Effects of Exogenous Root Exudates on Symbiosis of Alfalfa and Rhizobium

doi:10.11733/j.issn.1007-0435.2020.01.010

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (1): 88-94.DOI: 10.11733/j.issn.1007-0435.2020.01.010

Previous Articles Next Articles

Effects of Exogenous Root Exudates on Symbiosis of Alfalfa and Rhizobium

YU Yue¹, LIU Jing¹, DENG Bo¹, LIU Zhong-kuan², LI Jia-huan¹, HE Yu-juan¹

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, Hebei Province 050051, China

Received:2019-09-16 Revised:2019-10-22 Online:2020-02-15 Published:2020-01-18

外源根系分泌物对紫花苜蓿根瘤菌共生体系的影响

于跃¹, 刘静¹, 邓波¹, 刘忠宽², 李佳欢¹, 何玉娟¹

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 河北省农林科学院农业资源环境研究所, 河北石家庄 050051

通讯作者: 邓波
作者简介:于跃(1993-)女,辽宁丹东人,硕士研究生,主要从事苜蓿根瘤菌研究,E-mail:1148166567@qq.com
基金资助:
国家农业产业技术体系项目（CARS-34）；国家重点研发计划"典型脆弱生态修复与保护研究（2016YFC0500603）"资助

Abstract

Abstract: In order to screen the extraneous root exudates for inducing growth and nodules of alfalfa,we analyzed the induction effect of five root exogenous exudates (symbiotic signaling substances) including Luteolin,Methyl Jasmonate,Tetronic acid and Stachydrine+Trigonelline mixture on alfalfa rhizobia ACCC17617 and ACCC17631 and analyzed the characteristics of nodule formation and the growth traits of alfalfa,both in laboratory and the field.The results showed that five exogenous root exudates effectively increased the nodule number,biomass and protein content of alfalfa,as well as the incidence of rhizobia. Different substances (or combinations) had different effects on the growth of alfalfa and bacterial strains. Luteolin promoted the growth and development of alfalfa. In the field experiment,the competitiveness of A31 increased more after adding root secretion than that of A17.

Key words: Alfalfa, Sinorhizobium meliloti, Root exudates

摘要： 为筛选诱导紫花苜蓿（Medicago sativa L.）生长结瘤的适宜外源根系分泌物，本试验利用木犀草素、茉莉酸甲酯、季酮酸以及水苏碱+葫芦巴碱混合物5种外源根系分泌物（共生信号物质）对苜蓿根瘤菌ACCC17617（A17）和ACCC17631（A31）诱导，结合室内试验与田间试验，分析外源根系分泌物对根瘤菌的诱导效应，并对苜蓿结瘤特征及生长性状测定，研究外源根系分泌物对紫花苜蓿接种根瘤菌共生体系的影响。结果表明：5种外源根系分泌物能有效提高苜蓿的结瘤数、生物量、蛋白含量以及受诱导的根瘤菌占瘤率。不同物质（或组合）对菌株与苜蓿共生结瘤和苜蓿生长的影响不同，室内条件下木犀草素对苜蓿的生长发育有较好的促进效果。田间试验条件下，经外源根系分泌物诱导后，根瘤菌A31的田间竞争力大于根瘤菌A17。

关键词: 紫花苜蓿, 根瘤菌, 根系分泌物

CLC Number:

S963.22+3.3

YU Yue, LIU Jing, DENG Bo, LIU Zhong-kuan, LI Jia-huan, HE Yu-juan. Effects of Exogenous Root Exudates on Symbiosis of Alfalfa and Rhizobium[J]. Acta Agrestia Sinica, 2020, 28(1): 88-94.

于跃, 刘静, 邓波, 刘忠宽, 李佳欢, 何玉娟. 外源根系分泌物对紫花苜蓿根瘤菌共生体系的影响[J]. 草地学报, 2020, 28(1): 88-94.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2020.01.010

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2020/V28/I1/88

References

[1] 谢勇,孙洪仁,张新全,等. 坝上地区紫花苜蓿氮、磷、钾肥料效应与推荐施肥量[J]. 中国草地学报,2012,34(2):52-57
[2] 孟凯,李星月,冀晓婷,等. 氮、磷、钾配施对草原3号苜蓿干草产量的影响[J]. 中国草地学报,2019,41(3):107-114
[3] 张英俊,任继周,王明利,等. 论牧草产业在我国农业产业结构中的地位和发展布局[J]. 中国农业科技导报,2013,15(4):61-71
[4] 董宁,赵伟. 牧草产品进口价格及进口量预测-以苜蓿草为例[J]. 草原与草业,2018,30(01):8-13
[5] 潘霞,高永,刘博,等. 苜蓿产业发展现状及前景展望[J]. 绿色科技,2017(13):104-107
[6] 李星月,米福贵,孟凯,肖燕子,韩亚琼. 施肥对土默特地区中苜2号干草产量的影响[J]. 中国草地学报,2017,39(02):26-32
[7] G. Bélanger,Richards J E. Dynamics of biomass and N accumulation of alfalfa under three N fertilization rates[J]. Plant and Soil,2000,219(1-2):177-185
[8] Zhang W F,Dou Z X,He P,et al. New technologies reduce greenhouse gas emissions from nitrogenous fertilizer in China[J]. Proceedings of the National Academy of Sciences,2013,110(21):8375-8380
[9] 王树起,韩晓增,乔云发,等. 施氮对大豆根瘤生长和结瘤固氮的影响[J]. 华北农学报,2009,24(2):176-179
[10] 王继朋. 紫花苜蓿接种根瘤菌的技术[J]. 农村科技,2004(10):24-24
[11] Brencic A,Winans S,Colonization P. (2005) Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria[J]. Microbiology & Molecular Biology Reviews Mmbr,2005,69(1):155-94
[12] Khan,Mohammad Saghir,Zaidi,Almas,Musarrat,Javed. Microbial Strategies for Crop Improvement||Functional Diversity Among Plant Growth-Promoting Rhizobacteria:Current Status[J]. 10.1007/978-3-642-01979-1(Chapter 6):105-132
[13] Peters N,Frost J,Long S. A plant flavone,luteolin,induces expression of Rhizobium meliloti nodulation genes[J]. Science,1986,233(4767):977-980
[14] Rosas S,Soria R,Néstor Correa,et al. Jasmonic acid stimulates the expression of nod genes in Rhizobium[J]. Plant Molecular Biology,1998,38(6):1161-1168
[15] Dakora F D,Phillips D A. Root exudates as mediators of mineral acquisition in low-nutrient environments[J]. Plant and Soil,2002,245(1):201-213
[16] Phillips D A,Maxwell J C A.Trigonelline and Stachydrine Released from Alfalfa Seeds Activate NodD2 Protein in Rhizobium meliloti[J]. Plant Physiology,1992,99(4):1526-1531
[17] Mabood F,Smith D L. Pre-incubation of Bradyrhizobium japonicum with jasmonates accelerates nodulation and nitrogen fixation in soybean (Glycine max) at optimal and suboptimal root zone temperatures[J]. Physiologia Plantarum,2005,125(3):311-323
[18] Begum A A,Leibovitch S,Migner P,et al. Inoculation of pea (Pisum sativum L.) by Rhizobium leguminosarum bv. viceae preincubated with naringenin and hesperetin or application of naringenin and hesperetin directly into soil increased pea nodulation under short season conditions[J]. Plant and Soil,2001,237(1):71-80
[19] Ghasem F,Poustini K,Besharati H,et al. Pre-incubation of sinorhizobium meliloti with luteolin,methyl jasmonate and genistein affecting alfalfa (Medicago sativa L.) growth,nodulation and nitrogen fixation under salt stress conditions.[J]. Pediatrics,2012,2003(2):294-330
[20] Cooper J E. Multiple Responses of Rhizobia to Flavonoids During Legume Root Infection[J]. Advances in Botanical Research,2004,41(41):1-62
[21] Liu C W,Murray J D. The Role of Flavonoids in Nodulation Host-Range Specificity:An Update:[J]. Plants,2016,5(3):33
[22] Zhang J,Subramanian S,Stacey G,et al. Flavones and flavonols play distinct critical roles during nodulation of Medicago truncatula by Sinorhizobium meliloti.[J]. Plant Journal,2010,57(1):171-183
[23] Mabood F,Zhou X M,Smith D. Bradyrhizobium japonieum preincubated with methyl jasmonate increase soybean nodulation and nitrogen fixation. Agronomy journal,2006,98(2):289-294
[24] Cooper J E. Early interactions between legumes and rhizobia:disclosing complexity in a molecular dialogue[J]. Journal of Applied Microbiology,2010,103(5):1355-1365
[25] Gagnon H,Ibrahim R K. Aldonic acids:A novel family of nod gene inducers of Mesorhizobium loti,Rhizobium lupine,and Sinorhizobium meliloti Molecular plant-microbe interactions,1998,11(10):988-998
[26] Phillips D A,Joseph C M,Maxwell C A. Trigonelline and Stachydrine Released from Alfalfa Seeds Activate NodD2 Protein in Rhizobium meliloti[J]. Plant Physiology,1992,99(4):1526-1531
[27] Macchiavelli R E,Brelles-Mari? o G. Nod factor-treated Medicago truncatula roots and seeds show an increased number of nodules when inoculated with a limiting population of Sinorhizobium meliloti[J]. Journal of Experimental Botany,2004,55(408):2635-2640
[28] 康俊梅,张丽娟,郭文山,等. 中苜1号紫花苜蓿高效共生根瘤菌的筛选[J]. 草地学报,2008,16(5):497-500
[29] 肖文丽. 大豆根瘤菌竞争结瘤机理及其主要影响因子的初步研究[D]. 北京:中国农业科学院,2010:36-42
[30] Ghasem F,Poustini K,Besharati H,et al. Pre-incubation of sinorhizobium meliloti with luteolin,methyl jasmonate and genistein affecting alfalfa (Medicago sativa L.) growth,nodulation and nitrogen fixation under salt stress conditions[J]. Pediatrics,2012,2003(2):294-330

Effects of Exogenous Root Exudates on Symbiosis of Alfalfa and Rhizobium

外源根系分泌物对紫花苜蓿根瘤菌共生体系的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Jing, LIU Xiao-jing, Cheng Tian-tian, Tong Chang-chun, WANG Xue. Study on Leaf Characteristics and Yield Effect of Alfalfa with Different Nitrogen Efficiency [J]. Acta Agrestia Sinica, 2021, 29(9): 1941-1949.
[2]	MENG Xuan-chen, MA Peng-cheng, MA Jie, DUAN Zhen, HAN Yang-yang, ZHANG Ji-yu. Study on the Effect of Mulching Furrow and Fertilization of Alfalfa in Semi-arid Areas of Loess Plateau [J]. Acta Agrestia Sinica, 2021, 29(9): 2098-2106.
[3]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[4]	LI Chun-pin, QIU Yi-hui, XIA Hou-yin, TAN Lu-na, XU Huan-yu, ZHANG Han-wen, GAO Jing-hui. Membership Function Analysis of Low Transpiration and Yield Traits in Alfalfa Multi-paternal Hybrid F₁Generation [J]. Acta Agrestia Sinica, 2021, 29(7): 1416-1422.
[5]	LI Tian-qi, LIN Zhi-ling, LU Xuan, HUANG Jia-yuan, YANG Ning, WO Ye, GAO Kai, LIU Xiao-ni, WANG Xian-guo. Effects of Irrigation Amount on Soil Available Nutrient Distribution and Leaching Loss of Alfalfa in Horqin Sandy Land [J]. Acta Agrestia Sinica, 2021, 29(7): 1454-1461.
[6]	LI Hong, LI Bo, FANG Zhi-jian, YANG Zhao, ZHANG Chao, FENG Cheng-long, JIN Shan-shan, WANG Si-qi, SHI Wan-ying. Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress [J]. Acta Agrestia Sinica, 2021, 29(7): 1469-1476.
[7]	LI Peng-zhen, ZHAO De-qin, DENG Bo, Yang Jun, Zhao Guo-hua, Zhao Liang. Effects of Biochar Addition and Drought Stress on Photosynthetic Efficiency and Growth of Alfalfa Inoculated with Rhizobia [J]. Acta Agrestia Sinica, 2021, 29(6): 1257-1264.
[8]	WANG Wen-jing, MA Dong-mei, ZHAO Li-juan, MA Qiao-li. Effects of 2,4-table Brassinolide on Enzyme Activity and Root ion Distribution and Absorption in Alfalfa Seedlings [J]. Acta Agrestia Sinica, 2021, 29(6): 1363-1368.
[9]	SUN Lei-lei, ZHAO Gui-qin, CHAI Ji-kuan, LIU Fu-yuan, Nie Xiumei, WANG Jun. Effects of Storage Methods and Time on Mould Quantity and Species in Alfalfa Hay [J]. Acta Agrestia Sinica, 2021, 29(5): 884-893.
[10]	HUANG Rong, YAO Bo, ZHANG Yu-juan, JIA Qian-ying, LI Xiang-yang, ZHANG Hong, ZHANG Zhen-fen. Effect of Pantoea agglomerans CQ10 Lipopolysaccharide on Seed Germination and Seedling Growth of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(5): 965-971.
[11]	CHEN Wei-dong, ZHANG Yu-xia, CONG Bai-ming, A Ma-er, TIAN Yong-lei, ZHANG Qing-xin, DU Xiao-yan. Effects of Potassium Fertilizer on MDA,SP Content and Antioxidant System of Alfalfa Root Neck [J]. Acta Agrestia Sinica, 2021, 29(4): 717-723.
[12]	XU Hong-yu, ZHEN Li-li, LI Yu-ying, DONG Kuan-hu, LI Xiang-lin. Effect of Freeze-drying Environment on Freezing Tolerance of Alfalfa Crowns [J]. Acta Agrestia Sinica, 2021, 29(4): 724-733.
[13]	LI Man-you, MA Zhong-ren, WANG Bing, YANG Yu-qi, SHEN Xiao-tian, CAO Li-juan, LI Xiao-yun, LAN Jian. Study on the Mixed Planting Mode of Two Alfalfa Varieties in Different Row Ratios in the Yellow River Irrigation Area in Ningxia [J]. Acta Agrestia Sinica, 2021, 29(4): 798-804.
[14]	HOU Hong-yan, SHEN Chen, HUO Wen-jie, LIU Qiang, ZHANG Shuan-lin, WANG Cong, CHEN Lei, XU Qing-fang, WANG Yong-xin, WANG Zhong-hao, GUO Gang. The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage [J]. Acta Agrestia Sinica, 2021, 29(4): 835-841.
[15]	CHEN De-kui, GUO Xiang, ZHENG Ming-yang, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. The Effects of Amomum Villosum Lour. Essential Oil on the Fermentation Quality of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(4): 855-860.