Abstract:

To optimize the condition of EMS mutagenesis of burclover (Medicago polymorpha L.) seeds, obtain mutation lines with stable behavior,we used Huaiyang burclover seed as plant material, and treated it with Ethyl methyl sulfonate (EMS), Five ethyl methane sulfonate (EMS) concentrations (0.1%, 0.3%, 0.5%, 0.7% and 0.9%), and 3 processing time (3 h, 6 h and 9 h) were used for mutagenesis. The germination rate and radicle length, transplanting survival rate and relative survival rate were analyzed. The results showed that EMS mutagenesis time and concentration of the Huaiyang burclover germination rate and radicle length had an inhibitive effect, and the inhibition effect of the concentration of EMS greater than the treatment time; EMS treatment had little effect on the survival rate of transplanting Huaiyang burclove; under low concentration of EMS treatment, Huaiyang burclover maintained higher survival rate; high concentration of EMS inhibited burclover overwintering. The treatment of 0.7% EMS for 9 h and 0.9% EMS for 6 h got a seed germination rate closed to 50%, and these two treatments were optimized for burclover seed mutatigenesis.

Key words: Ethyl methyl sulfonate (EMS), Medicago polymorpha, Chemical mutagenesis, Mutant, Semi lethal conditions

摘要：

为确定EMS诱变处理金花菜（Medicago polymorpha L.）种子的最适条件，获得化学诱变性状稳定的突变系和育种后代。本试验以淮扬金花菜种子为材料，EMS为诱变剂，采用5个甲基磺酸乙酯（EMS）诱变浓度（0.1%，0.3%，0.5%，0.7%和0.9%），3个处理时间（3 h，6 h和9 h），分别对诱变后种子发芽率、胚根长度、移栽成活率及相对越冬率进行分析。研究发现EMS诱变处理对淮扬金花菜发芽率和胚根伸长有抑制效应，且EMS诱变浓度抑制效应大于诱变时间；EMS处理对淮扬金花菜移栽成活率影响较小；低浓度EMS处理后淮扬金花菜保持了较高的越冬率，但高浓度EMS抑制金花菜的越冬。0.7% EMS 9 h组合和0.9% EMS 6 h组合的发芽率均接近50%，最终确定0.7% EMS诱变处理9 h和0.9% EMS诱变处理6 h的组合为金花菜种子最佳诱变条件。

关键词: 甲基磺酸乙酯(EMS), 金花菜, 化学诱变, 突变体, 半致死条件