Dynamic Response of Anti-oxidative Defense System to Drought and Rewatering

doi:10.11733/j.issn.1007-0435.2013.02.016

›› 2013, Vol. 21 ›› Issue (2): 316-321.DOI: 10.11733/j.issn.1007-0435.2013.02.016

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Dynamic Response of Anti-oxidative Defense System to Drought and Rewatering

YANG Wen-quan¹, KOU Jian-cun², LEI Zhong-ping¹, GU Mu-yu², CHENG Ji-min³

1. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
3. Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2012-10-08 Revised:2012-12-06 Online:2013-04-15 Published:2013-04-23

小冠花抗氧化保护系统对干旱胁迫及复水的动态响应

杨文权¹, 寇建村², 雷忠萍¹, 顾沐宇², 程积民³

1. 西北农林科技大学生命科学学院, 陕西杨凌 712100;
2. 西北农林科技大学动物科技学院, 陕西杨凌 712100;
3. 中国科学院水利部水土保持研究所, 陕西杨陵 712100

通讯作者: 寇建村,jiancun02@163.com
作者简介:杨文权(1975-),男,陕西扶风人,博士,讲师,主要从事植物学方面的教学和科研工作,E-mail:wenquan05@sina.com
基金资助:
现代农业产业技术体系建设专项资金(CARS-35-40);农业部牧草种质资源保护项目 (NB08-2130135-43);西北农林科技大学博士科研启动经费(22050205)资助

Abstract

Abstract: The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), as well as the contents of relative water (RWC), scorbic acid (AsA) and superoxide anion (O₂^-·) of the Coronilla varia leaves were studied during water stress and subsequent rewatering using pot trials. Samples were collected on the 3^rd, 6^th, 9^th, 12^th, 15^th, 18^th day after water stress and the 3^rd day after the rewatering. The plants with 70%～75% soil relative water content were controls. Results showed that RWC, the activities of SOD, POD and CAT within 6 days water stress and APX activity, the contents of AsA and O₂^-· within 9 days water stress did not have significant differences compared with controls. As water stress continued, RWC linearly decreased to 49% on the 12^th day then slowly decreased. The SOD activity presented increase (9 d)-decrease (12～15 d)-increase (18 d) trend. The POD activity increased on 9^th day, then decreased. The CAT activity and the O₂^-· content increased. The APX activity and the AsA content both followed increase (12～15 d)-decrease (18 d) process. After the rewatering, all indexes were back to control level. In conclusion, water stress influenced anti-oxidative defense system and the effects depended on stress time. Coronilla varia has a good adaptability to water stress as system functions can recover soon after rewatering.

Key words: Coronilla varia, Drought, Rewatering, Anti-oxidative defense system

摘要： 采用盆栽控水法对小冠花(Coronilla varia)持续干旱胁迫18 d后复水,以土壤相对含水量保持在70%～75%的小冠花为对照,在持续控水干旱胁迫第3,6,9,12,15,18 d及复水后第3 d分别采集小冠花叶片,研究叶片相对含水量(RWC)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性及抗坏血酸(AsA)、超氧阴离子(O₂^-·)含量对干旱及复水处理的动态响应。结果表明:小冠花叶片RWC及SOD,POD,CAT活性在干旱处理的前6 d内,APX活性、AsA和O₂^-·含量在干旱处理的前9 d内,均和对照无显著差异;此后,叶片RWC直线下降,在干旱处理第12 d时,降至49%,而后又缓慢下降;SOD活性呈上升(9 d)-下降(12～15 d)-上升(18 d)的趋势;POD活性先升(9 d)后降(12～18 d);CAT活性和O₂^-·含量增加;APX活性和AsA含量变化一致,呈先升(12～15 d)后降(18 d)的趋势。但复水后,各指标均能很快恢复至对照水平。说明干旱胁迫对小冠花抗氧化保护系统有重要影响,但此作用和干旱进程密切相关;同时,小冠花对干旱胁迫适应性较强,复水后能很快恢复至正常水平。

关键词: 小冠花, 干旱, 复水, 抗氧化保护系统

CLC Number:

Q948

YANG Wen-quan, KOU Jian-cun, LEI Zhong-ping, GU Mu-yu, CHENG Ji-min. Dynamic Response of Anti-oxidative Defense System to Drought and Rewatering[J]. , 2013, 21(2): 316-321.

杨文权, 寇建村, 雷忠萍, 顾沐宇, 程积民. 小冠花抗氧化保护系统对干旱胁迫及复水的动态响应[J]. , 2013, 21(2): 316-321.

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Dynamic Response of Anti-oxidative Defense System to Drought and Rewatering

小冠花抗氧化保护系统对干旱胁迫及复水的动态响应

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