Analysis of Differences and Influencing Factors of Plant δ13C in Leymus chinensis Grassland in Inner-Mongolia,China

doi:10.11733/j.issn.1007-0435.2019.01.020

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (1): 153-162.DOI: 10.11733/j.issn.1007-0435.2019.01.020

Previous Articles Next Articles

Analysis of Differences and Influencing Factors of Plant δ¹³C in Leymus chinensis Grassland in Inner-Mongolia,China

GENG Yuan-bo¹, WANG Zi-teng^1,2, LI Ru-xia^1,2

1. Institute of Geographic Sciences and Natural Resources Research, CAS, Beijing 100101, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2018-05-03 Revised:2019-01-02 Online:2019-02-15 Published:2019-04-13

δ¹³C值在羊草草原植物体中的差异和变化及其影响因素分析

耿元波¹, 王子腾^1,2, 李茹霞^1,2

1. 中国科学院地理科学与资源研究所, 北京 100101;
2. 中国科学院大学, 北京 100049

通讯作者: 耿元波,E-mail:gengyb@igsnrr.ac.cn
作者简介:耿元波(1969-),男,新疆霍城县人,博士,副研究员,主要从事环境生物地球化学方面的研究,E-mail:gengyb@igsnrr.ac.cn
基金资助:
国家自然科学基金项目（41071138）资助

Abstract

Abstract: In order to understand the distribution and change of stable carbon isotope¹³C in grassland plants and the effects of soil moisture and temperature on the stable carbon isotope¹³C composition (δ¹³C),we measured the δ¹³C of shoots and living roots which both had two treatments,¹³C pulse labeling treatment and control treatment,and analyzed the effect of soil moisture content,air temperature and ground temperature on the δ¹³C of plants body during the fast-growing stage (July-September) of the Leymus chinensis grassland in Inner Mongolia. The results showed that the δ¹³C of the living roots was 1~2‰ higher than the δ¹³C of the shoots in control treatments,and the δ¹³C of the shoots in¹³C pulse labeling treatment was significantly greater than the δ¹³C of the living roots. The variation trends of the δ¹³C value of shoots and living roots in labeling and control treatments were both in good consistency with time,which showed that the value of¹³C in shoots and living roots decreased at first,then increased,and then decreased during the growing season of plants. The δ¹³C of the shoots and living roots in control group and living roots in¹³C pulse labeling treatment had a linear negative correlation with soil moisture content in the layer of 0~20 cm,but the δ¹³C of shoots in¹³C pulse labeling treatment had Quadratic function relation with the soil moisture content in the layer of 0~20 cm,which indicated that the δ¹³C increased with increasing soil moisture content when it had a low soil moisture content,and the δ¹³C decreased when there was a high soil moisture content. In¹³C pulse labeling treatment and control treatment,there were significant positive correlation between temperature and the δ¹³C of the shoots and living roots,the δ¹³C of the shoots and living roots increased with increasing the temperature.

Key words: Leymus chinensis grassland, ¹³C pulse labeling, δ¹³C, Soil moisture content, Air temperature, Soil temperature

摘要： 为了解稳定同位素¹³C在草原植物体中的分配和变化以及水分和温度对¹³C组成（δ¹³C）的影响，在内蒙古羊草草原的生长盛期（7-9月），应用¹³C脉冲标记技术，测定了¹³C脉冲标记处理和对照处理下羊草草原植物活体和活根中的δ¹³C，分析了土壤水分、气温和地温对植物体中δ¹³C的影响。结果表明：对照处理中羊草草原植物活根δ¹³C值比活体高1~2‰，标记处理中活体δ¹³C值显著高于活根，标记处理中活体和活根的δ¹³C均显著高于对照处理。生长盛期，标记和对照处理中植物活体和活根的δ¹³C值均表现为先减小再增加，随后再减小的趋势。对照处理中活体和活根以及标记处理中活根的δ¹³C与0~20 cm土壤水分含量之间线性负相关，而标记处理中活体δ¹³C与0~20 cm土壤水分之间存在二次函数关系。标记、对照两处理中羊草草原活体、活根δ¹³C与温度均呈线性正相关。

关键词: 羊草草原, δ¹³C, ¹³C脉冲标记, 土壤水分, 气温, 土壤温度

CLC Number:

GENG Yuan-bo, WANG Zi-teng, LI Ru-xia. Analysis of Differences and Influencing Factors of Plant δ¹³C in Leymus chinensis Grassland in Inner-Mongolia,China[J]. Acta Agrestia Sinica, 2019, 27(1): 153-162.

耿元波, 王子腾, 李茹霞. δ¹³C值在羊草草原植物体中的差异和变化及其影响因素分析[J]. 草地学报, 2019, 27(1): 153-162.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2019.01.020

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2019/V27/I1/153

References

[1] 赵丹,程军回,刘耘华,等.荒漠植物梭梭稳定碳同位素组成与环境因子的关系[J].生态学报,2017,37(8):2743-2752
[2] Wang G A,Han J M,Liu D S.The carbon isotope composition of c₃ herbaceous plants in loess area of northern china[J].Science in China (Series D),2003,46(10):1069-1076
[3] Zhang C G,Chen F H,Jin M.Study on modern plant C-13 in western china and its significance[J].Chinese Journal of Geochemistry,2003,22(2):97-106
[4] 李军,张成君,李瑞博,等.中国西部地区水-热综合环境因素对植物碳同位素组成的影响[J].兰州大学学报,2013(6):752-758
[5] 苏波,韩兴国,李凌浩,等.中国东北样带草原区植物δ¹³c值及水分利用效率对环境梯度的响应[J].植物生态学报,2000,24(6):648-655
[6] 杨世超,邓波,张蕴薇,等.中苜一号紫花苜蓿碳同化分配与转移速率的研究[J].草地学报,2012,20(04):679-685
[7] Kohn M J.Carbon isotope compositions of terrestrial c3 plants as indicators of (paleo) ecology and (paleo) climate[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(46):19691-19695
[8] Liu X Z,Su Q,Li C K,et al.Responses of carbon isotope ratios of c₃ herbs to humidity index in northern china[J].Turkish Journal of Earth Sciences,2014,23:100-111
[9] Wang G A,Li J Z,Liu X Z,et al.Variations in carbon isotope ratios of plants across a temperature gradient along the 400mm isoline of mean annual precipitation in north china and their relevance to paleovegetation reconstruction[J].Quaternary Science Reviews,2013,63(1):83-90
[10] Terwilliger V J,Eshetu Z,Colman A,et al.Reconstructing palaeoenvironment from δ¹³c and δ¹⁵n values of soil organic matter:A calibration from arid and wetter elevation transects in ethiopia[J].2008,147(3):197-210
[11] Diefendorf A F,Mueller K E,Wing S L,et al.Global patterns in leaf¹³c discrimination and implications for studies of past and future climate[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(13):5738-5743
[12] 张蕊,赵钰,何红波,等.基于稳定碳同位素技术研究大气co2浓度升高对植物-土壤系统碳循环的影响[J].应用生态学报,2017,28(7):2379-2388
[13] 司高月,李晓玉,程淑兰,等.长白山垂直带森林叶片-凋落物-土壤连续体有机碳动态——基于稳定性碳同位素分析[J].生态学报,2017,37(16):5285-5293
[14] 何树斌,王燚,程宇阳,等.丛枝菌根真菌与柳枝稷协同固碳机制及对土壤碳氮循环的调控[J].草地学报,2016,24(04):802-806
[15] 王喜明,王媛,马丽娜,等.碳、氮同位素技术在我国草地植物营养研究中的应用[J].中国草地学报,2014,36(1):104-108
[16] Bruggemann N,Gessler A,Kayler Z,et al.Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum:A review[J].Biogeosciences,2011,8(11):3457-3489
[17] Creamer C A,Filley T R,Boutton T W,et al.Controls on soil carbon accumulation during woody plant encroachment:Evidence from physical fractionation,soil respiration,and δ¹³c of respired co₂[J].Soil Biology&Biochemistry,2011,43(8):1678-1687
[18] Zou J,Zhao L,Xu S,et al.Field¹³co₂ pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a kobresia meadow[J].Biogeosciences,2014,11:4381-4391
[19] Resco V,Ferrio J P,Carreira J A,et al.The stable isotope ecology of terrestrial plant succession[J].2011,4(2-3):117-130
[20] Tucker C L,Young J M,Williams D G,et al.Process-based isotope partitioning of winter soil respiration in a subalpine ecosystem reveals importance of rhizospheric respiration[J].Biogeochemistry,2014,121(2):389-408
[21] 宋文琛,同小娟,张劲松,等.用自然¹³C丰度法区分人工林根源呼吸的原理与应用[J].中国水土保持科学,2015,13(4):37-43
[22] Riederer M,Pausch J,Kuzyakov Y,et al.Partitioning nee for absolute c input into various ecosystem pools by combining results from eddy-covariance,atmospheric flux partitioning and 13co2 pulse labeling[J].Plant and Soil,2015,390(1-2):61-76
[23] Zhang J M,Griffis T J,Baker J M.Using continuous stable isotope measurements to partition net ecosystem co 2 exchange[J].Plant Cell&Environment,2006,29(4):483-496
[24] Still C J,Berry J A,Collatz G J,et al.Global distribution of C₃ and C₄ vegetation:Carbon cycle implications[J].Global Biogeochemical Cycles,2003,17(1):1006
[25] O'leary M H.Carbon isotopes in photosynthesis[J].Bioscience,1988,38(5):328-336
[26] Coleman D C,Fry B.Carbon isotope techniques[M].San Diego:Academic Press,1991
[27] Kuzyakov Y.Sources of co₂ efflux from soil and review of partitioning methods[J].Soil Biology&Biochemistry,2006,38:425-448
[28] Craig H.The geochemistry of the stable carbon isotopes[J].Geochimica et Cosmochimica Acta,1953,3(2):53-92
[29] Badeck F W,Tcherkez G,Nogués S,et al.Post-photosynthetic fractionation of stable carbon isotopes between plant organs——a widespread phenomenon[J].Rapid Communications in Mass Spectrometry,2005,19(11):1381-1391
[30] Wu Y B,Tan H C,Deng Y C,et al.Partitioning pattern of carbon flux in a kobresia grassland on the qinghai-tibetan plateau revealed by field 13c pulse-labeling[J].Global Change Biology,2010,16(8):2322-2333
[31] 刘贤赵,宿庆,李嘉竹,等.控温条件下c₃、c₄草本植物碳同位素组成对温度的响应[J].生态学报,2015,35(10):3278-3287
[32] 殷树鹏,张成君,郭方琴,等.植物碳同位素组成的环境影响因素及在水分利用效率中的应用_殷树鹏[J].同位素,2008,21(1):46-52
[33] Zhou Y C,Fan J W,Zhong H P,et al.Relationships between altitudinal gradient and plant carbon isotope composition of grassland communities on the qinghai-tibet plateau,china[J].SCIENCE CHINA (Earth Sciences),2013,56(2):311-320
[34] 赵艳艳,徐隆华,姚步青,等.模拟增温对高寒草甸植物叶片碳氮及其同位素δ¹³c和δ¹⁵n含量的影响[J].西北植物学报,2016,36(4):777-783
[35] 刘艳杰,许宁,牛海山.内蒙古草原常见植物叶片δ¹³c和δ¹⁵n对环境因子的响应[J].生态学报,2016,36(1):235-243
[36] 严昌荣,韩兴国.六种木本植物水分利用效率和其小生境关系研究[J].生态学报,2001,21(11):1952-1956
[37] Swap R J,Aranibar J N,Dowty P R,et al.Natural abundance of ¹³C and ¹⁵n in C₃ and C₄ vegetation of southern africa:Patterns and implications[J].Global Change Biology,2004,10(3):350-358
[38] Hartman G,Danin A.Isotopic values of plants in relation to water availability in the eastern mediterranean region[J].Oecologia,2010,162(4):837-52
[39] 齐玉春,董云社,耿元波,等.我国草地生态系统碳循环研究进展[J].地理科学进展,2003,22(4):342-352
[40] Li S G,Asanuma J,Eugster W,et al.Net ecosystem carbon dioxide exchange over grazed steppe in central mongolia[J].Global Change Biology,2005,11(11):1941-1955
[41] 程迁,莫兴国,王永芬,等.羊草草原碳循环过程的模拟与验证[J].自然资源学报,2010,25(1):60-70
[42] Chen D M,Li J J,Lan Z C,et al.Soil acidification exerts a greater control on soil respiration than soil nitrogen availability in grasslands subjected to long-term nitrogen enrichment[J].Functional Ecology,2016,30(4):658-669
[43] 李博,雍世鹏,李忠厚.锡林河流域植被及其利用[C]//中国科学院内蒙古草原生态系统定位站编.草原生态系统研究(第3集).北京:科学出版社,1988:84-183
[44] 汪久文,蔡蔚琪.锡林河流域土壤的发生类型及其性质的研究[C]//中国科学院内蒙古草原生态系统定位站编.草原生态系统研究(第3集).北京:科学出版社,1988:23-83
[45] 龚自同等.中国土壤系统分类:理论·方法·实践[M].北京:科学出版社,1999:442-487
[46] 耿元波,章申,董云社,等.草原土壤的碳氮含量及其与温室气体通量的相关性[J].地理学报,2001,56(1):44-53
[47] Wang Z P,Li L H,Han X G,et al.Dynamics and allocation of recently photo-assimilated carbon in an inner mongolia temperate steppe[J].Environmental&Experimental Botany,2007,59(1):1-10
[48] 鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,2000:14-24
[49] Skrzypek G,Kałużny A,WojtuńB,et al.The carbon stable isotopic composition of mosses:A record of temperature variation[J].2007,38(10):1770-1781
[50] Korol R L,Kirschbaum M U,Farquhar G D,et al.Effects of water status and soil fertility on the c-isotope signature in pinus radiata[J].Tree Physiology,1999,19(9):551-562
[51] Farquhar G D,Ehleringer J R,Hubick K T.Carbon isotope discrimination and photosynthesis[J].Annual Review of Plant Physiology,1989,40(1):503-537
[52] Farquhar G D,O'leary M H,Berry J A.On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves[J].Australian Journal of Plant Physiology,1982,9(2):121-137
[53] Yun S-I,Ro H-M.Stable c and n isotopes:A tool to interpret interacting environmental stresses on soil and plant[J].Journal of Applied Biological Chemistry,2008,51(6):262-271
[54] Kwak J-H,Lee K-S,Lim S-S,et al.Historical responses of quercus variabilis growth to environmental changes in southern korea:Evidence from tree ring width and delta c-13[J].Journal of the Korean Society for Applied Biological Chemistry,2013,56(5):583-590
[55] Cernusak L A,Tcherkez G,Keitel C,et al.Why are non-photosynthetic tissues generally 13c enriched compared with leaves in c3 plants? Review and synthesis of current hypotheses[J].Functional Plant Biology,2009,36(3):199-213
[56] 于秀丽,姜明.芦苇沼泽和羊草草甸土壤呼吸动态研究[J].中国农机化学报,2016,37(5):236-240
[57] Schleser G H,Helle G,Lücke A,et al.Isotope signals as climate proxies:The role of transfer functions in the study of terrestrial archives[J].Quaternary Science Reviews,1999,18(7):927-943

Analysis of Differences and Influencing Factors of Plant δ¹³C in Leymus chinensis Grassland in Inner-Mongolia,China

δ¹³C值在羊草草原植物体中的差异和变化及其影响因素分析

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Tong, ZHANG Yu, ZHAO Jin-ling, YAN Rui-rui, XIN Xiao-ping, WANG Xu, CHEN Jin-qiang, WU Dong-xiu, LI Ling-hao, ZHAO Man. Effects of Grazing Intensity on Soil Water-Heat Regime and Above-Ground Biomass in a Meadow Steppe [J]. Acta Agrestia Sinica, 2021, 29(11): 2577-2582.
[2]	ZHANG Yu-kun, WANG Hong-bin, ZHANG Da-cai. Analysis on the Differences of Soil Physicochemical Properties between Two Habitats in Alpine Meadow,Western Sichuan Plateau [J]. Acta Agrestia Sinica, 2020, 28(1): 207-213.
[3]	FU Hao-yu, JING Chang-qing, GUO Wen-zhang, CHEN-Chen, DENG Xiao-jin. Changes of Soil Respiration in Desert Shrub Grassland and Its Response to Soil Temperature and Humidity in Junggar Basin [J]. Acta Agrestia Sinica, 2019, 27(6): 1677-1684.
[4]	CHEN Ji-shan, KONG Xiao-lei, ZHU Rui-fen, ZHANG Qiang, DI Gui-li, KANG Xintong, LU Juan. Effects of Cutting Root and Nitrogen Application on Soil Physical and Chemical Properties of Degraded Leymus chinensis Grassland [J]. Acta Agrestia Sinica, 2018, 26(5): 1104-1108.
[5]	JING Yuan-yuan, HE Xiang, XU Chang-lin, YU Xiao-jun. Effects of Different Film-Mulching Planting Patterns on Soil Temperature and Water Moisture of Alfalfa Grassland in Eastern Qilian Mountain Area [J]. Acta Agrestia Sinica, 2018, 26(2): 321-329.
[6]	WANG Jian-li, HAO Jun, YANG Feng, LIU Hong-lai, CHENG Wei, CHEN Chao. Dynamic Variation of the Magnitude of Edge Influence of the Coupling of Soil Temperature and Moisture on Cultivated Grassland-cropland Boundary in Guizhou Province [J]. Acta Agrestia Sinica, 2017, 25(2): 303-309.
[7]	YANG Feng, LIU Hong-lai, CHEN Chao, LI Lin, KONG De-shun, WU Wen-xuan, ZHAO Li-li, XIANG Jun. Edge Influence of Soil Temperature on Cultivated Pasture-Farmland Boundary in Guizhou Province [J]. , 2013, 21(6): 1080-1084.
[8]	LIU Xiao-yong, WANG Wei, ZHAO Ming-xin, LI Hong-xu, LI Jian-ming, SUN Ding-hu. Effects of Mulching on the Growth Rhythm and Fruit Characters of Pear in Longdong Dryland Regions [J]. Acta Agrestia Sinica, 2013, 21(5): 958-964.
[9]	YUAN Fei, ZHAO Min, RONG Yu-ping, BAI Ke-yu. Effect of Raking on Grassland Soil Respiration [J]. , 2012, 20(4): 637-642.
[10]	YANG Shi-chao, DENG Bo, ZHANG Yun-wei, YANG Fu-yu. Absorption and Transfer of Photosynthetic Carbon in Alfalfa (Zhongmu No. 1) [J]. , 2012, 20(4): 679-685.
[11]	GAO Wei-ping, ZHOU He, XIONG Xue. Effect of Mowing Frequency on Characteristics of Soil Respiration of Mixed Turfgrass [J]. Acta Agrestia Sinica, 2012, 20(3): 471-478.
[12]	CHAI Qing, ZHONG Fang, LI Chun-jie, NAN Zhi-biao. Effects of Biofertilizer on Growth of Three Landscaping Grasses under Different Soil Moisture Contents [J]. , 2010, 18(6): 854-858.
[13]	XIE Hui-hui, FAN Jun, HAO Ming-de, WANG Jian-guo, QI Li-bin. Effects of Soil Surface Mulching on the Water Use of Artificial Grassland in the Water-Wind Erosion Crisscross Region of the Loess Plateau [J]. , 2010, 18(2): 172-176,182.
[14]	WANG Guo-liang, SHENG Yi-bing, HE Feng, WAN Li-qiang, LI Xiang-lin. Study on Seasonal Dynamics of Aboveground Biomass in Leymus chinensis(Trin.) Tzvel.Grassland [J]. , 2010, 18(1): 11-15.
[15]	WANG Guo-liang, LI Xiang-lin, WAN Li-qiang, HE Feng, WANG Hu. Effect of Cutting on the Growth Capability of Leymus chinensis Grassland [J]. , 2007, 15(3): 263-268.