Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (3): 542-549.DOI: 10.11733/j.issn.1007-0435.2014.03.016

Previous Articles     Next Articles

Dynamic Responses of UV-absorbing Compounds and Osmotic Adjustment Substances of Lespedeza davurica to Drought and Enhanced UV-B Radiation

CAI Cai-hong1, TIAN Feng2, DU Run-feng3, YANG Tu-xi4, HAO Wen-fang1   

  1. 1. School of Life Science, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    2. School of Chemistry and Chemical Engineering, Southwest University, Chonging 400715, China;
    3. Guangdong Testing Institute for Product Quality, Guangzhou, Guangdong Province 528300, China;
    4. School of Forestry, Northwest A&F University, Yangling, Shaanxi Province 712100, China
  • Received:2013-09-09 Revised:2014-02-14 Online:2014-06-15 Published:2014-06-04

达乌里胡枝子紫外吸收物质和渗透调节物质对干旱及增强UV-B辐射的动态响应

蔡彩虹1, 田丰2, 杜润峰3, 杨途熙4, 郝文芳1   

  1. 1. 西北农林科技大学生命科学学院, 陕西 杨凌 712100;
    2. 西南大学化学化工学院, 重庆 400715;
    3. 广东产品质量监督检验研究院, 广东 广州 528300;
    4. 西北农林科技大学林学院, 陕西 杨陵 712100
  • 通讯作者: 郝文芳
  • 作者简介:蔡彩虹（1988- ），女，河南安阳人，硕士研究生，研究方向为植物抗旱生理，E-mail：caicaihong163@126.com
  • 基金资助:

    林业公益性行业科研专项（201304312）；中国科学院“西部之光”人才培养项目（2008DF02）资助

PDF

Abstract:

The effects of different moisture intensities (75%±5%, 55%±5%, 35%±5% of field capacity) combined with enhanced UV-B radiation (3.002 kJ·m-2·d-1) on UV-absorbing compounds and osmotic adjustment substances in the leaves of potted Lespedeza davurica were investigated under controlled conditions. The results showed that the flavonoid contents of two treatments increased 39.2% (T1) and 44.4% (T2) under drought stress compared with well-watered group. Total hydroxybenzene contents increased with the increase of drought stress. The activity of phenylalanine ammonia-lyase increased first and then decreased under mild drought stress. The contents of flavonoid and total hydroxybenzene increased and the activity of phenylalanine ammonia-lyase was stimulated at the same time under combined treatment, while there were significant differences between combined treatment groups. In addition, the accumulations of osmotic adjustment substances had a big difference under different drought stress intensities and periods. The content of free proline increased during the early stage of drought stress, while the content of sugar reached a significant level during the medium stage of drought stress, and the content of soluble sugar highly accumulated during the later stage of severe drought stress. The content of free proline increased first and then decreased under the combined treatment of well-watered and enhanced UV-B radiation, but decreased under the combined treatment of drought stress and enhanced UV-B radiation. Soluble protein synthesis was inhibited under the combined treatment of well-watered and enhanced UV-B radiation, but induced under the drought combined treatment. Soluble sugar content decreased under the combined treatment, but increased with the decrease of moisture. According to subordinate function analysis, the antioxidant capacity of Lespedeza davurica was enhanced as the drought intensity was elevated, By contrast with the effect of combined treatment of well-watered and enhanced UV-B radiation on antioxidant capacity, Lespedeza davurica had higher antioxidant capacity under the combined treatment of mild drought stress and enhanced UV-B radiation.

Key words: Lespedeza davurica, Enhanced UV-B radiation, Drought stress, UV-absorbing compounds, Osmotic adjustment substances

摘要：

采用盆栽试验研究达乌里胡枝子（Lespedezas davurica）在不同水分梯度（适宜水分、轻度干旱胁迫、重度干旱胁迫），及不同水分梯度和增强UV-B辐射复合处理下其叶片中紫外吸收物质和渗透调节物质的动态响应。结果表明：类黄酮含量在轻度和重度干旱胁迫下分别较适宜水分下升高39.2%和44.4%，总酚含量在重度胁迫下极显著升高（P<0.01），苯丙氨酸解氨酶（PAL）活性在轻度胁迫下先升高后降低。复合处理下类黄酮、总酚含量及PAL活性升高，各处理间差异显著。干旱胁迫下，游离脯氨酸含量在胁迫前期大量增加，可溶性蛋白含量在胁迫中期极显著升高，可溶性糖在重度干旱胁迫后期大量积累。UV-B辐射对不同水分梯度下三者含量影响不同：游离脯氨酸含量在适宜水分和增强UV-B辐射复合处理下升高，在干旱和增强UV-B辐射复合处理下降低；可溶性蛋白合成在适宜水分和增强UV-B辐射复合处理初期受到一定的抑制，在干旱和增强UV-B辐射复合处理下被诱导；可溶性糖在复合处理下含量下降，但随着水分减少其下降幅度有所减弱。隶属函数综合分析表明，干旱胁迫下达乌里胡枝子抗氧化能力随着干旱胁迫强度增加而增加，增强UV-B辐射下轻度干旱胁迫使其抗氧化能力增强，而重度干旱胁迫使其抗氧化能力降低。

关键词: 达乌里胡枝子, 增强UV-B辐射, 干旱胁迫, 紫外吸收物质, 渗透调节物质

CLC Number: