Physiological Adaptation Mechanism of Paspalum notatum in Seedling Stage to Different Phosphorus Concentrations

doi:10.11733/j.issn.1007-0435.2021.04.007

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (4): 684-693.DOI: 10.11733/j.issn.1007-0435.2021.04.007

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Physiological Adaptation Mechanism of Paspalum notatum in Seedling Stage to Different Phosphorus Concentrations

LIU Fang¹,HUANG Li-juan¹,WANG Pu-chang²,ZHAO Li-li^1,3*

1. Department of Pratacultural Science, Institute of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, China;
2. Institute of Grass Industry, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou Province 550006, China;
3. State Engineering Technology Institute for Karst Rocky Desertification Control, Guiyang, Guizhou Province 550001, China

Received:2020-09-29 Revised:2020-12-06 Published:2021-06-02
Contact: * E-mail:zhaolili_0508@163.com

巴哈雀稗苗期对不同浓度磷胁迫的生理适应机制

刘芳¹, 黄莉娟¹, 王普昶², 赵丽丽^1,3*

1.贵州大学动物科学学院草业科学系, 贵州贵阳 550025;
2.贵州省草业研究所, 贵州贵阳 550006;
3.国家喀斯特石漠化防治工程技术研究中心,贵州贵阳 550001

通讯作者: * E-mail:zhaolili_0508@163.com
作者简介:刘芳(1996-),女,贵州遵义人,硕士研究生,Email:3563308785@qq.com
基金资助:
国家重点研发计划(2016YFC0502607-04);国家自然科学基金(31702173);贵州省普通高等学校科技拔尖人才支持计划(黔教合KY字[2017]059)资助

Abstract

Abstract: In order to explore the optimal phosphorus concentration of Paspalum notatum and reveal its growth and physiological response mechanism to non-suitable phosphorus concentrations,this study used Paspalum notatum as the experimental material,we set up 5 phosphorus treatment levels (P1,2 μmol·L^-1;P2,20 μmol·L^-1;P3,200 μmol·L^-1;P4,600 μmol·L^-1;P5,1000 μmol·L^-1). The physiological indexes were measured after 10,20,and 30 days of phosphorus stress and the morphological indexes were measured after 30 days of stress. The results showed that:The total root length,number of root branches,number of root tips,root surface area,and total root volume were the largest at P2 phosphorus level;The average root diameter increases with increasing phosphorus levels;Proline (Pro) and root acid phosphatase (ACP) activities decreased with the increase of phosphorus level;The content of leaf area,leaf length,leaf width,leaf circumference,chlorophyll (Chl),soluble protein (SP) and soluble sugar (SS) was the highest in P4 phosphorus;Both the fresh weight root to shoot ratio and the dry weight root to shoot ratio were the highest at low phosphorus level (P2);Leaf electrical conductivity,malondialdehyde (MDA),catalase (CAT),peroxidase (POD) and superoxide dismutase (SOD) activities were the smallest in the P4 phosphorus level. Using the method of combining principal component analysis and fuzzy membership function to comprehensively evaluate,the P4 phosphorus level was the most suitable for the growth of Paspalum notatum.

Key words: Different phosphorus levels, Root system, Physiology, Leaves, Paspalum notatum

摘要： 为了探究巴哈雀稗(Paspalum notatum)的最适磷浓度,揭示其对非适宜磷浓度的生长和生理应答机制,本研究以巴哈雀稗为试验材料,设置5种磷水平(P1:2 μmol·L^-1;P2:20 μmol·L^-1;P3:200 μmol·L^-1;P4:600 μmol·L^-1;P5:1 000 μmol·L^-1),测定胁迫10,20,30 d后各生理指标和胁迫30 d后各形态指标。结果表明:总根长、根分枝数、根尖数、根表面积、总根体积在P2磷水平最大;根平均直径随着磷水平的升高而升高;脯氨酸和根系酸性磷酸酶活性随着磷水平的升高而降低;叶面积、叶长、叶宽、叶周长、叶绿素、可溶性蛋白和可溶性糖含量在P4磷水平最大;鲜重根冠比与干重根冠比均在低磷水平(P2)最大;叶片电导率、丙二醛含量、过氧化氢酶、过氧化物酶和超氧化物歧化酶活性在P4磷水平均最小。利用主成分分析和模糊隶属函数结合的方法综合评价表明,P4磷水平最适宜巴哈雀稗的生长。

关键词: 不同磷水平, 根系, 生理, 叶片, 巴哈雀稗

CLC Number:

Q945.1

LIU Fang,HUANG Li-juan,WANG Pu-chang,ZHAO Li-li. Physiological Adaptation Mechanism of Paspalum notatum in Seedling Stage to Different Phosphorus Concentrations[J]. Acta Agrestia Sinica, 2021, 29(4): 684-693.

刘芳, 黄莉娟, 王普昶, 赵丽丽. 巴哈雀稗苗期对不同浓度磷胁迫的生理适应机制[J]. 草地学报, 2021, 29(4): 684-693.

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Physiological Adaptation Mechanism of Paspalum notatum in Seedling Stage to Different Phosphorus Concentrations

巴哈雀稗苗期对不同浓度磷胁迫的生理适应机制

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