和Pierce的PSⅡ最大光化学效率(F_v/F_m)显著降低,电子传递速率(ETR)减慢,实际光化学量子产量(Y)下降,叶片受低温胁迫较重;秋眠型Vernal和5246各荧光参数变化不大,而非秋眠型CUF101、极非秋眠型UC-1465非光化学淬灭(NPQ)值显著升高(P<0.05),其他的参数变化较小。低温胁迫很轻时,非光化学淬灭系数NPQ升高,说明耗散过剩的光能具有保护光合机构的作用。因此紫花苜蓿各荧光参数的变化与其抗寒特性密切相关。

关键词: 苜蓿, 低温胁迫, 叶绿素荧光, 冷害指数

CLC Number:

CHEN Shi-ru, YU Lin-qing, YI Jin, WU Ri-na, JI Ren-hua, LIU Shu-na. Influence of Chlorophyll Fluorescence Characteristics on Alfalfa Seedlings under Cryogenic Stress[J]. , 2011, 19(4): 596-600.

陈世茹, 于林清, 易津, 乌日娜, 吉仁花, 刘曙娜. 低温胁迫对紫花苜蓿叶片叶绿素荧光特性的影响[J]. , 2011, 19(4): 596-600.

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Influence of Chlorophyll Fluorescence Characteristics on Alfalfa Seedlings under Cryogenic Stress

低温胁迫对紫花苜蓿叶片叶绿素荧光特性的影响

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