Abstract: To explore and validate the efficient hybrid breeding technology of herbicide-tolerant transgenic alfalfa,one control group was conducted using the alfalfa ‘GN-1’ as the male and female parents;11 hybrid combinations were conducted using 11 glyphosate-resistant herbicide transgenic alfalfa as the male parent and the male sterile line MS-GN-1A as the female parent. The resistance of progeny plants was tested by test paper and PCR,and the yield and quality traits were evaluated and screened. We found that the progenies of 7 hybrid combinations showed excellent performance. Our research used male sterile lines as hybrid seeds to make the target traits of herbicide-tolerant transgenic alfalfa inherit efficiently and stability. Additionally,we have screened out excellent hybrid offspring,which provides a certain theoretical basis for seed breeding.

Key words: Transgenic alfalfa, Male sterility, Glufosinate resistant

摘要： 为探究抗除草剂转基因紫花苜蓿（Medicago sativa）的高效杂交种选育技术。本试验以‘公农1号’作为父母本配置了1个对照组合，以转Bar基因的抗草铵膦除草剂转基因紫花苜蓿作为父本，以雄性不育系MS-GN-1A作为母本，配置了11个杂交组合。对后代植株的抗性进行Bar试纸条、PCR检测，对产量和品质性状进行评价和筛选，结果表明，试验中有7个杂交组合的后代表现优异。此试验以紫花苜蓿雄性不育系作为母本配置杂交种，使抗除草剂转基因紫花苜蓿的目标性状高效且稳定遗传，同时在此试验基础上筛选出了优良杂交后代，为良种繁育提供了一定的理论基础。

关键词: 转基因紫花苜蓿, 雄性不育, 抗草铵膦