Abstract:

Peroxidase (POD) is ozymes of parent and hybrids of reciprocal cross combination were analyzed by PAGE in order to study their genetic variation in red soil in Yunnan province. The result showed that the 10 enzyme bands of POD isozymes were separated from ‘Deqin’♀ and‘Acrora’♂ as parent and their F₁, F₂ hybrids. The largest genetic distance was 0.60 among F₂ hybrids. The 8 enzyme bands of POD isozymes were separated from ‘Deqin’♂ and ‘Acrora’♀ as parent and their F₁, F₂ hybrids. The largest genetic distance was 0.50 among F₂ hybrids. The variation among the F₂ hybrids of Deqin ♀ as parent was bigger than that of ‘Deqin’♂ and ‘Acrora’♀ as parent. So the peroxidase isozyme features of alfalfa hybrids were influenced by different genetic backgrounds.

Key words: Medicago sativa L., Peroxidase isozyme, Genetic variation

摘要：

为明确云南红壤区紫花苜蓿(Medicago sativa)亲本及其杂种后代的遗传变异,采用了不连续聚丙烯酰胺凝胶电泳技术,对分别以‘德钦’苜蓿与‘Acrora’为父母亲本的正反杂交组合的亲本、F₁代和F₂代过氧化物同工酶进行分析.结果表明:‘德钦’苜蓿♀与‘Acrora’♂杂交的同工酶谱带有10条,F₂代之间的遗传距离最大为0.60;‘德钦’苜蓿♂与‘Acrora’♀杂交的同工酶谱带有8条,F₂代之间的遗传距离最大为0.50.以‘德钦’苜蓿为母本F₂代之间的变异比以‘德钦’苜蓿为父本F₂代之间的变异大.不同遗传背景对紫花苜蓿后代POD同工酶酶谱特征的影响较大.

关键词: 紫花苜蓿, 过氧化物同工酶, 遗传变异