Characterization and Identification of High Quality Lactic Acid Bacteria from Hulless Barley Straw Silage

doi:10.11733/j.issn.1007-0435.2015.03.025

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (3): 607-615.DOI: 10.11733/j.issn.1007-0435.2015.03.025

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Characterization and Identification of High Quality Lactic Acid Bacteria from Hulless Barley Straw Silage

CUI Zhao-ming¹, GUO Gang^1,2, YUAN Xian-jun¹, LI Jun-feng¹, YANG Xiao-dan¹, DING Liang¹, YU Cheng-qun³, SHAO Tao¹

1. Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
3. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China

Received:2014-07-14 Revised:2014-12-03 Online:2015-06-15 Published:2015-07-02

青稞秸秆青贮饲料中优良乳酸菌的筛选及鉴定

崔棹茗¹, 郭刚^1,2, 原现军¹, 李君风¹, 杨晓丹¹, 丁良¹, 余成群³, 邵涛¹

1. 南京农业大学饲草调制加工与贮藏研究所, 江苏南京 210095;
2. 山西农业大学动物科学技术学院, 山西太谷 030801;
3. 中国科学院地理科学与资源研究所, 北京 100101

通讯作者: 邵涛
作者简介:崔棹茗(1990-),女,吉林公主岭人,硕士,主要从事饲草调制加工研究,E-mail: cuizhaoming096@163.com
基金资助:
中国科学院科技服务网络计划(STS计划)“西藏典型农业村(吉纳村)草地农业体系建设与产业化示范”(KFJ-EW-STS-071);国家星火计划项目课题“优质饲草种植与奶牛健康养殖技术集成与示范”(2013GA840003);“十二五”国家科技支撑计划“藏北退化草地综合整治技术与示范”(2011BAC09B03)资助

Abstract

Abstract:

The objectives of this study were to select the suitable lactic acid bacteria of highland barley straw silage and research the new type of silage additives in Tibetan Plateau. Optimal lactic acid bacteria were isolated and screened from hulless barley straw silage in Tibetan Plateau. Conventional microbial identification and 16S rRNA sequence analysis methods were used to identify these lactic acid bacteria. A total number of four homo-fermentative lactic acid bacteria strains were isolated, of which HG24 and LH2 were identified as Lactobacillus.Plantarum, LH9 as Pediococcusacidi lactici and LH7 as Pediococcus pentosaceus. All isolated strains could grow at 5～30℃, pH 3.5～7.0 and 3.0% ～ 6.5% NaCl. In addition, HG24 and LH2 gave a positive react at pH 3.0. The characteristics of lactic acid bacteria isolated in Tibetan Plateau included low temperature, high acid and high salt resistances. Of all the selected strains, HG24 and LH2 were the eximious strains to be used as silage additive with the unique abilities of high acid production and fast growth.

Key words: Hulless barley straw, Silage, Lactic acid bacteria

摘要：

为筛选出适合于青藏高原青稞秸秆青贮用的优良乳酸菌菌株,研发新型青贮添加剂。分离了青藏高原青稞(Hordeum vulgare)秸秆青贮饲料中的乳酸菌,利用传统微生物培养法和16S rRNA序列分析法对乳酸菌进行鉴定,探讨其生物学特性,并筛选出优良的乳酸菌菌株。共分离获得4株同型发酵乳酸菌菌株,其中HG24,LH2为植物乳杆菌(Lactobacillusp lantarum),LH9为乳酸片球菌(Pediococcusacidi lactici),LH7为戊糖片球菌(Pediococcusp entosaceus)。分离出的乳酸菌菌株均能在5～30℃,pH 3.5～7.0及3.0%和6.5% NaCl培养基中生长良好,其中HG24,LH2在pH值为3的培养基中仍能生长,体现出分离的乳酸菌菌株具有耐低温、耐酸性和耐盐性强的特征。4株乳酸菌菌株中HG24和LH2产酸能力强、生长速率快,最适宜于用作青贮乳酸菌菌种。

关键词: 青稞秸秆, 青贮饲料, 乳酸菌

CLC Number:

S816.53

CUI Zhao-ming, GUO Gang, YUAN Xian-jun, LI Jun-feng, YANG Xiao-dan, DING Liang, YU Cheng-qun, SHAO Tao. Characterization and Identification of High Quality Lactic Acid Bacteria from Hulless Barley Straw Silage[J]. Acta Agrestia Sinica, 2015, 23(3): 607-615.

崔棹茗, 郭刚, 原现军, 李君风, 杨晓丹, 丁良, 余成群, 邵涛. 青稞秸秆青贮饲料中优良乳酸菌的筛选及鉴定[J]. 草地学报, 2015, 23(3): 607-615.

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Characterization and Identification of High Quality Lactic Acid Bacteria from Hulless Barley Straw Silage

青稞秸秆青贮饲料中优良乳酸菌的筛选及鉴定

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