Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L.

doi:10.11733/j.issn.1007-0435.2015.03.010

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (3): 496-501.DOI: 10.11733/j.issn.1007-0435.2015.03.010

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Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L.

MA Wen-bin^1,2, YAO Tuo^1,2,3, RONG Liang-yan^1,2, WANG Guo-ji^1,2, SUN Guang-zheng^1,2, LIU Huan^1,2

1. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu Province 730070, China;
3. Sino-U.S.Centers for Sustainable Development of Grassland and Animal Husbandry, Lanzhou, Gansu Province 730070, China

Received:2014-05-06 Revised:2014-12-16 Online:2015-06-15 Published:2015-07-02

无外源氮素条件下接种促生菌对箭筈豌豆生长及根系特性影响

马文彬^1,2, 姚拓^1,2,3, 荣良燕^1,2, 王国基^1,2, 孙广正^1,2, 刘欢^1,2

1. 甘肃农业大学草业学院草业科学实验室, 甘肃兰州 730070;
2. 草业生态系统教育部重点实验室, 甘肃兰州 730070;
3. 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 姚拓
作者简介:马文彬 (1989-),女,甘肃白银人,硕士研究生,研究方向为草地微生物资源与生态,E-mail :843606723@qq.com
基金资助:
国家自然基金(31360584);现代农业产业技术体系(CARS-35) ;甘肃省农业科技创新项目(GNCX-2012-45)资助

Abstract

Abstract:

Three PGPR(Strain No.: G3, Y16, J3-1) strains were isolated and screened from the rhizosphere of vetch(Vicia sativa). Both single inoculum and compound inoculum were designed with these strains. Effect of inoculum on the growth and root system characteristics of vetch was determined by an indoor experiment. The results indicated that the plant height, biomass, diameter, root length and root activity of vetch increased after using inoculants. The effectiveness of treatment D (phosphate dissolving + nitrogen-fixing+rhizobia inoculants :G3+Y16+J3-1) was better than single inoculant. The plant height, aboveground biomass, underground biomass, root length, surface area, root volume, root diameter and root activity increased by 45.75%, 177.16%, 211.69%, 73.7%, 238.9%, 199.2%, 12.4% and 44.2%, respectively. Meanwhile, A(G3) was the best single inoculum. The compound inoculum (G3+Y16+J3-1) was recommended as the best resources to substitute rhizosphere inoculum for Vicia sativa.

Key words: Vicia sativa L., Plant growth promoting rhizobacteria (PGPR), Inoculum, Biomass, Root system characteristics

摘要：

利用前期筛选的优良促生菌株研制微生物接种剂,在无外源氮素条件下测定其对箭筈豌豆(Vicia sativa)生长及根系特性的影响。结果表明:无外源氮素条件下,接种研制的优良促生菌接种剂对箭筈豌豆地上及根系生长有显著促进作用,箭筈豌豆株高、生物量、部分根系形态学指标(根长、根表面积、根体积及根平均直径)以及根系活力等均显著提高。不同处理中,复合接种剂效果优于单一接种剂。复合接种剂中,处理D(根瘤菌+联合固氮菌+溶磷菌:G3+Y16+J3-1)效果最佳,其对箭筈豌豆株高、地上生物量、地下生物量、根长、根表面积、根体积、根平均直径及根系活力分别较对照增加45.75%,177.16%,211.69%,73.7%,238.9%, 199.2%,12.4%和44.2%。虽然单一根瘤菌剂(G3)表现出与D处理相近的促生效果, 但D处理对根系活力提高显著,所以推荐根瘤菌+联合固氮菌+溶磷菌株的复合接种剂作为后期研制箭筈豌豆生物菌肥的最佳菌株组合。

关键词: 箭筈豌豆, 促生菌, 接种剂, 生物量, 根系特性

CLC Number:

S551.9

MA Wen-bin, YAO Tuo, RONG Liang-yan, WANG Guo-ji, SUN Guang-zheng, LIU Huan. Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L.[J]. Acta Agrestia Sinica, 2015, 23(3): 496-501.

马文彬, 姚拓, 荣良燕, 王国基, 孙广正, 刘欢. 无外源氮素条件下接种促生菌对箭筈豌豆生长及根系特性影响[J]. 草地学报, 2015, 23(3): 496-501.

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Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L.

无外源氮素条件下接种促生菌对箭筈豌豆生长及根系特性影响

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