Screening and Identification of Chitinolytic Actinomycetes and the Inhibitory Activity on Turfgrass Root Rot Disease Fungi

doi:10.11733/j.issn.1007-0435.2013.05.025

Acta Agrestia Sinica ›› 2013, Vol. 21 ›› Issue (5): 998-1004.DOI: 10.11733/j.issn.1007-0435.2013.05.025

Previous Articles Next Articles

Screening and Identification of Chitinolytic Actinomycetes and the Inhibitory Activity on Turfgrass Root Rot Disease Fungi

WANG Qi¹, DUAN Bi-hua², YANG Rui², ZHAO Yao-rong¹, ZHANG Lu¹

1. College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China;
2. College of Plant Science and Technology, Beijing University of Agriculture, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing 102206, China

Received:2013-03-07 Revised:2013-04-19 Online:2013-10-15 Published:2013-10-30

产几丁质酶放线菌的筛选、鉴定及其对草坪草根腐病真菌的抑制作用

王歧¹, 段碧华², 杨瑞², 赵耀荣¹, 张露¹

1. 北京农学院园林学院, 北京 102206;
2. 北京农学院植物科学技术学院农业应用新技术北京市重点实验室, 北京 102206

通讯作者: 段碧华
作者简介:王歧(1987- ),男,吉林磐石人,硕士研究生,研究方向为生物防治, E-mail: leibiyijue@126.com
基金资助:
国家自然科学基金(31000551)(3097012)资助

Abstract

Abstract: Chitinolytic actinomycetes from lawn soil were screened and identified and their inhibitory activities on four types of turfgrass root rot disease were studied. Results showed that screened Bn035 strain had stronger chitinase activity than the other four strains; the relative diameter of chitin-lytic zone was 3.60 and 3.42, respectively. Its chitinase activity in culture broth reached a maximum value of 1.23 U·mL^-1 on the 6^th day. Crude enzyme solution and thallus of Bn035 strain showed stronger antifungal activity against all tested fungi. The Bn035 strain was identified as Streptomyces scabrisporus by morphological, cultural, physiological, biochemical and phylogenetic characteristics. The Bn035 strain had strong chitinase activity and inhibitory activity that suggested its potential application in the biological control of turfgrass fungal root rot disease.

Key words: Chitinase, Actinomycetes, Turfgrass, Root rot disease, Screening, Identification, Inhibition

摘要： 通过对草坪土壤中产几丁质酶放线菌的筛选、鉴定及对4种草坪草根腐病菌的抑制作用研究,为草坪草根腐病的生物防治提供指导。通过菌落法和菌饼法筛选得到1株产几丁质酶活性较强的放线菌菌株Bn035,其降解圈相对直径分别为3.60和3.42;采用DNS法测量几丁质酶活性发现,第6 d时酶活达到最大为1.23 U·mL^-1;菌体和粗酶液均对草坪草根腐病菌产生较强的抑制作用;根据形态学特征、培养特征、生理生化特征和16S rDNA序列比对综合分析,菌株Bn035为皱孢链霉菌(Streptomyces scabrisporus)。菌株Bn035产几丁质酶活性较强且抑菌效果较好,表明其在真菌性根腐病生物防治方面具有潜力。

关键词: 几丁质酶, 放线菌, 草坪草, 根腐病, 筛选, 鉴定, 抑制作用

CLC Number:

S154.39

WANG Qi, DUAN Bi-hua, YANG Rui, ZHAO Yao-rong, ZHANG Lu. Screening and Identification of Chitinolytic Actinomycetes and the Inhibitory Activity on Turfgrass Root Rot Disease Fungi[J]. Acta Agrestia Sinica, 2013, 21(5): 998-1004.

王歧, 段碧华, 杨瑞, 赵耀荣, 张露. 产几丁质酶放线菌的筛选、鉴定及其对草坪草根腐病真菌的抑制作用[J]. 草地学报, 2013, 21(5): 998-1004.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2013.05.025

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2013/V21/I5/998

References

[1] 熊晶. 产几丁质酶菌株的筛选与鉴定及几丁质酶酶学性质研究. 长沙:中南大学,2009:13

[2] Velusamy P,Kim K Y. Chitinolytic activity of Enterobacter sp. KB3 antagonistic to Rhizoctonia solani and its role in the degradation of living fungal hyphae[J]. International Research Journal of Microbiology,2011,2(6):206-214

[3] 檀建新,陈忠义,张杰,等.产几丁质酶菌的分离鉴定及其抑菌作用的初步研究[J]. 植物保护,2001,27(2):1-3

[4] Zarei M,Aminzadeh S,Zolgharnein H,et al. Characterization of a chitinase with antifungal activity from a native Serratla marcescens B4A[J]. Brazilian Journal of Microbiology,2011,42(3):1017-1029

[5] 石洁,李建成,刘玉瑛,等. 草坪草根腐病病原菌研究初报[J]. 华北农学报,2000,15(S1):94-98

[6] 孙炳剑,袁红霞,邢小萍,等. 郑州地区冷季型草坪草根腐病病原鉴定[J]. 草原与草坪,2007(6):51-54

[7] 张培志,何田,吴瑛. 杀菌剂三唑酮在我国农业中的应用研究进展[J]. 浙江科技学院学报,2004,16(1):28-34

[8] 季顺卿,段青芳,徐国超,等. 白叶草坪菌核性根腐病防治方法探讨[J]. 平顶山工学院学报,2006,15(1):48-49

[9] 冯轶男,杨润清. 放线菌分离与筛选方法的研究进展[J]. 生物技术,2010,20(4):95-98

[10] 赵娟,薛泉宏,王玲娜,等. 多功能放线菌Act12 对土传病原真菌的拮抗性及其鉴定[J]. 中国生态农业学报,2011,19(2):394-398

[11] Narayana K,Vijayalakshm M. Chitinase production by Streptomyces sp. ANU 6277[J]. Brazilian Journal of Microbiology,2009,40(4):725-733

[12] 高小宁,古丽皮艳,王美英,等. 产几丁质酶内生放线菌的筛选及其对核盘菌的抑制作用[J]. 浙江大学学报:农业与生命科学版,2010,36(6):615-622

[13] 杨海英,杜刚,鲁开凤[J]. 抑制剂对土壤放线菌分离的影响[J]. 湖北农业科学,2008,47(4):435-437

[14] 赵凯,许鹏举,谷广烨. 3, 5-二硝基水杨酸比色法测定还原糖含量的研究[J]. 食品科学,2008,29(8):534-536

[15] 方中达. 植病研究方法[M]. 北京:中国农业出版社,2007:246-249

[16] 阮继生,黄英. 放线菌快速鉴定与分类系统[M]. 北京:科学出版社,2011:69-71

[17] 王平. 测定放线菌菌体中氨基酸和单糖的快速方法-薄层层析法[J]. 微生物学报,1986,13(5):228-230

[18] 徐丽华,李文均,刘志恒,等. 放线菌系统学原理、方法及实践[M]. 北京:科学出版社,2007:40-45

[19] 徐平,李文均,徐丽华. 微波法快速提取放线菌基因组DNA[J]. 微生物学通报,2003,3(4):82-85

[20] 丁湖广.菌种退化与老化原因及防止措施[J]. 特种经济动植物,2006,9(1):39-40

[21] 奚家勤,宋纪真,周汉平. 产几丁质酶菌的分离、筛选及其对烟草病原真菌的抑制作用[J]. 烟草科技,2006(3):55-58

[22] 岳会敏,杨谦,宋金柱. 两种毛壳茵对几种植物病原菌的生防效果分析[J]. 哈尔滨商业大学学报:自然科学版,2009,25(5):593-596

[23] Xu P,Takahashi Y K,Seino A,et al. Streptomyces scabrisporus sp. Nov[J]. International Journal of Systematic and Evolutionary Microbiology,2004,54(4):577-581

Screening and Identification of Chitinolytic Actinomycetes and the Inhibitory Activity on Turfgrass Root Rot Disease Fungi

产几丁质酶放线菌的筛选、鉴定及其对草坪草根腐病真菌的抑制作用

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Li-hui, LIU Yi-ling, ZHAO Wen-xing, ZHANG Yun-hao, WU Ke-xin, GUO Zi-yu, MO Chong-hui, ZHAO Bao-yu, LU Hao. Isolation,Identification and Tissue Distribution Characteristics of Fungal Endophyte in Ligularia sagitta [J]. Acta Agrestia Sinica, 2021, 29(8): 1658-1665.
[2]	LIU Bo-wen, LI Gui-yang, CHANG Yuan-fei, JIN Mei-yan, LIU Fang, GAO Qiu, LIU Wan-liang, Hasitamier, WANG Xian-guo. Study on Seed Morphological Diversity and Seed Classification and Identification Method of Vicia [J]. Acta Agrestia Sinica, 2021, 29(7): 1375-1385.
[3]	YE Gang, ZHANG Xiao-xiao, CHEN Jing-bo, LI Jian-jian, LI Ling, LIU Jian-xiu, GUO Ai-gui, GUO Hai-lin. Morphological Characteristics and Identification of SRAP Molecular Markers in Hybrid Progeny of Zoysia [J]. Acta Agrestia Sinica, 2021, 29(7): 1406-1415.
[4]	YANG Wan-qiu, JING Jie, ZHU Ling, GAO Yong-heng. Screening and Characteristics of Plant Growth-Promoting Rhizobacteria From Alpine Meadow Plants in Northwestern Sichuan [J]. Acta Agrestia Sinica, 2021, 29(6): 1174-1182.
[5]	XING Hui-qin, WANG Chun-ming, JIN She-lin, ZHOU Tian-wang, GUO Cheng. Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot [J]. Acta Agrestia Sinica, 2021, 29(6): 1350-1356.
[6]	TONG Yong-shang, YU Xiao-jun, XU Chang-lin, WANG Peng-bin, SONG Jian-chao, LI Zhen, LI Ying. Effects of Sowing Date on Forage Yield and Quality of Seven Oat Varieties in Tianzhu Alpine Region [J]. Acta Agrestia Sinica, 2021, 29(5): 1094-1106.
[7]	ZHANG Xiao-jie, WANG Chun-ming, ZHOU Tian-wang, LIU Hao, SHEN Rui-qing, LI Min-quan, GUO Cheng. Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia [J]. Acta Agrestia Sinica, 2021, 29(4): 805-812.
[8]	WANG Jing, FU Bing-zhe. Research Progress on Chromosome Doubling of Grass Based on Bibliometric Analysis [J]. Acta Agrestia Sinica, 2021, 29(3): 413-424.
[9]	XU Zheng, XU Qian, LIU Ming-xi, XIANG Zuo-xiang. Effect of ‘Henace’ Herbicide on the Spring Transition of Zoysia japonica ‘Lantai No.3’ Overseeding Turfgrass [J]. Acta Agrestia Sinica, 2021, 29(3): 603-609.
[10]	WEI Xing-di, ZENG Qing-fei, WEI Xin, LI Ya-jiao, OU Er-ling, LIU Zheng-shu. The Rhizobia and High Efficient Strains Screening of Indigofera amblyantha in Karst Mountain Region of Guizhou [J]. Acta Agrestia Sinica, 2021, 29(11): 2413-2421.
[11]	YANG Qing, WANG Yi-xiang, LI Xin-xin, WENG Bo-qi, LIAO Hong. Isolation and Screening of Effective Rhizobial Strains of Cassia (Chamaecrista spp.) Pasture in Acidic Soils [J]. Acta Agrestia Sinica, 2020, 28(6): 1519-1526.
[12]	SUN Hao-yang, ZHAO Gui-qin, ZHANG Jian-gui, LI Xin, YANG Yan-shan, CHAI Ji-kuan, ZENG Liang, JIAO Run-an, JIN Xiao-wen, LI Rong, GONG Wen-long. Incidence of Oat Powdery Mildew and Pathogen Identification in the Main Oat Production Areas of Gansu Province [J]. Acta Agrestia Sinica, 2020, 28(6): 1535-1543.
[13]	LI Yu-ying, LIU Na, JING Ze-yao, WEN Wei, CAO Lei, XIA Fang-shan, ZHU hui-sen. Effect of Extract from Artemisia annua on Seed Germination and Seedling Growth of Four Species of Turfgrass [J]. Acta Agrestia Sinica, 2020, 28(5): 1285-1293.
[14]	WU Rui-xin, Li Yuan, YOU Yong-liang, LIU Gui-bo, ZHAO Hai-ming. Study on Drought Resistance Identification and Evaluation Methods of Alfalfa during Whole Growth Period [J]. Acta Agrestia Sinica, 2020, 28(5): 1444-1453.
[15]	CUI Hui-ting, SUN Xi-nuo, MA Cheng-ze, HU Qian-nan, SUN Yan. Advances in Applications of Metabolomics Technology in Stress Resistance of Forage and Turfgrass [J]. Acta Agrestia Sinica, 2020, 28(4): 873-880.