Quantitative Analysis of Herbage Nutritional Components on Natural Pasture by Near Infrared Diffuse Reflectance Spectroscopy

doi:10.11733/j.issn.1007-0435.2018.01.032

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (1): 249-255.DOI: 10.11733/j.issn.1007-0435.2018.01.032

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Quantitative Analysis of Herbage Nutritional Components on Natural Pasture by Near Infrared Diffuse Reflectance Spectroscopy

LIU Zhe¹, WANG Yu-qin¹, XUE Shu-yuan², JIN Hai², LI Yuan-xiao¹, WANG Jian-ping¹, LI Fa-di³, ZHANG Pan-pan⁴

1. School of Animal Science and Technology, Henan University of Science and Technology, LuoYang, Henan Provience 471023, China;
2. Inner Mongolian Academy of Agriculture and Animal Science, Huhhot, Inner Mongolian Provience 010031, China;
3. College of Pastoral Agriculture Science and Technology, Lanzhou, Lanzhou, Gansu Provience 730000, China;
4. College of Animal Science, Inner Mongolian Agriculture University, Huhhot, Inner Mongolian Provience 010018, China

Received:2017-05-22 Revised:2018-03-22 Online:2018-02-15 Published:2018-05-25

近红外漫反射光谱定量分析天然牧草营养成分

刘哲¹, 王玉琴¹, 薛树媛², 金海², 李元晓¹, 王建平¹, 李发弟³, 张盼盼⁴

1. 河南科技大学动物科技学院, 河南洛阳 471023;
2. 内蒙古自治区农牧业科学院, 内蒙古呼和浩特 010031;
3. 兰州大学草地农业科技学院, 甘肃兰州 730000;
4. 内蒙古农业大学动物科学学院, 内蒙古呼和浩特 010018

通讯作者: 王玉琴
作者简介:刘哲(1986-),女,河南南阳人,硕士研究生,E-mail:nmgnms@163.com
基金资助:
国家现代肉羊产业技术体系专项（CARS-39）；国家星火计划项目（2015G750002）资助

Abstract

Abstract:

In order to determine the nutrient content of native herbage rapidly in Inner Mongolia Xilin Gol League rangeland, 407 samples of main species and the mixed herbage were collected from May to November in 2016. This study utilized the modified partial least squares (MPLS) to get the calibration of crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (ADL), crude ash (Ash), crude fat (EE), calcium (Ca), phosphorus (P) by combing near-infrared reflectance full spectrum scanning technology with the laboratory test data. The results show that the relative error of external verification (RPD (SD/SEP) of Ca, NDF, DM, CP and Ash is greater than 3, the correlation coefficient RSQ between the value predicted by NIRS and chemical method is greater than 0.9, which indicated that calibration of Ca, NDF, DM, CP, Ash is good, the establishment of the calibration model can be used for practical detection; The quantitative analysis of the components by employing NIRS is feasible as the relative error of external verification of ADF is 2.5

Key words: Near infrared spectroscopy, Natural herbage, Nutritional composition, Quantitative analysis

摘要：

为了快速测定内蒙古锡林郭勒盟草原天然牧草的营养成分，试验选用内蒙古锡林郭勒盟草原2016年5—11月份的主要牧草及混合牧草样品共407份，研究利用近红外漫反射全光谱扫描技术结合实验室检测数据，用修正偏最小二乘法（MPLS），进行粗蛋白（CP）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）、木质素（ADL）、粗灰分（Ash）、粗脂肪（EE）、钙（Ca）、磷（P）的定标和验证。结果表明：Ca、NDF、DM、CP、Ash的外部验证相对分析误差RPD（SD/SEP）均 > 3，NIRS预测值与化学值的相关系数RSQ均在0.9以上，说明这5个指标的定标效果较好，建立的定标模型可以用于实际检测；ADF外部验证相对分析误差2.5

关键词: 近红外光谱技术, 天然牧草, 营养成分, 定量分析

CLC Number:

S812

LIU Zhe, WANG Yu-qin, XUE Shu-yuan, JIN Hai, LI Yuan-xiao, WANG Jian-ping, LI Fa-di, ZHANG Pan-pan. Quantitative Analysis of Herbage Nutritional Components on Natural Pasture by Near Infrared Diffuse Reflectance Spectroscopy[J]. Acta Agrestia Sinica, 2018, 26(1): 249-255.

刘哲, 王玉琴, 薛树媛, 金海, 李元晓, 王建平, 李发弟, 张盼盼. 近红外漫反射光谱定量分析天然牧草营养成分[J]. 草地学报, 2018, 26(1): 249-255.

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Quantitative Analysis of Herbage Nutritional Components on Natural Pasture by Near Infrared Diffuse Reflectance Spectroscopy

近红外漫反射光谱定量分析天然牧草营养成分

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